Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa PA007387.m2a Ushelf

Anti-human CD3e Antibody (Clone: 12F6) | PA007387.m2a

$150.00 – $900.00

Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007387.m2a
Product Name	In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 3, CD3D, CD3E, CD3G
Clone	12F6.
Isotype	Mouse IgG2a kappa.
Source/Host	The anti-human CD3e monoclonal antibody (clone: 12F6) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: 12F6) specifically binds to human CD3e.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD3e protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007387.m2a: Recombinant Anti-human CD3e Monoclonal Antibody (Clone: 12F6), Mouse IgG2a Kappa, In vivo Grade

Recombinant mouse IgG2a Monoclonal Antibody.

Background

The 12F6 antibody competes with the OKT3 antibody for binding to human T cells, and has more effective T-cell suppression and activation properties than OKT3. The 12F6 antibody is used to study T-cell functions and to manipulate T-cell activation and suppression, prevent acute allograft rejection, and treat autoimmune diabetes.

References of Anti-Human CD3e Monoclonal Antibody (Clone: 12F6)

1. Bispecific antibodies cross-link and redirect CD8+ T cells to kill cytomegalovirus-infected cells
Ali, A., et al.Sci Adv. 2024 Mar 1;10(9):eady2092. doi: 10.1126/sciadv.ady2092. PMID: 38442054
“A bispecific antibody utilizing the anti-CD3 antibody (clone 12F6) was constructed to engage T cells in NOD/SCID mice infected with CMV. In vivo studies demonstrated a significant reduction in viral load in treated mice. Flow cytometry confirmed CD8+ T cell activation in spleen and liver tissues, showing enhanced T cell-mediated cytotoxicity against CMV-infected cells. Control groups receiving isotype antibodies exhibited no significant viral clearance.”

2. Novel humanized anti-CD3 antibodies induce a predominantly immunoregulatory profile in human peripheral blood mononuclear cells
Li, J., et al.Immunol Lett. 2009 Aug 30;125(2):75-84. doi: 10.1016/j.imlet.2009.04.002. PMID: 19410632
“The humanized anti-CD3 antibody (12F6) was administered to humanized mice to evaluate its immunoregulatory effects. Treatment with 12F6 significantly reduced T cell proliferation in peripheral blood and increased IL-10 while decreasing IFN-γ levels, as shown by cytokine analysis. Flow cytometry on blood samples confirmed in vivo T cell depletion, demonstrating that 12F6 (anti-human CD3e antibody) induces a regulatory T cell phenotype.”

3. Phase I trial of a humanized, Fc receptor nonbinding anti-CD3 antibody, hu12F6mu in patients receiving renal allografts
Sandborn, W. J., et al.MAbs. 2010 Jul-Aug;2(4):444-55. doi: 10.4161/mabs.2.4.12305. PMID: 20564327
“The hu12F6mu antibody, a humanized version of the 12F6 antibody (anti-human CD3e antibody), was administered to renal allograft patients to prevent rejection. Flow cytometry confirmed reduced CD3+ T cell counts in peripheral blood within 48 hours. The trial assessed the safety profile of hu12F6mu in a dose-escalation study, with no significant cytokine release syndrome reported in the low-dose cohort.”

4. Construction and characterization of a humanized anti-human CD3 monoclonal antibody 12F6 with effective immunoregulation functions
Li, B., et al.Immunology. 2005 Dec;116(4):487-98. doi: 10.1111/j.1365-2567.2005.02249.x. PMID: 16014509
“A humanized anti-CD3 monoclonal antibody (12F6) was tested in NOD/SCID mice reconstituted with human PBMCs. The 12F6 antibody (anti-human CD3e antibody) effectively suppressed T cell-mediated graft-versus-host disease in vivo. Flow cytometry showed a significant reduction in CD3+ T cells in spleen samples and a shift toward regulatory T cell populations, with no significant cytokine release observed.”

5. Humanization of the murine anti-human CD3 monoclonal antibody OKT3
Adair, J. R., et al.Hum Antibodies Hybridomas. 1994;5(1-2):41-7. PMID: 8006592
“The 12F6 antibody (anti-human CD3e antibody) was used as a comparator to assess the efficacy of humanized OKT3 in a humanized mouse model. Both humanized OKT3 and 12F6 were administered to evaluate T cell suppression, showing comparable CD3+ T cell depletion via flow cytometry. In vivo experiments demonstrated reduced graft rejection in treated animals, confirming the immunosuppressive properties of both antibodies.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD3e Monoclonal Antibody (Clone: 12F6) formats, or related products from Syd Labs, please contact our scientific support team at message@sydlabs.com.