Purified Human IgG (Whole Molecule), Native
$150.00 – $1,500.00
Purified human IgG (whole molecule) from a mixture of normal human serum. A human Fc blocking reagent or a component of a human Fc blocking solution. Recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are available.
- Details & Specifications
- References
- More Offers
| Catalog No. | PA007395 |
|---|---|
| Product Name | Purified Human IgG (Whole Molecule), Native |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | Human Immunoglobulins, Human Fc Blocking Reagent |
| Summary | The purified IgG polyclonal antibody (whole molecule) was purified from a mixture of normal human serum. |
| Clone | N/A |
| Isotype | Human IgG |
| Specificity/Sensitivity | N/A |
| Applications | ELISA, multiplex and microarray assays, and human Fc blocking reagent for immunoassays including flow cytometry, IHC, IF, WB, and ELISA. |
| Form Of Antibody | Lyophilized, no stabilizers or preservatives. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 month from date of receipt, 2 to 8°C as supplied. |
| Note | Purified human IgG (whole molecule) from a mixture of normal human serum. A human Fc blocking reagent or a component of a human Fc blocking solution. Recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are available. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Orleave a message with a formal purchase order (PO) Or credit card. |
Description
PA007395: Purified Human IgG (Whole Molecule), Native.
Native human IgG Polyclonal Antibody (whole molecule).
Background
The purified native human IgG (whole molecule) from a mixture of normal human serum can be used as a human Fc blocking reagent or a component of a human Fc blocking solution.
The most popular human Fc blocking reagents include:
Anti-human CD16 (clone 3G8), and CD32 (Clone IV.3): for flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for FcγRII (CD32) and FcγRIII (CD16), and reduces background staining; it may not block all Fc receptors. Clones 10.1, and 22 or H22 are the most widely used anti-human FcγRI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Purified human IgGs or mixes of human isotype controls: for general Fc blocking in IHC and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Syd Labs supply various human IgG isotype controls.
Normal mouse serum, purified mouse IgG or isotype controls: for flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Bovine Serum Albumin (BSA) or FBS: for general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: for flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions.
Best choice of human Fc blocking reagents based on your application are:
For flow cytometry: anti-human CD16 (Clone 3G8), CD32 (Clone IV.3), and CD64 (Clone 10.1, and 22 or H22).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or human IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant human IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.
Syd Labs provides the following anti-human Fc blocking reagents:
Anti-human CD16 monoclonal antibody (Clone: 3G8)
Anti-human CD32 monoclonal antibody (Clone: IV.3)
Anti-human CD64 monoclonal antibody (Clone: 22)
Purified human IgG
Recombinant human IgG antibody mixes
Recombinant human IgG isotype control antibodies
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies
Syd Labs provides the following anti-mouse Fc blocking reagents:
Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)
Recombinant mouse IgG antibody mixes
Recombinant mouse IgG isotype control antibodies
Recombinant rat IgG antibody mixes
Recombinant rat IgG isotype control antibodies
Please remember our product information: Purified Human IgG (Whole Molecule), Native: PA007395 Syd Labs