Anti-human CD3e Antibody (Clone: UCHT1) | PA007197

$150.00 – $900.00

Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007197
Product Name	In vivo Grade Recombinant Anti-human CD3e Monoclonal Antibody, Mouse IgG1 Kappa (Clone: UCHT1)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 3, CD3D, CD3E, CD3G
Summary	The anti-human CD3 monoclonal antibody (clone: UCHT1) was produced in mammalian cells.
Clone	UCHT1
Isotype	Mouse IgG1 kappa
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: UCHT1) specifically binds to the human T cell receptor CD3.
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD3 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-human CD3 Mouse IgG1 Kappa monoclonal antibody (clone: UCHT1) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007197: Recombinant Anti-human CD3 Monoclonal Antibody (Clone: UCHT1), Mouse IgG1 Kappa, In vivo Grade

Recombinant mouse IgG1 Monoclonal Antibody.

References of Anti-Human CD3 Monoclonal Antibody (Clone: UCHT1)

1. In vivo DNA-launched bispecific T cell engager targeting IL-13Rα2 limits tumor growth in a humanized mouse model
Wei J, et al. Mol Ther Oncolytics 2022. doi: 10.1016/j.omto.2022.09.006
“This study evaluates a DNA-launched bispecific T cell engager (BiTE) targeting IL-13Rα2 in a humanized NSG mouse model engrafted with human PBMCs. Flow cytometry analysis utilized anti-human CD45 (HI30), anti-human CD3 (UCHT1), anti-human CD4 (OKT4) …… The IL-13Rα2 BiTE significantly reduced tumor growth compared to controls.”

2. SCENITH: A Flow Cytometry-Based Method to Functionally Profile Energy Metabolism with Single-Cell Resolution
Argüello RJ, et al. Cell Metab 2020. doi: 10.1016/j.cmet.2020.11.007
“This study introduces SCENITH, a flow cytometry-based method to profile energy metabolism at single-cell resolution. Immune cell phenotyping was performed using antibodies including anti-CD45 (HI30), anti-CD3 (UCHT1), anti-CD4 (RPA-T4) …… SCENITH measures protein synthesis rates as a proxy for cellular energy metabolism, validated in human immune cells.”

3. An IgG-based bispecific antibody for improved dual targeting in vivo
Kellner S, et al. EMBO Mol Med 2020. doi: 10.15252/emmm.201911902
“This study investigates an IgG-based bispecific antibody in NSG mice engrafted with human PBMCs and tumor cells. T cells were stained with anti-CD3 (UCHT1) and anti-CD8 (SK1) antibodies for flow cytometry analysis …… peripheral blood analyzed for T cell activation and cytokine release post-treatment.”

4. In vitro Characterization of the Anti-PD-1 Antibody Nivolumab, BMS-936558, and in vivo Toxicology in Non-Human Primates
Wang C, et al. Cancer Immunol Res 2014. doi: 10.1158/2326-6066.CIR-14-0040
“This study characterizes the anti-PD-1 antibody nivolumab in vitro and evaluates its toxicology in cynomolgus monkeys. Flow cytometry was performed using anti-CD3 (UCHT1), anti-CD4 (L200), and anti-CD8 (RPA-T8) antibodies …… Nivolumab binding to PD-1 was confirmed on activated T cells.”

5. Preclinical evaluation of light-activatable, bispecific anti-human CD3 antibody conjugates as anti-ovarian cancer therapeutics
Staroverov RMT, et al. Mol Cancer Ther 2009. doi: 10.1158/1535-7163.MCT-08-1160
“This study evaluates light-activatable UCHT1-folate conjugates in nude mice bearing ovarian cancer xenografts. Flow cytometry confirmed T cell binding using anti-CD3 (UCHT1) antibody …… The conjugate significantly reduced tumor growth compared to non-irradiated controls.”

Frequently Asked Questions (FAQ) about In Vivo Grade Anti-Human CD3 Antibody (Clone: UCHT1)

Question: What are the main applications of Syd Labs’ functional grade UCHT1 antibody?
Answer: Our functional grade ucht1 antibody is explicitly tailored for sensitive preclinical and analytical workflows. It is thoroughly validated for anti-cd3 ucht1 neutralization assays, cellular pathway blockades, and high-throughput bioanalytical configurations. Because of its defined recombinant nature and superior target specificity, it serves as an excellent benchmark for therapeutic antibody development, including pharmacokinetic (PK) modeling and anti-drug antibody (ADA) assay optimization.

Question: Is this recombinant anti-human CD3 UCHT1 antibody supplied with preservatives?
Answer: No, this formulation is entirely carrier-free and preservative-free. To protect fragile primary cells and animal models from chemical toxicity, our low endotoxin anti-human cd3 ucht1 matrix contains absolutely no sodium azide and no BSA or stabilizing protein additives. It is supplied as a 0.2 μM sterile-filtered liquid in pure 1x PBS, maintaining an endotoxin threshold of less than 1 EU/mg via LAL testing.

Question: What is the specific host species and isotype for clone UCHT1?
Answer: This product is engineered as an anti-human cd3 clone ucht1 mouse igg1 (kappa) monoclonal antibody. Every batch features a verified structural purity >95% by SDS-PAGE. Knowing the exact Mouse IgG1 isotype allows investigators to perfectly align their isotype control strategies and calculate non-specific Fc-receptor binding factors during comparative immune pathway tracking.

Question: Can I use this in vivo grade UCHT1 page for routine multi-color flow cytometry?
Answer: While this antibody possesses exceptional binding affinity to the human CD3 epsilon subunit, this specific page (p15855.htm) delivers a high-value, preservative-free format intended for functional biology and ucht1 clone pharmacokinetic ada assays. If you only require routine leukocyte screening or multi-color immunophenotyping, we strongly suggest using our detection-tier UCHT1 format on page p15806.htm, which is pre-stabilized with preservatives for continuous laboratory pipetting.

Question: What are the optimal storage guidelines to preserve the neutralization activity of this antibody?
Answer: To avoid structural degradation and protect its binding capabilities, store short-term (up to 4 weeks) at 2–8°C. For long-term programmatic inventory up to 12 months, we highly recommend dividing the stock into single-use working aliquots and storing at -20°C to -70°C in a manual defrost freezer. Strictly avoid repeating freeze-thaw cycles, which induce protein aggregation and ruin functional neutralization efficacy.