Anti-mouse CD370 Antibody (Clone: 10B4) | PA007391.r2a

Catalog No.	PA007391.r2a
Product Name	In vivo Grade Recombinant Anti-mouse CD370 Monoclonal Antibody, Rat IgG2a Kappa (Clone: 10B4)
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	CLC9A, Clec9a, DNGR-1, C-type lectin domain family 9 member A
Summary	The in vivo grade recombinant anti-mouse CD370 rat IgG2a monoclonal antibody (Clone: 3H3) was produced in mammalian cells.
Clone	10B4
Isotype	Rat IgG2a Kappa
Specificity/Sensitivity	The in vivo grade recombinant anti-mouse CD370 monoclonal antibody (clone: 10B4) specifically binds to mouse CD370.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD370 protein.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The in vivo grade recombinant anti-mouse CD370 antibodies (clone of 10B4) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007391.r2a: Recombinant Anti-mouse CD370 Monoclonal Antibody(Clone: 10B4), Rat IgG2a Kappa, In vivo Grade

The in vivo grade recombinant anti-mouse CD370 rat IgG2a monoclonal antibody (Clone: 3H3) was produced in mammalian cells.

References of anti-mouse CD370 antibody (Clone: 10B4)

1. The dendritic cell subtype-restricted C-type lectin Clec9A is a target for vaccine enhancement.
Irina Caminschi, et al.Blood. 2008 Oct 15;112(8):3264-73. doi: 10.1182/blood-2008-05-155176. Epub 2008 Jul 30. PMID: 27531783
“Mice were normally injected intravenously with 10 µg of rat anti-Clec9A mAb (10B4) or isotype control mAb1 (IgG2a; eBioscience) or isotype control mAb2 (IgG2a, anti-α-gal; GL117). Serum samples were obtained at 2 to 8 weeks and the level of anti-rat Ig reactivity determined by enzyme-linked immunosorbent assay (ELISA)…The injection of the anti-mClec9A mAb 10B4 alone, without any additional DC activation agents, produced a strong and prolonged anti-rat Ig response (Figure 5).”

For more technical references or data sheets regarding the anti-mouse CD370 antibody (Clone: 10B4) formats, please contact our scientific support team at message@sydlabs.com.

Background of Anti-mouse CD370 Monoclonal Antibody (Clone: 10B4)

The rat anti-mouse CD370 (Clec9A or DNGR-1) monoclonal antibody 10B4 (rat IgG2a kappa) reacts with the mouse CD370 protein. CLEC9A (C-type lectin domain family 9 member A), a transmembrane glycoprotein with a single extracellular C-type lectin domain (CTLD), plays a role in the immune response. As an activation receptor, CLEC9A is expressed on myeloid lineage cells, helps process material from dead cells, recognizes filamentous actin when cell membranes are damaged, and helps present antigens to cytotoxic T cells. CLEC9A has an intracellular cytoplasmic tail with potential signaling motifs. CLEC9A is expressed on a subset of BDCA3+ dendritic cells and CD16- monocytes in humans, and is expressed on CD8+ conventional DCs and plasmacytoid DCs in mice.

Our recombinant 10B4 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse CD370 monoclonal antibody (hybridoma clone name or number: 10B4).

Related Recombinant IgG Reference Antibodies:

In vivo grade recombinant rat IgG2a isotype control antibody

Anti-mouse CD370 Antibody (10B4) from: Recombinant Anti-mouse CD370 Monoclonal Antibody, Rat IgG2a Kappa (Clone: 10B4): PA007391.r2a Syd Labs