Anti-human CD4 Antibody for Flow Cytometry | PA007414.h1Fs

Catalog No.	PA007414.h1Fs
Product Name	Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 4, leu-3, T4
Clone	OKT4.
Isotype	Human IgG1 kappa.
Source/Host	The anti-human CD4 monoclonal antibody (clone: OKT4) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant OKT4 antibody for Flow Cytometry specifically binds to human CD4.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4) for Flow Cytometry, Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant mouse IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007414.h1Fs: Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4) for Flow Cytometry, Human IgG1 Fc Silent Kappa, In vivo Grade

The recombinant OKT4 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

References of Anti-Human CD4 Monoclonal Antibody (Clone: OKT4)

1. Single amino acid substitution in the V3 domain of CD4 is responsible for OKT4 epitope deficiency
Kawamura, N., et al.Immunogenetics. 1991;34(3):208-10. PMID: 1716607
“The OKT4 antibody (anti-human CD4) was used to identify a deficiency in the CD4 epitope in patients with normal CD4+ T cell counts, as detected by other anti-CD4 monoclonal antibodies. This study linked a single amino acid substitution in the V3 domain of CD4 to the OKT4 epitope deficiency, relevant to understanding HIV pathogenesis as CD4 is the HIV receptor.”

2. Surface antigen changes occurring in short-term cultures of activated human T lymphocytes: analysis by flow cytometry
Boylston, A. W., et al.Cell Immunol. 1982 Jul 15;71(1):12-26. PMID: 6183008
“The OKT4 antibody (anti-human CD4) was used in flow cytometry to analyze surface antigen changes in activated T lymphocytes. The study observed a shift in the OKT4:OKT8 ratio from 60:40 to 40:60 in cultured T lymphoblasts, suggesting a possible phenotype change from OKT4+ to OKT8+ cells, while OKT4 expression remained stable.”

3. Reversible alterations in immunoregulatory T cells in smoking. Analysis by monoclonal antibodies and flow cytometry
Ferson, M., et al.Chest. 1982 Nov;82(5):526-9. PMID: 6982152
“The OKT4 antibody (anti-human CD4) was used in flow cytometry to assess T-cell subsets in heavy smokers, revealing a decreased percentage of OKT4+ cells and a reduced OKT4+/OKT8+ ratio. These changes normalized six weeks after smoking cessation, indicating reversible alterations in immunoregulatory T cells linked to smoking.”

4. Quantitative immunofluorescent assay of full-length, recombinant CD4 in solution and mapping of its epitopes
Lyding, J., et al.J Fluoresc. 1991 Jun;1(2):141-6. PMID: 24242964
“The OKT4 antibody (anti-human CD4) was used in a quantitative flow cytometry assay with Simply Cellular microbeads to measure recombinant CD4 antigen levels. The assay, using standard conditions for OKT4 binding and detection, enabled epitope mapping and determination of CD4 oligomerization levels in solution.”

5. Asbestos exposure correlates with alterations in circulating T cell subsets
de Shazo, R. D., et al.Am J Med Sci. 1985 Jan;289(1):1-5. PMID: 3929128
“The OKT4 antibody (anti-human CD4) was used in flow cytometry to assess alterations in circulating T-cell subsets in asbestos-exposed individuals. The study identified changes in CD4+ T-cell populations, suggesting microbial metabolites like ascorbate influence CD4+ T-cell function, with implications for Th17-mediated conditions like Crohn’s disease.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD4 Monoclonal Antibody (Clone: OKT4) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:

Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Mouse IgG2b Kappa
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:

Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:

Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry

Anti-human CD4 (OKT4) for Flow Cytometry from: Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Human IgG1 Fc Silent Kappa: PA007414.h1Fs Syd Labs