In vivo Grade Recombinant Anti-mouse CD3 Rat IgG2b Kappa Monoclonal Antibody (Clone: 17A2)

Anti Mouse CD3 Antibody, clone 17A2 | PA007199.r2b

$150.00 – $900.00

Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007199.r2b
Product Name	In vivo Grade Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Rat IgG2b Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 3, CD3D, CD3E, CD3G.
Summary	The anti-mouse CD3 antibody (clone: 17A2) was produced in mammalian cells.
Clone	17A2
Isotype	Rat IgG2b kappa
Specificity/Sensitivity	The in vivo grade recombinant rat monoclonal antibody (clone: 17A2) specifically binds to the mouse T cell receptor CD3.
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD3 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The 17A2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007199.r2b: Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Rat IgG2b Kappa, In vivo Grade

In vivo Grade Recombinant Anti-mouse CD3 Rat IgG2b Kappa Monoclonal Antibody (Clone: 17A2) was produced in mammalian cells. The ‌Anti-Mouse CD3 Monoclonal Antibody (Clone 17A2)‌ is a high-affinity, rigorously validated reagent designed for precise detection and quantification of CD3ε, a critical pan-T cell marker in murine models. This antibody demonstrates exceptional specificity for mouse CD3, validated in applications including ‌flow cytometry (FACS)‌, ‌immunohistochemistry (IHC)‌, ‌immunofluorescence (IF)‌, and ‌Western blotting‌, ensuring reliable performance across diverse experimental setups. Clone 17A2 targets the ε-chain of the CD3 complex, enabling accurate identification of T cell populations in lymphoid tissues, peripheral blood, or cultured cells. Ideal for studies in immunology, autoimmune disorders, and oncology.

References of Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2)

1. CD3 aptamers promote expansion and persistence of tumor-reactive T cells for adoptive T cell therapy in cancer
Menon AP, et al. Mol Ther Nucleic Acids. 2024 May; 35(2):102198. doi: 10.1016/j.omtn.2024.102198. PMID: 38745854; PMCID: PMC11091522
“The CD3/T cell receptor (TCR) complex is responsible for antigen-specific pathogen recognition by T cells, and initiates the signaling cascade necessary for activation of effector functions. CD3 agonistic antibodies are commonly used to expand T lymphocytes in a wide range of clinical applications, including in adoptive T cell therapy for cancer patients. […] The aptamers improved efficacy and persistence of adoptive T cell therapy by low-affinity TCR-reactive T lymphocytes in melanoma-bearing mice.”

2. Murine RAW Macrophages Are a Suitable Model to Study the CD3 Signaling in Myeloid Cells
Castro K, et al. Cells. 2022 May 13; 11(10):1635. doi: 10.3390/cells11101635. PMID: 35626672; PMCID: PMC9139304
“In recent years, a growing body of evidence has shown the presence of a subpopulation of macrophages that express CD3, especially in the context of mycobacterial infections. […] This work aimed to evaluate the expression of CD3 in a murine macrophage cell line and its potential for the study of CD3 signaling. […] Our study provides evidence to support that RAW cells are a suitable model to study the function and signaling of the CD3 complex in myeloid cells.”

3. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza A, Chang HD, Radbruch A, et al. Eur J Immunol. 2021 Dec; 51(12):2708-3145. doi: 10.1002/eji.202170126. PMID: 34910301; PMCID: PMC11115438
“The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical diagnostics and specific subsets of immune cells.”

4. Rigid crosslinking of the CD3 complex leads to superior T cell stimulation
Nelson AD, Wang L, et al. Front Immunol. 2024; 15:1406456. PMID: 39281668; PMCID: PMC11392757
“Functionally bivalent non-covalent Fab dimers (Bi-Fabs) specific for the TCR/CD3 complex promote CD3 signaling on T cells. While comparing functional responses to stimulation with Bi-Fab, F(ab’)2 or mAb specific for the same CD3 epitope, we observed fratricide requiring anti-CD3 clustering. […] Thus, increasing rigidity of Fab-CD3 crosslinking between opposing effector-target pairs may result in stronger T cell effector function.”

5. Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival
Shirali AS, et al. J Clin Invest. 2018 Nov 1; 128(11):4943-4956. doi: 10.1172/JCI120923. PMID: 30277476; PMCID: PMC6205374
“The targeted delivery of therapeutic drugs to lymph nodes (LNs) provides an unprecedented opportunity to improve the outcomes of transplantation and immune-mediated diseases. […] Here, we have synthesized a nanoparticle (NP) as a carrier of anti-CD3, and optimized the conjugation strategy to coat the NP surface with MECA79 mAb (MECA79-anti-CD3-NP) to enhance LN accumulation. […] Short-term treatment of murine cardiac allograft recipients with MECA79-anti-CD3-NP resulted in significantly prolonged allograft survival in comparison with the control groups.”

Syd Labs provides the following in vivo grade recombinant anti-human CD3 monoclonal antibodies:
Muromonab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Teplizumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Foralumab biosimilar, research grade, anti-human CD3 monoclonal antibody (Clone: OKT3)
Anti-human CD3 monoclonal antibody (Clone: OKT3)
Anti-human CD3 monoclonal antibody (Clone: SP34-2)
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1)
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6)

Syd Labs provides the following in vivo grade recombinant anti-human CD3 bispecific antibodies:
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / UCHT1)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: OKT3 / SP34-2)
Recombinant Anti-human CD3 / CD3 Bispecific Antibody (Clone: SP34-2 / OKT3)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD3 monoclonal antibodies:
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11)
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2)
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2)

Syd Labs provides the following recombinant anti-human CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD3 monoclonal antibody (Clone: OKT3) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: UCHT1) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: 12F6) for flow cytometry
Recombinant Anti-human CD3 monoclonal antibody (Clone: SP34-2) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD3 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD3 monoclonal antibody (Clone: 17A2) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 145-2C11) for flow cytometry
Recombinant Anti-mouse CD3e monoclonal antibody (Clone: 500A2) for flow cytometry

Anti-mouse CD3 Antibody(17A2) from: In vivo Grade Recombinant Anti-mouse CD3 Rat IgG2b Kappa Monoclonal Antibody (Clone: 17A2): PA007199.r2b Syd Labs