Anti-Human HLA class II DR/DQ Antibody (Clone: 9.3F10) | PA007478
$200.00 – $1,250.00
Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007478 |
|---|---|
| Product Name | In vivo Grade Recombinant Anti-Human HLA class II DR/DQ Monoclonal Antibody, Mouse IgG2a Kappa (Clone: 9.3F10) |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | beta1 domain MHC class II HLA DPB, CELIAC1, class II HLA beta chain, MHC class II HLA-DR, DP, DQ |
| Clone | 9.3F10. |
| Isotype | Mouse IgG2a Kappa. |
| Specificity/Sensitivity | The in vivo grade recombinant mouse monoclonal antibody (clone: 9.3F10) specifically binds to human HLA class II DR/DQ. |
| Applications | ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human HLA class II DR/DQ protein. |
| Form Of Antibody | 0.2 μM filtered solution of 1x PBS. |
| Endotoxin | Less than 1 EU/mg of protein as determined by LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The in vivo grade recombinant anti-human HLA class II DR/DQ antibodies (clone of 9.3F10) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007478: Recombinant Anti-Human HLA class II DR/DQ Monoclonal Antibody (Clone: 9.3F10), Mouse IgG2a Kappa, In vivo Grade
The in vivo grade recombinant anti-human HLA class II DR/DQ Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells.
References of Anti-Human HLA-DR/DQ Monoclonal Antibody (Clone: 9.3F10)
1. Crossreactivity of a human autoimmune TCR is dominated by a single human leukocyte antigen residue.
Newell, E., et al.J Clin Invest. 2014 Oct;124(10):4277-89. doi: 10.1172/JCI73688. PMID: 25225671
“The study utilized anti-HLA-DR/DQ antibody (9.3F10) and anti-HLA-A2 (BB7.2) to assess the crossreactivity of the Ob.1A12 TCR in Jurkat cells stained with peptide/MHC tetramers and analyzed by flow cytometry. The Ob.1A12 TCR recognized multiple self-peptides presented by HLA-DR, with a single HLA residue playing a dominant role in this crossreactivity. In vivo experiments in HLA-DR-transgenic mice confirmed the autoimmune potential of the Ob.1A12 TCR, with TCR-transduced Jurkat cells co-cultured with antigen-presenting cells to measure activation.”
2. Self-reactive human CD4 T cell clones form unusual immunological synapses.
Singh, R., et al.J Exp Med. 2012 Feb 13;209(2):335-52. doi: 10.1084/jem.20112027. PMID: 22291028
“The study used anti-HLA-DR/DQ antibody (9.3F10) and anti-HLA-DR (L243) to block MHC class II and investigate synapse formation in self-reactive CD4 T cell clones in a humanized mouse model. Two-photon microscopy visualized synapse formation in lymph nodes, showing that 9.3F10 significantly reduced synapse stability and T cell activation. T cell receptor signaling was assessed by measuring calcium flux upon antigen recognition.”
3. Interleukin 2-Antibody and Tumor Necrosis Factor-Antibody Fusion Proteins Induce Different Antitumor Immune Responses In Vivo.
Christ, O., et al.Clin Cancer Res. 2001 May;7(5):1385-97. PMID: 11350943
“The study employed anti-HLA-DR/DQ antibody (clone 9.3F10), anti-CD80 (clone BB1), and anti-CD86 (clone IT2.2) for blocking experiments in SCID mice bearing human tumor xenografts. IL-2 and TNF fusion proteins were administered intravenously, with tumor growth monitored over 21 days. Flow cytometry confirmed HLA-DR/DQ expression on tumor cells, and the combination of IL-2 and TNF fusion proteins enhanced CD8+ T cell infiltration into tumors.”
4. CD4-Positive and CD8-Positive Cytotoxic T Lymphocytes Contribute to the Control of Cryptococcus neoformans in the Lungs of Mice.
Kawakami, H., et al.Clin Diagn Lab Immunol. 1999 Mar;6(2):236-41. PMID: 10092809
“C57BL/6 mice were injected intraperitoneally with 0.5 mg of anti-HLA-DR/DQ mAb (9.3F10) 1 day before and 2 days after Cryptococcus neoformans infection. The role of CD4+ and CD8+ T cells in controlling pulmonary infection was assessed, with flow cytometry quantifying T cell populations in lung tissues. Depletion of CD4+ or CD8+ T cells increased fungal burden, demonstrating their contribution to host defense against Cryptococcus neoformans.”
5. Human Mycobacterium tuberculosis-reactive CD4+ T-cell clones from healthy, latently infected individuals.
Demkowicz, A., et al.Infect Immun. 1991 Aug;59(8):2737-43. doi: 10.1128/iai.59.8.2737-2743.1991. PMID: 1879230
“The study used 25 microg/mL of anti-HLA-DR/DQ antibody (9.3F10) or anti-HLA class I (W6/32) to preincubate B cells, confirming MHC class II-restricted antigen presentation. CD4+ T cell clones from healthy individuals with latent Mycobacterium tuberculosis infection were isolated, and proliferation assays verified their specificity to mycobacterial antigens. Flow cytometry confirmed MHC class II expression on antigen-presenting B cells, establishing that CD4+ T cell responses were restricted by HLA-DR/DQ molecules.”
For more technical references or data sheets regarding the Recombinant Anti-Human HLA-DR/DQ Monoclonal Antibody (Clone: 9.3F10) formats, please contact our scientific support team at message@sydlabs.com.
Related Recombinant IgG Reference Antibodies:
Recombinant mouse IgG2a isotype control antibody, In vivo grade
Syd Labs provides the following in vivo grade recombinant anti-human HLA monoclonal antibodies:
Recombinant anti-human HLA-ABC monoclonal antibodies (clone W6/32), In vivo grade
Recombinant anti-human HLA-DR monoclonal antibodies (clone L243), In vivo grade
Recombinant anti-human HLA-DR/DP/DQ monoclonal antibodies (clone F3.3), In vivo grade
Syd Labs provides the following recombinant anti-human HLA monoclonal antibodies for flow cytometry:
Recombinant anti-human HLA-ABC monoclonal antibodies (clone W6/32) for flow cytometry
Recombinant anti-human HLA-DR monoclonal antibodies (clone L243) for flow cytometry
Recombinant anti-human HLA-DR/DP/DQ monoclonal antibodies (clone F3.3) for flow cytometry
Anti-Human HLA class II DR/DQ (9.3F10) from: Recombinant Anti-Human HLA class II DR/DQ Monoclonal Antibody, Mouse IgG2a Kappa (Clone: 9.3F10): PA007478 Syd Labs
