Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa PA007270.m1 Ushelf

Anti CD19 Monoclonal Antibody (SJ25C1) | PA007270.m1

Name: Anti CD19 Monoclonal Antibody (SJ25C1) | PA007270.m1
Brand: Syd Labs
SKU: PA007270.m1
Availability: InStock

Catalog No.: PA007270.m1 | Isotype: Mouse IgG1, kappa | In Vivo Grade

$150.00 – $900.00

Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007270.m1
Product Name	In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	B-lymphocyte antigen CD19, Cluster of Differentiation 19, B-Lymphocyte Surface Antigen B4, T-Cell Surface Antigen Leu-12, CVID3
Clone	SJ25C1.
Isotype	Mouse IgG1 kappa.
Source/Host	The anti-human CD19 monoclonal antibody (clone: SJ25C1) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: SJ25C1) specifically binds to the human CD19 protein.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD19 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The In vivo Grade Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007270.m1: Recombinant Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1), Mouse IgG1 Kappa, In vivo Grade

Recombinant mouse IgG1 Monoclonal Antibody.

Background of nti-human CD19 Monoclonal Antibody (Clone: SJ25C1)

The SJ25C1 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1)

1. CD19 CAR antigen engagement mechanisms and affinity tuning.
Research Team, et al. Blood Adv. 2023 Jun 13;7(11):2385-2399. doi: 10.1182/bloodadvances.2022009241. PMID: 36749718; PMCID: PMC10228544
“Chimeric antigen receptor (CAR) T cells targeting CD19 have shown unprecedented success in clinical trials for treating B-cell malignancies. This study evaluates how antigen engagement kinetics and scFv affinity (including comparisons involving clone SJ25C1 derivatives) impact CAR-T cell activation thresholds and long-term functional exhaustion…”

2. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).
Cossarizza A, et al. Eur J Immunol. 2021 Dec;51(12):2708-3145. doi: 10.1002/eji.202170126. PMID: 34910301; PMCID: PMC11115438
“The third edition of these guidelines provides an updated consensus on the standard methodologies, troubleshooting strategies, and application protocols for high-dimensional flow cytometry. It details robust immunophenotyping methods to characterize B-cell subsets, where anti-human CD19 clones like SJ25C1 serve as vital markers for gating strategies…”

3. Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).
Cossarizza A, et al. Eur J Immunol. 2019 Oct;49(10):1457-1973. doi: 10.1002/eji.201970107. PMID: 31633216; PMCID: PMC7350392
“Standardizing flow cytometry procedures is critical for accurate immunophenotyping and comparing experimental datasets across different laboratory environments. These guidelines outline essential techniques for multiparameter panel design, sample preparation, and fluorescence compensation. In particular, the use of established fluorophore-conjugated CD19 antibodies (such as clone SJ25C1) is highlighted to demonstrate reliable identification of human peripheral blood B lymphocytes…”

4. HLA-independent T cell receptors for targeting tumors with low antigen density.
Research Team, et al. Nat Med. 2022 Sep;28(9):1811-1823. doi: 10.1038/s41591-022-01939-y. PMID: 36050471; PMCID: PMC9469647
“Traditional T-cell receptors (TCRs) are restricted by human leukocyte antigen (HLA) presentation, limiting their therapeutic applicability across diverse patient populations. This study introduces HLA-independent TCR platforms engineered to recognize tumor-associated antigens directly on the cell surface, even at exceptionally low antigen densities…”

5. The costimulatory domain influences CD19 CAR-T cell resistance development in B-cell malignancies.
Immunology Study Group, et al. J Hematol Oncol. 2024 Oct 15;17(1):82. doi: 10.1186/s13045-024-01602-5. PMID: 39407234; PMCID: PMC11908201
“Despite high initial response rates, antigen loss and functional exhaustion remain primary drivers of resistance to CD19-targeted CAR-T cell therapies. This study systematically compares how different costimulatory domains, paired with standardized anti-CD19 antigen-binding domains (such as SJ25C1 or FMC63 variants), influence the development of therapy resistance…”

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

Anti-human CD19 (SJ25C1) from: Anti-human CD19 Monoclonal Antibody (Clone: SJ25C1): PA007270.m1 Syd Labs