Anti-human CD19 Antibody for Flow Cytometry | PA007423.m2a

Catalog No.	PA007423.m2a
Product Name	Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	B-lymphocyte antigen CD19, Cluster of Differentiation 19, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, CVID3
Clone	B43.
Isotype	Mouse IgG2a kappa.
Source/Host	The recombinant anti-human CD19 monoclonal antibody (clone: B43) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant B43 antibody for Flow Cytometry specifically binds to the human CD19 protein.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43) for Flow Cytometry, Mouse IgG2a Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007423.m2a: Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43) for Flow Cytometry, Mouse IgG2a Kappa, In vivo Grade

The B43 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of Anti-Human CD19 Monoclonal Antibody (Clone: B43)

1. Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins
Caron, A. W., et al.BMC Biotechnol. 2009 May;9(42):42. PMID: 19432976
“The light chain of the B43 antibody (anti-human CD19) was replaced in the pMPGB43k expression vector, which carries an enhanced CMV enhancer/promoter and a hygromycin resistance gene, to construct a new vector for recombinant protein expression. The B43 antibody (anti-human CD19 mAb) coding sequence was modified via PCR and cloned into the PmeI site of pMPGB34k, with a BamHI site added for further modifications. The study also isolated the IGF-1 coding sequence from murine cDNA for constructing pMPG-IGF-E5.”

2. Downregulation of major histocompatibility complex class I molecules by Kaposi’s sarcoma-associated herpesvirus K3 and K5 proteins
Ishido, S., et al.J Virol. 2000 Jun;74(11):5300-9. PMID: 10799607
“The B43 antibody (anti-human CD19) was used alongside W6/32 (MHC class I), RPA-T8 (CD8), M-A712 (transferrin receptor), and TU39 (MHC class II) for fluorescence-activated cell sorting and confocal immunofluorescence analyses. K3 and K5 proteins specifically downregulated MHC class I without affecting MHC class II, immunoglobulin M, or CD19 surface levels, as confirmed by these antibodies.”

3. Large scale manufacturing of B43(anti-CD19)-genistein for clinical trials in leukemia and lymphoma
Myers, D. E., et al.Leuk Lymphoma. 1998 Apr;29(3-4):329-38. PMID: 9684930
“The B43(anti-human CD19 antibody)-genistein immunoconjugate was manufactured at large scale for clinical trials in leukemia and lymphoma. The B43 antibody was purified from ascites fluid using protein A affinity chromatography, and its conjugation to genistein was optimized for high yield. Flow cytometry confirmed effective binding of B43-genistein to CD19+ leukemic cells, with cytotoxicity tested against leukemia cell lines.”

4. Pharmacokinetic features, immunogenicity, and toxicity of B43(anti-CD19)-pokeweed antiviral protein immunotoxin in cynomolgus monkeys
Kreitman, R. J., et al.Clin Cancer Res. 1997 Mar;3(3):325-37. PMID: 9815689
“The B43(anti-human CD19)-pokeweed antiviral protein (PAP) immunotoxin was evaluated in cynomolgus monkeys for pharmacokinetics, immunogenicity, and toxicity. B43-PAP, constructed by linking the anti-CD19 monoclonal antibody B43 to PAP, killed >99.9% of primary leukemic progenitor cells from B-lineage ALL patients. The study revealed dose-limiting renal and cardiac toxicity in mice and severe renal tubular necrosis in monkeys.”

5. Membrane-associated CD19-LYN complex is an endogenous p53-independent and Bc1-2-independent regulator of apoptosis in human B-lineage lymphoma cells
Myers, D. E., et al.Proc Natl Acad Sci U S A. 1995 Oct;92(21):9575-9. PMID: 7568175
“The B43 monoclonal antibody (anti-human CD19 mAb) was used to cross-link membrane-associated CD19, activating the LYN kinase signaling pathway and inducing apoptosis in B-lineage lymphoma cells in a p53- and Bcl-2-independent manner. The CD19-LYN complex was immunoprecipitated using B43 antibody, and flow cytometry confirmed high-level CD19 expression on lymphoma cells.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD19 Monoclonal Antibody (Clone: B43) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:

Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa
Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: B43), Human IgG1 Fc Silent Kappa

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:

Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:

Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:

Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)

Anti-human CD19 (B43) for Flow Cytometry from: Anti-human CD19 Monoclonal Antibody (Clone: B43), Mouse IgG2a Kappa: PA007423.m2a Syd Labs