Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Mouse IgG2a Kappa PA007466.m2a Syd Labs

Anti-human CD20 Antibody for Flow Cytometry | PA007466.m2a

$100.00 – $200.00

Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007466.m2a
Product Name	Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9), Mouse IgG2a Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD antigen CD20, CD20
Clone	B9E9.
Isotype	Mouse IgG2a Kappa.
Source/Host	The anti-human CD20 monoclonal antibody (clone: B9E9) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant B9E9 antibody for Flow Cytometry specifically binds to mouse CD20.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9) for Flow Cytometry, Mouse IgG2a Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007466.m2a: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: B9E9) for Flow Cytometry, Mouse IgG2a Kappa, In vivo Grade

References of Anti-Human CD20 Monoclonal Antibody (Clone: B9E9)

1. Induction of viral and tumour specific CTL responses using antibody targeted HLA class I peptide complexes
Savage, P., et al.Br J Cancer. 2002 Apr 22;86(8):1336-42. doi: 10.1038/sj.bjc.6600223. PMID: 11953895
“The B9E9 scFvSA fusion protein, containing the single-chain variable region of the murine IgG2a anti-CD20 antibody B9E9 fused to streptavidin, was used to deliver HLA-A2/class I peptide complexes to HLA class I-negative Daudi cells. PBMCs were incubated with B9E9 scFvSA (10 μg/ml) for 1 hour, and flow cytometry with antibodies like anti-MHC class I (W6/32), anti-CD19, anti-CD80, and anti-CD86 confirmed binding and CTL induction.”

2. A tetravalent single-chain antibody-streptavidin fusion protein for pretargeted lymphoma therapy
Schultz, J., et al.Cancer Res. 2000 Dec 1;60(23):6663-9. PMID: 11118050
“The B9E9 scFvSA fusion protein, derived from the CD20-specific murine IgG2a monoclonal antibody B9E9, was genetically engineered for pretargeted radioimmunotherapy. The V H and V L DNA fragments were amplified from B9E9 hybridoma cells, and the resulting scFvSA fusion protein showed nanomolar affinity to CD20-positive Ramos cells, comparable to the original B9E9 monoclonal antibody, as confirmed by in vitro and in vivo characterization.”

For more technical references or data sheets regarding the Anti-Human CD20 Monoclonal Antibody (Clone: B9E9) formats, please contact our scientific support team at message@sydlabs.com.