Description

PA007760.h1Fs-b: Biotinylated Recombinant Anti-human CD47 Monoclonal Antibody (Clone: B6H12), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32)

1. Mass spectrometric profiling of HLA-B44 peptidomes provides evidence for tapasin-mediated tryptophan editing

Kaur, A., et al. J Immunol. 2023. PMID: 37737643

“This study uses mass spectrometry to explore tapasin-mediated tryptophan editing in HLA-B44 peptidomes. The W6/32 antibody (anti-human HLA-ABC) was purified from mouse ascites and crosslinked to PureProteome Protein A magnetic beads for immunoaffinity purification. Cells were stained with W6/32-FITC antibody for surface HLA class I analysis via quantitative flow cytometry, revealing a significant reduction in HLA-B44:05 molecules on tapasin knockout cells compared to wild-type cells. A slight, non-significant decrease in HLA-B44:05 surface stability was observed in tapasin knockout cells in the presence of brefeldin A.”

2. Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins

Zhang, F., et al. Proc Natl Acad Sci U S A. 2022. PMID: 36136978

“This study investigates how sarbecovirus ORF7a proteins inhibit MHC-I antigen presentation. Human 293T, HuH7.5, U2OS, and A549/ACE2 cells were transduced with lentiviral vectors expressing SARS-CoV-2 ORF7a or Nef and stained with the pan-HLA-ABC antibody W6/32 for flow cytometry. MHC-I surface levels, measured by median fluorescence intensity, were reduced in ORF7a-expressing cells. The study compared wild-type and ORF7-deleted SARS-CoV-2 strains, confirming ORF7a’s role in suppressing MHC-I expression.”

3. Assessments of HLA-I Specificities of Anti-HLA-I Monoclonal Antibodies Using Solid Phase Bead Arrays

Zaitouna, A. J., et al. Bio Protoc. 2020. PMID: 33659325

“This study evaluates anti-HLA-I monoclonal antibody specificities using solid phase bead arrays. The W6/32 antibody, a pan anti-HLA class I antibody, was used to normalize HLA-I protein levels on Labscreen and FlowPRA Single Antigen Beads. Biotinylated W6/32 was tested at various dilutions (undiluted to 1:100) alongside anti-Bw4 and anti-Bw6 antibodies. Representative data showed W6/32 binding to HLA-I beads, with isotype control IgG2a used for comparison.”

4. Strategies for the measurements of expression levels and half-lives of HLA class I allotypes

Raghavan, M., et al. Hum Immunol. 2019. PMID: 30735755

“This study describes methods for measuring HLA class I expression and half-lives using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry histograms showed W6/32 signals for monocytes, NK cells, B cells, CD8+ T cells, and CD4+ T cells, with geometric mean values quantifying HLA class I expression. The W6/32 antibody was chosen for its broad recognition of most HLA class I allotypes, facilitating consistent assessment across healthy donors.”

5. Higher human lymphocyte antigen class I expression in early-stage cancer cells leads to high sensitivity for cytotoxic T lymphocytes

Akazawa, Y., et al. Cancer Sci. 2019. PMID: 30973665

“This study examines HLA class I expression in early-stage hepatocellular carcinoma (HCC) using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry analysis of tissue from five HCC patients compared HLA class I expression between cancer cells and non-tumorous hepatocytes. The mean fluorescence intensity ratio of W6/32 staining indicated higher HLA class I expression in cancer cells, enhancing sensitivity to cytotoxic T lymphocytes. Heterogeneous expression was observed in non-tumorous hepatocytes in one case.”

For more technical references or data sheets regarding the Recombinant Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.
Syd Labs provides the following in vivo grade recombinant anti-human CD47 monoclonal antibodies:
Recombinant Anti-human CD47 monoclonal antibody (Clone: B6H12)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD47 monoclonal antibodies:
Recombinant Anti-mouse CD47 monoclonal antibody (Clone: Miap301)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD370 / CD47 bispecific antibodies:
Recombinant Anti-mouse CD370 / CD47 bispecific antibody (Clone: 10B4 / A4)