Anti-human CD32 Antibody (Clone: IV.3) | PA007392.h1Fs
$150.00 – $900.00
The human CD32 Fc blocking antibody (IV.3 Fc blocking antibody) is an anti-human Fc receptor antibody for human FcR blocking reagent. Various isotypes are available for certain applications. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007392.h1Fs |
|---|---|
| Name | Anti-human CD32 Antibody (Clone: IV.3) | PA007392.h1Fs |
| Product Name | In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3) |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | cluster of differentiation 32, Fc gamma RIIA and Fc gamma RIIB |
| Clone | IV.3. |
| Isotype | Human IgG1 kappa. |
| Source/Host | The anti-human CD32 monoclonal antibody (clone: IV.3) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant human monoclonal antibody (clone: IV.3) specifically binds to human CD32. |
| Applications | ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD32 protein. Human Fc receptor blocking solution, human Fc receptor blocking reagent, and human Fc receptor blocking antibody. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The In vivo Grade Recombinant Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | The human CD32 Fc blocking antibody (IV.3 Fc blocking antibody) is an anti-human Fc receptor antibody for human FcR blocking reagent. Various isotypes are available for certain applications. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007392.h1Fs: Recombinant Anti-human CD32 Monoclonal Antibody (Clone: IV.3), Human IgG1 Fc Silent Kappa, In vivo Grade
Recombinant human IgG1 monoclonal antibody with Fc silent mutation.
Background
The recombinant IV.3 antibody binds to human CD32 (FcγRII or FCGR2), a cluster of differentiation molecule found on the surface of a variety of immune cells. CD32 is a surface receptor glycoprotein belonging to the Ig gene superfamily, has a low-affinity for the Fc region of IgG antibodies in monomeric form, but high affinity for IgG immune complexes. CD32 has two major functions: cellular response regulation, and the uptake of immune complexes. CD32 consists of Fc receptors FcγRIIa (CD32a), FcγRIIb (CD32b), and FcγRIIc (CD32c).
References of Anti-Human CD32 Monoclonal Antibody (Clone: IV.3)
1. Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.
Lu, et al. Cell Rep. 2024 Apr 23;43(4):113994. doi: 10.1016/j.celrep.2024.113994. PMID: 38555986; PMCID: PMC11031382
“Trogocytosis is an intercellular communication process where immune cells extract surface molecules from interacting cells, but its exploitation by viral pathogens remains incompletely understood. …We demonstrate that HIV-1 exploits autoantibody-enhanced, CD32-driven trogocytosis to facilitate the infection of resting CD4+ T cells, a major viral reservoir. …Blocking the CD32 receptor with the anti-CD32 monoclonal antibody clone IV.3 significantly reduced this receptor transfer and subsequent viral entry, highlighting CD32’s role in HIV pathogenesis.”
2. CD32 Ligation Promotes the Activation of CD4+ T Cells.
Holgado, et al. Front Immunol. 2018 Nov 27;9:2814. doi: 10.3389/fimmu.2018.02814. PMID: 30542352; PMCID: PMC6284025
“The Fc gamma receptor IIa (CD32a) is classically known to be expressed on myeloid cells, but its potential functional role on T cell subsets remains poorly defined. …This study reveals that CD32 is highly expressed on a distinct subset of activated CD4+ T cells and that its ligation provides potent costimulatory signals. …Cross-linking CD32 using the specific monoclonal antibody clone IV.3 enhanced T cell proliferation, activation marker expression, and effector cytokine production.”
3. Inhibition of immunoglobulin E synthesis through FcγRII (CD32) by a mechanism independent of B-cell receptor co-cross-linking.
Qiao, et al. Immunology. 2006 Nov;119(3):418-425. doi: 10.1111/j.1365-2567.2006.02441.x. PMID: 16939665; PMCID: PMC1782155
“The inhibitory receptor FcγRIIb (CD32) is well documented to downregulate B cell responses when co-cross-linked with the B cell antigen receptor (BCR). …We investigated whether CD32 ligation could inhibit IgE synthesis independently of BCR co-ligation, providing an alternative pathway for allergic modulation. …By utilizing the CD32-specific antibody clone IV.3, we demonstrated a profound suppression of IL-4-induced IgE production in human B cells without the need for BCR engagement.”
4. Important roles of CD32 in promoting suppression of IL-4 induced immune responses by a novel anti-IL-4Rα therapeutic antibody.
Wang, et al. Sci Rep. 2019 Jun 28;9(1):9407. doi: 10.1038/s41598-019-45811-9. PMID: 31253816; PMCID: PMC6601543
“Therapeutic antibodies targeting the IL-4 receptor alpha (IL-4Rα) are highly effective in treating type 2 allergic inflammatory diseases, but their optimal function may depend on Fc-gamma receptor interactions. …We show that the suppressive activity of a novel anti-IL-4Rα therapeutic antibody is significantly enhanced in the presence of CD32-expressing immune cells. …The essential role of CD32 engagement was confirmed by blocking the receptor with the anti-CD32 clone IV.3, which diminished the therapeutic efficacy of the antibody in vitro.”
5. Platelet-activating immune complexes identified in critically ill COVID-19 patients suspected of heparin-induced thrombocytopenia.
Brodard, et al. J Thromb Haemost. 2021 May;19(5):1321-1330. doi: 10.1111/jth.15277. PMID: 33544970; PMCID: PMC8014456
“Severe COVID-19 is frequently complicated by coagulopathy and atypical thrombotic events that clinically mimic heparin-induced thrombocytopenia (HIT). …We identified the presence of circulating immune complexes in critically ill COVID-19 patients that strongly activate platelets directly via the low-affinity IgG receptor FcγRIIa (CD32a). …The addition of the CD32-blocking monoclonal antibody clone IV.3 completely abolished the platelet activation induced by patient sera, confirming an Fc-dependent mechanism of thrombosis.”
For more technical references or data sheets regarding the Recombinant Anti-Human CD32 Monoclonal Antibody (Clone: IV.3) formats, or related products from Syd Labs, please contact our scientific support team at message@sydlabs.com.
Syd Labs provides the following in vivo grade recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8)
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3)
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22)
Syd Labs provides the following in vivo grade recombinant anti-mouse CD16, CD32, and CD64 monoclonal antibodies:
Recombinant Anti-mouse CD16/CD32 monoclonal antibody (Clone: 2.4G2)
Syd Labs provides the following recombinant anti-human CD16, CD32, and CD64 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD16 monoclonal antibody (Clone: 3G8) for flow cytometry
Recombinant Anti-human CD32 monoclonal antibody (Clone: IV.3) for flow cytometry
Recombinant Anti-human CD64 monoclonal antibody (Clone: H22) for flow cytometry
Anti-human CD32 (IV.3) from: Anti-human CD32 Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: IV.3): PA007392.h1Fs Syd Labs
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