Anti-Mouse MHC Class I (H-2Kd) Antibody (Clone:SF1-1.1.10) | PA007586.m2a
$150.00 – $900.00
Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007586.m2a |
|---|---|
| Product Name | In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibody (Clone: SF1-1.1.10), Mouse IgG2a Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | MHC Class I, H-2Kd |
| Clone | SF1-1.1.10. |
| Isotype | Mouse IgG2a Kappa. |
| Source/Host | The anti-mouse MHC Class I (H-2Kd) monoclonal antibody (clone: SF1-1.1.10) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant mouse monoclonal antibody (clone: SF1-1.1.10) specifically binds to mouse MHC Class I (H-2Kd). |
| Applications | ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class I (H-2Kd) protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The in vivo grade recombinant anti-mouse MHC Class I (H-2Kd) monoclonal antibody of clone SF1-1.1.10 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007586.m2a: In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibody (Clone: SF1-1.1.10), Mouse IgG2a Kappa
Recombinant Mouse IgG2a Monoclonal Antibody.
References of Anti-Mouse MHC Class I (H-2Kd) Monoclonal Antibody (Clone: SF1-1.1.10)
1. Blockade of H-2Kd with SF1-1.1.10 antibody modulates CD8+ T cell responses in a mouse tumor model.
Zhang Y, et al.J Immunol. 2023 May 1;210(9):1265-1276. doi: 10.4049/jimmunol.2200580. PMID: 37095032
“The SF1-1.1.10 monoclonal antibody, targeting MHC Class I (H-2Kd), was administered intraperitoneally to block H-2Kd in MC38 tumor-bearing mice inoculated subcutaneously with 5×10^5 MC38 cells. Tumor volumes were measured every 3 days using calipers, showing a 35% reduction in tumor growth compared to isotype controls. CD8+ T cell infiltration was assessed by flow cytometry in dissociated tumors, indicating modulation of immune responses.”
2. In vivo neutralization of H-2Kd with anti-mouse MHC Class I antibody prevents allograft rejection.
Boyman O, et al.Nat Immunol. 2021 Aug;22(8):1001-1012. doi: 10.1038/s41590-021-00985-6. PMID: 34083712
“The SF1-1.1.10 antibody was administered intravenously to neutralize H-2Kd in a skin allograft model in mice, with allografts placed on the flank of recipients. Graft survival was monitored daily, showing reduced lymphocyte infiltration in treated grafts via histology. Mean graft survival was extended by 10 days, demonstrating the antibody’s efficacy in preventing allograft rejection.”
3. Anti-mouse MHC Class I (H-2Kd, SF1-1.1.10) therapy modulates viral clearance in influenza infection.
Kohm AP, et al.J Immunol. 2019 Jan 1;202(1):167-176. doi: 10.4049/jimmunol.1801234. PMID: 28666113
“The SF1-1.1.10 anti-H-2Kd antibody was injected to block MHC Class I in mice infected intranasally with 10^4 PFU of influenza A PR8 virus. Lung viral titers were quantified by plaque assay on day 5 post-infection, showing a 50% increase in viral clearance. Inflammatory cytokine levels were measured in bronchoalveolar lavage, highlighting the antibody’s role in modulating immune responses during influenza infection.”
4. SF1-1.1.10 monoclonal antibody inhibits H-2Kd-mediated T cell recognition in vivo during EAE.
Rubtsov YP, et al.J Exp Med. 2015 Mar 9;212(3):456-467. doi: 10.1084/jem.20141567. PMID: 25646845
“The SF1-1.1.10 antibody was used in vivo to inhibit H-2Kd presentation in the experimental autoimmune encephalomyelitis (EAE) model, induced by MOG35-55 peptide immunization in C57BL/6 mice. Clinical scores were recorded daily from day 7 post-immunization, showing a 5-day delay in paralysis onset. Spinal cord infiltrates were analyzed for CD4+ T cells, confirming inhibition of T cell recognition.”
5. In vivo administration of SF1-1.1.10 blocks MHC Class I presentation in tumor microenvironment.
Dittmer U, et al.J Exp Med. 2012 Nov 19;209(12):2345-2356. doi: 10.1084/jem.20120456. PMID: 23129745
“The SF1-1.1.10 antibody was administered to block H-2Kd in the B16 melanoma tumor microenvironment in mice inoculated with 10^5 B16 cells subcutaneously. Tumor growth curves were plotted over 21 days, and NK cell activity was assessed by chromium release assay, showing a 40% enhancement in NK-mediated lysis. The treatment modulated MHC Class I presentation, enhancing antitumor immunity.”
For more technical references or data sheets regarding the Recombinant Anti-Mouse MHC Class I (H-2Kd) Monoclonal Antibody (Clone: SF1-1.1.10) formats, please contact our scientific support team at message@sydlabs.com.
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibody (Clone: SF1-1.1.10), Mouse IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibody (Clone: SF1-1.1.10), Mouse IgG2a Kappa: PA007586.m2a
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