Description

PA007768.h1Fs-b: Biotinylated Recombinant Anti-human HLA-DR Monoclonal Antibody (Clone: L243), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Human HLA-DR Monoclonal Antibody (Clone: L243)

1. Identifying major histocompatibility complex class II-DR molecules in bovine and swine peripheral blood monocyte-derived macrophages using mAb-L243

Celis-Giraldo, C., et al. Vaccine. 2024 May 31. PMID: 38631956

“The L243 monoclonal antibody (anti-human HLA-DR) was used to identify MHC-II-DR molecules in bovine and swine macrophages via flow cytometry, mass spectrometry, and immunopurification. The study demonstrated cross-reactivity of L243 with bovine and swine DR class II molecules, validating its use for immunopeptidomics to identify Staphylococcus aureus and Salmonella enterica peptides presented by MHC-II-DR.”

2. HLA class II antibodies induce necrotic cell death in human endothelial cells via a lysosomal membrane permeabilization-mediated pathway

Aljabri, A., et al. Cell Death Dis. 2019 Mar 8. PMID: 30850581

“The L243 antibody (anti-human HLA-DR) induced cell death in IFN-γ-stimulated human endothelial cells after 3 hours, as shown by annexin V/propidium iodide and LIVE/DEAD staining. Preincubation with soluble recombinant HLA-DR confirmed L243’s specific binding to HLA-DR, demonstrating its cytotoxic effect in the absence of complement, unlike its proliferative effect after 48 hours.”

3. Combating non-Hodgkin lymphoma by targeting both CD20 and HLA-DR through CD20–243 CrossMab

Zhao, L., et al. MAbs. 2014 May-Jun;6(3):740-8. PMID: 24670986

“The L243 antibody (anti-human HLA-DR) served as a positive control for HLA-DR binding in flow cytometry, showing comparable binding affinity to CD20-243 CrossMab on Raji cells. L243 competition confirmed specific HLA-DR epitope binding, with flow cytometry revealing enhanced internalization upon co-engagement of CD20 and HLA-DR.”

4. A naturally processed HLA-DR-bound peptide from the IL-9 receptor alpha of HTLV-1-transformed T cells serves as a T helper epitope

Kobayashi, H., et al. Cancer Immunol Immunother. 2012 Dec. PMID: 22638550

“The L243 antibody (anti-human HLA-DR) was used to immunoprecipitate HLA-DR molecules from HTLV-1-transformed T cells, with eluted peptides analyzed by mass spectrometry. Flow cytometry with L243 confirmed high HLA-DR surface expression, and L243 blocked T cell responses, verifying HLA-DR-restricted presentation of an IL-9 receptor alpha epitope.”

5. Characterization of a humanized IgG4 anti-HLA-DR monoclonal antibody that lacks effector cell functions but retains direct antilymphoma activity and increases the potency of rituximab

Stein, R., et al. Blood. 2006 Oct 15. PMID: 16778139

“The L243 antibody (anti-human HLA-DR), among other murine anti-HLA class II mAbs, demonstrated antibody-dependent cellular cytotoxicity (ADCC) in lymphoma cells. A humanized IgG4 version of L243 retained direct antilymphoma activity without effector functions, enhancing rituximab’s potency in targeting HLA-DR-expressing cells.”

For more technical references or data sheets regarding the Recombinant Anti-Human HLA-DR Monoclonal Antibody (Clone: L243) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry