Anti-human CD4 Antibody for Flow Cytometry | PA007415.m1

Catalog No.	PA007415.m1
Product Name	Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 4, leu-3, T4
Clone	13B8.2.
Isotype	Mouse IgG1 kappa.
Source/Host	The anti-human CD4 monoclonal antibody (clone: 13B8.2) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant 13B8.2 antibody for Flow Cytometry specifically binds to human CD4.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2) for Flow Cytometry, Mouse IgG1 Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007415.m1: Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2) for Flow Cytometry, Mouse IgG1 Kappa, In vivo Grade

The recombinant 13B8.2 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

References of Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2)

1. In vitro antitumoral activity of baculovirus-expressed chimeric recombinant anti-CD4 antibody 13B8.2 on T-cell lymphomas
Troadec, S., et al.J Immunother. 2007 Feb-Mar;30(2):190-202. PMID: 17273799
“The chimeric recombinant anti-CD4 antibody 13B8.2, expressed in baculovirus, was tested for antitumoral activity against T-cell lymphomas using flow cytometry. The 13B8.2 antibody induced apoptosis in CD4+ Jurkat cells, as confirmed by Annexin V staining, and significantly reduced CD4+ T cells in treated lymphoma cell lines. Cell viability was assessed after 48 hours using MTT assays, and the efficacy of 13B8.2 was compared with other anti-CD4 antibodies in vitro.”

2. An anti-CD4 (CDR3-loop) monoclonal antibody inhibits human immunodeficiency virus type 1 envelope glycoprotein-induced apoptosis
Guillerm, C., et al.Virology. 1998 Sep 15;248(2):254-63. PMID: 9743188
“The anti-CD4 monoclonal antibody 13B8.2, targeting the CDR3-like region of CD4, was used to assess HIV-1 envelope glycoprotein-induced apoptosis via flow cytometry. Preincubation with 13B8.2 significantly reduced apoptosis in HIV-1-infected T cells and blocked CD4 crosslinking by gp120. The study also demonstrated that 13B8.2 inhibits syncytium formation in vitro, with cell surface CD4 expression quantified using fluorescently labeled antibodies.”

3. The Cytoplasmic Tail of CD4 Is Required for Inhibition of HIV-1 Env-Mediated Cell Fusion
Albright, C. A., et al.J Virol. 1997 Jun;71(6):4686-91. PMID: 9171443
“The 13B8.2 antibody (anti-human CD4 antibody) was used to detect CD4 expression on transfected HeLa cells by flow cytometry to study HIV-1 envelope-mediated cell fusion. Mutations in the CD4 cytoplasmic tail abolished the inhibitory effect on cell fusion, while flow cytometry confirmed that CD4 surface expression remained unchanged. HIV-1 envelope glycoprotein binding was assessed through co-culture experiments.”

4. CD4 is required for inhibition of human immunodeficiency virus type 1 replication by antibodies that bind to the immunoglobulin CDR3-like region in domain 1 of CD4
Benkirane, M., et al.J Virol. 1995 Nov;69(11):6904-10. PMID: 7568058
“The anti-CD4 antibody 13B8.2, targeting the immunoglobulin CDR3-like region, was used to inhibit HIV-1 replication in Jurkat cells, with CD4 expression monitored by flow cytometry. The 13B8.2 antibody effectively blocked HIV-1 provirus transcription without significantly altering CD4 surface levels. Inhibition of HIV-1 entry was confirmed using syncytium formation assays.”

5. An antibody that binds the immunoglobulin CDR3-like region of the CD4 molecule inhibits provirus transcription in HIV-infected T cells
Benkirane, M., et al.EMBO J. 1993 Dec;12(12):4909-21. PMID: 8102412
“The 13B8.2 antibody (anti-human CD4 mAb), targeting the immunoglobulin CDR3-like region of CD4, was used to inhibit provirus transcription in HIV-1-infected Jurkat cells, with CD4 expression quantified by flow cytometry. The study showed that 13B8.2 does not affect HIV-1 entry but targets post-entry events, with stable CD4 expression confirmed during treatment.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:

Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Mouse IgG1 Kappa
Non-conjugated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:

Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:

Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry

Anti-human CD4 (13B8.2) for Flow Cytometry from: Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa: PA007415.m1 Syd Labs