Anti-human CD4 Antibody (Clone: 13B8.2) | PA007385.m1

Catalog No.	PA007385.m1
Product Name	In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 4, leu-3, T4
Clone	13B8.2.
Isotype	Mouse IgG1 kappa.
Source/Host	The anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: 13B8.2) specifically binds to human CD4.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD4 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007385.m1: Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa, In vivo Grade

Recombinant mouse IgG1 Monoclonal Antibody.

Background

The recombinant 13B8.2 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

References of Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2)

1. Functionally specialized human CD4+ T-cell subsets express physicochemically distinct TCRs
Silvin, A., et al.Elife. 2020; 9:e57063. doi: 10.7554/eLife.57063. PMID: 33289628
“Peripheral blood mononuclear cells (PBMCs) from 12 donors were stained with anti-CD4–PE (clone 13B8.2) antibody, along with anti-CD27–PE-Cy5 (clone O323), anti-CD25–eFluor450 (clone BC96), anti-CD31–PE-Cy7 (clone WM59), and anti-CD45RA–FITC (clone JS-83) to identify recent thymic emigrants (RTEs), mature naive T cells, and naive Tregs in the CD4+ lineage. The study explored the plasticity of Th2, Th1, Th17, and Treg cells, noting that Th2 cells can adopt a Th1-like phenotype, while Th1 and Th17 cells can transition to Th2 in helminth-infected mice, and Th17 cells can acquire regulatory features like IL-10 production under IL-12 or IL-27 influence.”

2. Macrophage infection via selective capture of HIV-1-infected CD4+ T cells
Baxter, A. E., et al.Cell Host Microbe. 2014 Dec 10;16(6):711-21. doi: 10.1016/j.chom.2014.10.010. PMID: 25467409
“The study used the 13B8.2 antibody (anti-human CD4, 10 µg/ml) as an inhibitor of HIV-1 Env-receptor interactions, alongside soluble CD4, Tak-779, 2G12, and T20. The results suggest that macrophage uptake of HIV-1-infected CD4+ T cells contributes to the macrophage reservoir and viral dissemination in vivo, particularly after transmission or during massive CD4+ T cell death in lymphoid tissues. Detection of viral DNA or proteins in phagocytes may indicate uptake of infected immune cells rather than direct infection.”

3. In vitro antitumoral activity of baculovirus-expressed chimeric recombinant anti-CD4 antibody 13B8.2 on T-cell lymphomas
Troadec, S., et al.J Immunother. 2007 Feb-Mar;30(2):190-202. doi: 10.1097/01.cji.0000210069.83727.91. PMID: 17273799
“A baculovirus-expressed chimeric recombinant IgG1 antibody (rIgG1) with human Cγ1 and Cκ domains, derived from the murine anti-CD4 monoclonal antibody 13B8.2, was tested for antitumoral activity against CD4+ T-cell lymphomas. The 13B8.2 antibody, specific for the CDR3-like loop of CD4, inhibited HIV-1 replication and T cell proliferation in vitro and showed promising results in CD4+ lymphoma cell lines, leading to plans for in vivo preclinical studies in a murine xenograft model.”

4. Molecular and cellular analysis of human immunodeficiency virus-induced apoptosis in lymphoblastoid T-cell-line-expressing wild-type and mutated CD4 receptors
Guillerm, C., et al.J Virol. 1998 Oct;72(10):8061-72. doi: 10.1128/JVI.72.10.8061-8072.1998. PMID: 9733846
“The 13B8.2 monoclonal antibody, targeting the CD4 region critical for receptor dimerization, prevented apoptosis in HIV-infected cells without inhibiting viral replication. The study highlighted high levels of HIV replication and immune system activation in vivo, contributing to enhanced apoptosis in both infected and bystander CD4 cells. Fas signaling was noted as a potential contributor to T-cell depletion in HIV-1-infected patients.”

5. Differential CD4-dependent inhibition of JNK but not Erk-2 activities by anti-CD4 monoclonal antibodies
Chene, C., et al.Int Immunol. 1998 Jul;10(7):869-78. doi: 10.1093/intimm/10.7.869. PMID: 9701025
“The study observed differential inhibition of JNK but not Erk-2 activities by the anti-CD4 monoclonal antibody 13B8.2 in Jurkat T cells. In vivo experiments in mouse models showed that administration of 13B8.2 led to selective inhibition of JNK activity in T cells, demonstrating that CD4 ligation modulates T cell activation through differential kinase inhibition.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry

Anti-human CD4 (13B8.2) from: Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2): PA007385.m1 Syd Labs