Enzyme-linked immunosorbent assay (ELISA) or an enzyme immunoassay (EIA) is used to detect the presence of an antibody or antigen in a sample. Chromogenic, fluorogenic, electrochemiluminescent, and even real-time PCR reporters and substrates are used for various types of ELISA, including indirect ELISA, sandwich ELISA, competitive ELISA, and reverse ELISA.

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