Anti-Human CD28 Antibody (Clone: 9.3) | PA007458

Catalog No.	PA007458
Product Name	In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody, Mouse IgG2a Kappa (Clone: 9.3)
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	T-cell-specific surface glycoprotein CD28; TP44
Clone	9.3.
Isotype	Mouse IgG2a Kappa.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: 9.3) specifically binds to mouse CD28.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD28 protein.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The in vivo grade recombinant anti-human CD28 antibodies (clone of 9.3) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007458: Recombinant Anti-Human CD28 Monoclonal Antibody (Clone: 9.3), Mouse IgG2a Kappa , In vivo Grade

The in vivo grade recombinant anti-human CD28 Mouse IgG2a Kappa monoclonal antibody was produced in mammalian cells.

References of Anti-Human CD28 Monoclonal Antibody (Clone: 9.3)

1. Profiling T cell activation using single-molecule fluorescence in situ hybridization and flow cytometry.
Bushkin, Y., et al.J Immunol. 2015 Jan 15;194(2):836-41. doi: 10.4049/jimmunol.1401515. PMID: 25505292
“Flow cytometric characterization of Ag-specific T cells typically relies on detection of protein analytes. …Shifting the analysis to detection of RNA would provide several significant advantages, which we illustrate by developing a new host immunity-based platform for detection of infections. …Cytokine mRNAs synthesized in response to ex vivo stimulation with pathogen-specific Ags are detected in T cells with single-molecule fluorescence in situ hybridization followed by flow cytometry. …Background from pre-existing in vivo analytes is lower for RNAs than for proteins, allowing greater sensitivity for detection of low-frequency cells. …The utility of probing immunological memory of infections is demonstrated by detecting T cells that recognize mycobacterial and viral Ags in donors exposed to the respective pathogens.”

2. Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter.
Hill, E. V., et al.Eur J Immunol. 2015 Apr;45(4):1103-15. doi: 10.1002/eji.201444661. PMID: 25627813
“To investigate the requirement for APCs during GSK3 inhibition mediated induction of IL-10 in CD4+ T cells, naive or polarized Tg4 CD4+ T cells were cultured with anti-CD3/ anti-CD28 stimulation in the presence of GSK3 inhibitors and IL-10 secretion was assessed. …(A) Naive CD4+ T cells were isolated from Tg4 splenocytes and cultured with anti-CD3 and anti-CD28 in the presence of IL-2 and GSK3 inhibitors or vehicle control. …NS, not significant (ANOVA). (B) Th1 polarized Tg4 cells were restimulated with anti-CD3 and anti-CD28 in the presence of IL-2 and GSK3 inhibitors or vehicle control. …Alternatively, live CD4+ T cells were isolated by Ficoll centrifugation and then magnetic separation using the CD4+ T cell Isolation kit II and restimulated with either anti-CD3 and anti-CD28 coated beads (Dynabeads, Life Technologies) or on anti-CD3 (1 µg/mL, eBioscience; clone 145-2C11) and anti-CD28 (2 µg/mL, eBioscience; clone 37.51) coated plates. …Total mRNA from 3-5 × 10^6 CD4+ T cells after overnight anti-CD3 and anti-CD28 stimulation was isolated using RNAeasy Mini Kit (Qiagen) and quality and quantity were verified using a Nanodrop (Thermo scientific).”

For more technical references or data sheets regarding the Recombinant Anti-Human CD28 Monoclonal Antibody (Clone: 9.3) formats, please contact our scientific support team at message@sydlabs.com.

Related Recombinant IgG Reference Antibodies:
Recombinant mouse IgG2a isotype control antibody, In vivo grade

Syd Labs provides the following recombinant anti-human CD28 monoclonal antibodies:
Recombinant anti-human CD28 antibodies (clone 15E8), In vivo grade
Recombinant anti-human CD28 antibodies (clone 9.3), In vivo grade
Recombinant anti-human CD28 antibodies (clone CD28.3), In vivo grade

Syd Labs provides the following recombinant anti-mouse CD28 monoclonal antibodies:
Recombinant anti-mouse CD28 antibodies (clone PV-1), In vivo grade

Anti-Human CD28 (9.3) from: In vivo Grade Recombinant Anti-Human CD28 Monoclonal Antibody : PA007458 Syd Labs