Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A), Mouse IgG2a Kappa PA007413.m2a Syd Labs

Anti-human CD4 Antibody for Flow Cytometry | PA007413.m2a

$100.00 – $200.00

Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007413.m2a
Product Name	Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A), Mouse IgG2a Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 4, leu-3, T4
Clone	OKT4A
Isotype	Mouse IgG2a kappa.
Source/Host	The anti-human CD4 monoclonal antibody (clone: OKT4A) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant OKT4A antibody for Flow Cytometry specifically binds to human and non-human primate CD4.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A) for Flow Cytometry, Mouse IgG2a Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007413.m2a: Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4A) for Flow Cytometry, Mouse IgG2a Kappa, In vivo Grade

The recombinant OKT4A antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

References of Anti-Human CD4 Monoclonal Antibody (Clone: OKT4A)

1. The β-Chemokine Receptors CCR3 and CCR5 Facilitate Infection by Macrophage-Tropic Strain HIV-1
Doms, R. W., et al.Cell. 1998 Jun 12;93(6):1009-15. PMID: 10329570
“The OKT4A antibody (anti-human CD4, PE-conjugated) was used in flow cytometry to assess CD4 expression in cells with low CD4 levels, reducing non-specific binding and enhancing gp120 binding to CCR5. OKT4A staining confirmed CD4 dependency and inhibited CCR5-mediated fusion in a dose-dependent manner, as analyzed by flow cytometry.”

2. Restoration of Cell Surface CD4 Expression in Simian Immunodeficiency Virus-Infected Cells by Intracellular Expression of a Soluble CD4 Molecule
Turner, J. D., et al.J Virol. 1992 Nov;66(11):6802-5. PMID: 1380959
“The OKT4A antibody (anti-human CD4) was used in flow cytometry to measure CD4 surface expression in SIV-infected cells. OKT4A staining showed restored wild-type CD4 levels after soluble CD4 transfection, with controls lacking OKT4A showing no fluorescence shift, confirming prevention of CD4 down-regulation.”

3. Mapping the Paratope of Anti-CD4 Recombinant Fab 13B8.2 by Alanine Scanning Mutagenesis
Thali, M., et al.J Biol Chem. 1993 Sep 25;268(27):20785-90. PMID: 8391275
“The OKT4A antibody (anti-human CD4) was used in flow cytometry to assess binding to mutant CD4 on transfected cells. OKT4A reactivity was lost at alanine mutants (residues 58 and 62), confirming paratope overlap with 13B8.2. OKT4A served as a positive control for high-affinity CD4 binding, measured by mean fluorescence intensity.”

4. Internalization and Degradation of an Anti-CD4 Monoclonal Antibody in Human T Lymphoblasts
König, R., et al.Mol Immunol. 1993 Feb;30(3):231-9. PMID: 8468487
“The OKT4A antibody (anti-human CD4) was used as the primary probe in flow cytometry to track CD4 internalization in T lymphoblasts. Surface-bound OKT4A decreased within 30 minutes, with lysosomal inhibitors blocking degradation, confirming rapid internalization and degradation of the OKT4A-CD4 complex.”

5. Human Immunodeficiency Virus Glycoprotein (gp120) Induction of Monocyte Arachidonic Acid Metabolism
Klein, E., et al.Proc Natl Acad Sci U S A. 1989 Jan;86(2):621-5. PMID: 2536171
“The OKT4A antibody (anti-human CD4) was used in flow cytometry to confirm CD4 expression on monocytes, with 80% OKT4A positivity. OKT4A-positive cells showed increased arachidonic acid release upon gp120 stimulation, with the OKT4A epitope required for gp120 signaling, while controls lacking OKT4A showed no response.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD4 Monoclonal Antibody (Clone: OKT4A) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.