Recombinant Mouse IgG Antibody Mix with more mIgG2b and mIgG2c rather than mIgG2a

Catalog No.	PA007220
Product Name	Recombinant Mouse IgG Antibody Mix with more mIgG2b and mIgG2c rather than mIgG2a
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	HyHEL-10.
Isotype	mouse IgG1, IgG2b, IgG2c, and IgG3 kappa.
Specificity/Sensitivity	HEL-specific; neither human nor mouse samples.
Applications	ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, and isotype controls.
Reactivity	None of Human, Mouse, and Rat.
Purification	The recombinant mouse IgG antibodies were affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A and G.
Form Of Antibody	1x PBS.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Mouse IgG Antibody Mix with more mIgG2b and mIgG2c rather than mIgG2a is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. 1 month from date of receipt, 2 to 8°C as supplied. 12 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant mouse IgG antibody mixes: animal free production; lot-to-lot consistency; no binding to human tissues; adjustable formulation of the mouse IgG antibodies in the antibody mixes. Best normal mouse serum alternative; normal mouse serum blocking reagent alternative; serum-derived mouse IgGs alternative.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card.

Description

PA007220: Recombinant Mouse IgG Antibody Mix with more mIgG2b and mIgG2c rather than mIgG2a

The recombinant mouse IgG antibody mix is a mixture of recombinant anti-HEL mouse IgG1, IgG2b, IgG2c, and IgG3 kappa antibodies which bind to neither human nor mouse samples. The percentage of mouse IgG2b is much higher than the other antibody subclasses in the antibody mix.

Background of Recombinant Mouse IgG Antibody Mix with more mIgG2b and mIgG2c rather than mIgG2a

Normal mouse serum and purified mouse IgGs from normal mouse serum are commonly used as non-specific passive blocking solution for antibody-based immuno-assays. Recombinant mouse IgG antibody mixes, as a direct alternative to normal mouse serum and even mouse IgGs purified from mouse serum or a non-specific passive blocking solution, can be used to eliminate interfering antibodies from immuno-assay systems using capture and detection antibodies. There is higher percentage of mouse IgG1 or IgG2b in serum from different strains of mice. So, recombinant mouse IgG antibody mixes with different percentages of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 were made to meet customers’ requirements of different applications. A customized recombinant mouse IgG antibody mix with different percentages of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 can be provided.

Features of recombinant mouse IgG antibody mixes compared with normal mouse serum and serum-derived mouse IgGs:

1) Animal free production. CHO cells rather than mice are used in antibody production.

2) Lot-to-lot consistency. CHO cells which are used for antibody drug manufacturing are used to manufacture the mouse IgG antibodies in the antibody mixes. All production is under control and predictable.

3) As a targeted or passive antibody blocker to interfering antibodies like HAMA (Human Anti-Mouse Antibodies) and no binding to human tissues. In contrast, some serum-derived mouse IgGs may bind to unknown targets in some human tissues.

4) Adjustable percentages of the antibodies in the antibody mixes. A customized recombinant mouse IgG antibody mix with different percentages of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 can be easily made according to customers’ applications.

Recombinant mouse IgG antibody mixes are the best normal mouse serum alternative as blocking reagents, and serum-derived mouse IgGs alternative.

Questions and Answers:

Question: Normal mouse serum is often used for blocking or saturating immunoassays, especially those involving tissue samples such as immunohistochemistry (IHC). How much should recombinant antibody mixes be added to replace normal mouse serum?
Answer: Our recombinant mouse IgG antibody mixes can be used as normal mouse serum alternative for blocking reagent, and more specifically, the purified normal IgGs alternative. It was said that there is 80 to 90 mg/ml protein in a normal mouse serum sample, and 7 to 10 mg/ml IgG in a typical mouse serum and plasma sample. It means that there is 7.8% to 12.5% IgGs in proteins in normal mouse serum. One may calculate the amount of mouse IgGs in a mouse serum sample. One should also consider the percentage of various mouse IgG isotypes in the mouse serum sample vs recombinant mouse IgG antibody mixes. Conditions of optimal mouse IgG antibody mix performance should be determined experimentally by customers.

The most popular human Fc blocking reagents include:
Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls.
Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions.

Best choice of human Fc blocking reagents based on your application are:
For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

Syd Labs provides the following anti-human Fc blocking reagents:

Anti-human CD16 monoclonal antibody (Clone: 3G8)
Anti-human CD32 monoclonal antibody (Clone: IV.3)
Anti-human CD64 monoclonal antibody (Clone: 22)
Purified human IgG
Recombinant human IgG antibody mixes
Recombinant human IgG isotype control antibodies
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies

Syd Labs provides the following anti-mouse Fc blocking reagents:

Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies

Please remember our product information: Recombinant Mouse IgG Antibody Mix with more mIgG2b and mIgG2c rather than mIgG2a: PA007220 Syd Labs

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