Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Mouse IgG2b Kappa PA007384.m2b Ushelf

Anti-human CD4 Antibody, Clone OKT4 | PA007384.m2b

Name: Anti-human CD4 Antibody, Clone OKT4 | PA007384.m2b
Brand: Syd Labs
SKU: PA007384.m2b
Availability: InStock

Catalog No.: PA007384.m2b | Isotype: Mouse IgG2b kappa | In Vivo Grade

$150.00 – $900.00

Recombinant mouse IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007384.m2b
Product Name	In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Mouse IgG2b Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 4, leu-3, T4
Clone	OKT4.
Isotype	Mouse IgG2b kappa.
Source/Host	The anti-human CD4 monoclonal antibody (clone: OKT4) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: OKT4) specifically binds to human CD4.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD4 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Mouse IgG2b Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007384.m2b: Recombinant Anti-human CD4 Monoclonal Antibody(Clone: OKT4), Mouse IgG2b Kappa, In vivo Grade

Background of Anti-human CD4 Monoclonal Antibody(Clone: OKT4)

The recombinant OKT4 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

References of Anti-Human CD4 Monoclonal Antibody (Clone: OKT4)

1. Administration of prednisolone in vivo affects the ratio of OKT4/OKT8 and the LDH-isoenzyme pattern of human T lymphocytes
Tenbergen, F., et al. Clin Immunol Immunopathol. 1984 Feb;30(2):191-202. doi: 10.1016/0090-1229(84)90010-2. PMID: 6607798
“The OKT4 antibody (anti-human CD4) was used to assess T lymphocyte subsets in healthy volunteers treated with prednisolone (10, 30, or 60 mg). Prednisolone induced T lymphocytopenia, preferentially affecting OKT4+ cells, leading to a decreased OKT4/OKT8 ratio and altered lactate dehydrogenase isoenzyme patterns, with effects varying by dose and time post-administration.”

2. In vivo activated cytotoxic T cells in the thyroid infiltrate of patients with Hashimoto’s thyroiditis
Del Prete, G. F., et al. Clin Exp Immunol. 1986 Jul;65(1):140-7. PMID: 3024884
“The OKT4 antibody (anti-human CD4) was used with complement for negative selection to enrich T8+ cells in thyroid-infiltrating lymphocytes from Hashimoto’s thyroiditis patients. High T8+ cell proportions with an inverted T4/T8 ratio were observed, and OKT4 helped confirm low CD4+ cell presence in TAC+ T cell populations.”

3. Human CD4 modulation in vivo induced by antibody treatment
Horneff, G., et al. Clin Immunol Immunopathol. 1993 Jan;66(1):80-90. doi: 10.1006/clin.1993.1011. PMID: 8440076
“The OKT4 antibody (anti-human CD4) was used to monitor CD4 modulation in vivo after anti-CD4 antibody (MAX.16H5) treatment. CD4 antigen density was reduced to 30% within 1 hour and remained down-modulated for up to 28 days, with depletion of CD4+ cells observed in both responders and non-responders to therapy.”

4. Dramatic rise in plasma viremia after CD8(+) T cell depletion in simian immunodeficiency virus-infected macaques
Jin, X., et al. J Exp Med. 1999 Mar 15;189(6):991-1000. doi: 10.1084/jem.189.6.991. PMID: 10075982
“The OKT8F antibody (anti-human CD8) was used to deplete CD8+ cells in SIV-infected macaques, resulting in a 99.9% reduction in peripheral blood CD8 cells and a 70-fold increase in plasma viremia. The OKT4 antibody (anti-human CD4) was used to assess the resting CD4+ T cell pool, revealing increased productively infected cells.”

5. Human in vivo antigenic modulation induced by the anti-T cell OKT3 monoclonal antibody
Rijlaarsdam, J. U., et al. Eur J Immunol. 1982 Nov;12(11):979-82. doi: 10.1002/eji.1830121116. PMID: 6759145
“The OKT4 antibody (anti-human CD4) was used to confirm stable CD4 expression on T cells during OKT3 treatment, which rapidly modulated the OKT3 epitope within 30 minutes via internalization. OKT4 and OKT8 epitopes remained unaffected, with OKT4 aiding in monitoring T-cell populations during transient lymphocytopenia.”