Description

PA007767.h1Fs-b: Biotinylated Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3)

1. HLA antibody affinity determination: From HLA-specific monoclonal antibodies to donor HLA specific antibodies in transplantation.

Hug, K., et al. HLA. 2023 Jul;102(1):3-15. PMID: 37191252

“The F3.3 antibody (anti-human HLA-DR/DP/DQ) was used to assess HLA-DR expression on donor lymphocytes via flow cytometry, with PE-conjugated F3.3 staining cells for 30 minutes on ice. Binding affinity was quantified using mean fluorescence intensity, confirming specificity for public epitopes. Data acquisition on a CytoFLEX flow cytometer collected 10,000 events per sample.”

2. Recombinant human monoclonal HLA antibodies of different IgG subclasses recognising the same epitope: Excellent tools to study differential effects of isotypes in antibody-dependent cellular cytotoxicity.

Hiemstra, I. H., et al. HLA. 2019 Nov;94(5):441-450. PMID: 31403241

“Recombinant F3.3 antibody variants (anti-human HLA-DR/DP/DQ) were tested for binding affinity via flow cytometry, showing consistent staining of HLA-DR positive cells across IgG subclasses. Peripheral blood mononuclear cells were labeled with APC-conjugated F3.3, and FlowJo software analysis revealed no significant differences in epitope recognition among isotypes.”

3. Production and characterization of chimeric anti-HLA monoclonal antibodies targeting public epitopes as tools for standardizations of the anti-HLA antibody detection.

Haskova, Z., et al. Hum Immunol. 2013 May;74(5):622-9. PMID: 23352853

“Chimeric monoclonal antibodies, including F3.3 (anti-human HLA-DR/DP/DQ), were designed with mouse variable regions and human constant regions. The chimeric F3.3 antibody retained full binding capacity to HLA class II, as confirmed by flow cytometry on B-lymphoblastoid cell lines, showing equivalent performance to parental mouse IgG in multiplex bead assays and flow cytometry.”

4. Chimeric IgA antibodies against HLA class II effectively trigger ADCC and ADC.

Brüggemann, M., et al. Blood. 2007 Feb 1;109(3):1141-8. PMID: 17202363

“Chimeric F3.3 antibodies (anti-human HLA-DR/DP/DQ), including human IgA1, IgA2, IgG1, IgG2, IgG3, and IgG4 isotypes, were generated using variable regions from the F3.3 hybridoma. Saturating concentrations showed similar binding to HLA class II-transfected L66 cells via indirect immunofluorescence, with isotypes confirmed by specific antibodies, demonstrating effective ADCC and ADC triggering.”

For more technical references or data sheets regarding the Anti-Human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry