Description

PA007455: Recombinant Anti-Human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa, In vivo Grade

The in vivo grade recombinant anti-human CD20 Mouse IgG2b Kappa monoclonal antibody was produced in mammalian cells.

References of Anti-Human CD20 Monoclonal Antibody (Clone: 2H7)

1. Combination treatment with anti-CD20 and oral anti-CD3 prevents and reverses autoimmune diabetes.
Hu, C., et al.Diabetes. 2013 Aug;62(8):2849-58. doi: 10.2337/db12-1175. PMID: 23447122
“Thus, the findings demonstrate that combining anti-CD20 and oral anti-CD3 is superior to anti-CD20 monotherapy for restoring normoglycemia in diabetic NOD mice, providing important preclinical evidence for the optimization of B cell-directed therapy for T1D. …Thus, we tested the effect of intravenous anti-CD20 and oral anti-CD3 combined treatment for the prevention and reversal of T1D in the human CD20 transgenic NOD (hCD20/NOD) animal model. …Herein, we show that oral administration of anti-CD3 together with intravenous injection of anti-CD20 has a synergistic effect on the prevention and reversal of T1D in the hCD20/NOD mouse. …All fluorochrome-conjugated monoclonal antibodies (mAbs) were purchased from BioLegend, Inc. Affinity-purified mouse anti-hCD20 mAb 2H7 was prepared by Bio X Cell. …To further improve the efficacy of treatment, we tested the combination treatment of anti-hCD20/oral anti-CD3 in hCD20/NOD mice.”

2. B-cell depletion reactivates B lymphopoiesis in the BM and rejuvenates the B lineage in aging.
Keren, Z., et al.Blood. 2011 Mar 17;117(11):3104-12. doi: 10.1182/blood-2010-09-307983. PMID: 21228330
“Mice used were C57Bl6 mice that are normal, or carrying a targeted conditional Baff-r locus (BAFF-RFL),25 transgenic for Mx-cre,26 R26R-EYFP transgene for the EYFP-Cre reporter system,27 or Balb/c transgenic for human CD20 (hCD20 Tg). …An “old-like” B-cell phenotype in C57Bl6 mice and the hCD20Tg mice was determined when the frequency of newly generated AA4.1-expressing B cells in peripheral blood was less than 5% of the B220+ cells (supplemental Figure 4, available on the Blood Web site; see the Supplemental Materials link at the top of the online article). …To deplete B cells in the hCD20 Tg model, mice were injected intraperitoneally with 1 mg/mouse of mouse anti-human CD20 monoclonal antibodies (clone 2H7) as described. …To do so, we used transgenic mice expressing human CD20 specifically in the B lineage (hCD20 Tg mice), starting from the late pre-B stage. …After B-cell depletion, complete B-cell reconstitution in hCD20 Tg mice lasted 60 to 70 days, as described.”

3. Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure.
Polyak, M. J., et al.Blood. 2002 May 1;99(9):3256-62. doi: 10.1182/blood.v99.9.3256. PMID: 11964291
“CD20, a nonglycosylated 33- to 35-kd integral membrane protein expressed at high density only on B lymphocytes, has proven to be an effective target for immunotherapeutic removal of malignant B cells. …It is generally assumed, based on antibody blocking studies,5 that CD20 bears only 2 extracellular epitopes, one that is recognized by the vast majority of CD20 mAbs, and a second that is recognized by a single antibody (1F5) with unusual activating properties. …Other CD20 mAbs were obtained from the human leukocyte differentiation workshops IV, VI, and VII. …CD20 translocation experiments were performed as described.14 Briefly, cells were treated before lysis with anti-CD20 mAbs for 15 minutes at 37°C and both the cleared lysates and the insoluble pellets were collected for analysis by CD20 immunoblotting. …Equal aliquots of each fraction were mixed with SDS sample buffer. Western blot analysis and Coomassie staining were used to identify the fractions containing CD20 and the molecular weight standards, respectively.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD20 Monoclonal Antibody (Clone: 2H7) formats, or related products from Syd Labs, please contact our scientific support team at message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
Recombinant mouse IgG2a isotype control antibody, In vivo grade

Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies:
Recombinant anti-human CD20 antibodies (clone 2H7), In vivo grade
Recombinant anti-human CD20 antibodies (clone B9E9), In vivo grade

Anti-Human CD20 (2H7) from: Anti-Human CD20 Monoclonal Antibody, Mouse IgG2b Kappa (Clone: 2H7): PA007455 Syd Labs