Anti-Mouse CD8a Antibody(YTS 169.4, Rat IgG2b) | PA007166.r2b

Catalog No.	PA007166.r2b
Product Name	In Vivo Grade Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone YTS 169.4), Rat IgG2b Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	CD8 alpha, T-cell surface glycoprotein CD8 alpha chain, CD_antigen CD8a
Summary	The in vivo grade recombinant anti-mouse CD8a monoclonal antibody, rat IgG2b was produced in mammalian cells.
Clone	YTS 169.4.
Isotype	rat IgG2b, kappa.
Specificity/Sensitivity	CD8a
Applications	Western blot, immunohistochemistry (IHC), Flow Cytometry (FC), and in vivo CD8+ T cell depletion.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Anti-Mouse CD8a Monoclonal Antibody (Clone YTS 169.4) are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Note	Recombinant anti-mouse CD8a antibodies from the variable region sequences of the rat anti-mouse CD8a antibody (clone number: YTS 169.4) are produced from mammalian cells and good for in vitro and in vivo studies.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007166.r2b: Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone YTS 169.4), Rat IgG2b Kappa, In Vivo Grade

The rat anti-mouse CD8a monoclonal antibody YTS 169.4 (rat IgG2b kappa) specifically recognizes the mouse CD8a protein (T-cell surface glycoprotein CD8 alpha chain), encoded by the CD8A gene. This protein forms part of the dimeric CD8 complex and plays a crucial role in cell-mediated immunity and T-cell development. CD8A has been extensively studied as a potential biomarker for various diseases, including inflammatory disorders and cancers.

Our recombinant YTS 169.4 antibodies contain either partial (variable regions) or complete amino acid sequences of the original hybridoma-derived antibody (clone YTS 169.4). These antibodies demonstrate potent in vivo CD8+ T cell depletion activity through Fc-mediated mechanisms.

Syd Labs offers two high-performance variants of the Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone YTS 169.4) for advanced immunology research: one with Mouse IgG2a Kappa and the other with Rat IgG2b Kappa isotypes, each tailored to meet specific experimental needs. Both antibodies are produced using cutting-edge recombinant technology, ensuring exceptional batch-to-batch consistency and purity, making them ideal for in vivo applications such as T cell depletion studies. They specifically target the mouse CD8a antigen, a key marker for cytotoxic T cells, and are essential for investigating cell-mediated immunity and immune system dynamics. The Mouse IgG2a Kappa variant is particularly suited for experiments requiring effector functions like antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC), while the Rat IgG2b Kappa variant excels in minimizing background in mouse models by reducing cross-reactivity. Both antibodies are versatile for use in flow cytometry, immunohistochemistry (IHC), and T cell functional assays, providing researchers with reliable tools to advance their studies in T cell biology and beyond. Choose Syd Labs for precision and excellence in your scientific discoveries.

References for Anti-Mouse CD8a Monoclonal Antibody (Clone: YTS 169.4)

1. Influenza-Activated ILC1s Contribute to Antiviral Immunity Partially Influenced by Differential GITR Expression.
Vashist, N., et al.Front Immunol. 2018 Mar 22;9:505. PMID: 29623077
“To evaluate the role of cytotoxic T lymphocytes in viral clearance, monoclonal anti-mouse CD8 antibody (clone YTS 169.4) was administered intraperitoneally (200 µg/animal in 200 µl PBS) at days 0 and 3 post-infection in an H1N1 influenza model. The protocol demonstrated high efficacy in depleting systemic CD8+ T cells to dissect cell-mediated immune defense mechanisms.”

2. Myeloid Cell Arg1 Inhibits Control of Arthritogenic Alphavirus Infection by Suppressing Antiviral T Cells.
Burrack, K. S., et al.PLoS Pathog. 2015 Oct 5;11(10):e1005191. PMID: 26436766
“This study utilized the rat IgG2b anti-mouse CD8 antibody (clone YTS 169.4) for in vivo depletion studies in WT and mutant mice on days 7 and 12 post-infection. Splenic staining for CD3 and CD8α confirmed that the YTS 169.4 clone successfully depleted approximately 98% of target CD8+ T cell subsets, verifying its potency in inflammatory disease models.”

3. TGF-β signalling is required for CD4+ T cell homeostasis but dispensable for regulatory T cell function.
Sledzinska, A., et al.PLoS Biol. 2013 Oct;11(10):e1001674. PMID: 24115907
“For targeted elimination of CD8+ T cells post-bone marrow transplantation, mice were injected at days 15 and 17 with 250 µg of anti-CD8 antibody (clone YTS 169.4), followed by weekly maintenance doses of 100 µg. Flow cytometric analysis confirmed robust depletion efficiency, facilitating the investigation of lymphoproliferative diseases and leukemia progression.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD8a Monoclonal Antibody (Clone YTS 169.4) formats, please contact our scientific support team at message@sydlabs.com.

We provide the following recombinant anti-human CD8a monoclonal antibodies:
Recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry

We provide the following recombinant anti-mouse CD8a monoclonal antibodies:
Recombinant anti-mouse CD8a antibodies (clone 2.43), In vivo grade
Recombinant anti-mouse CD8a antibodies (clone YTS 169.4), In vivo grade
Recombinant anti-mouse CD8a antibodies (clone YTS 105.18), In vivo grade

Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
Recombinant anti-mouse CD8b antibodies (clone YTS 156.7), In vivo grade

Anti-Mouse CD8a Antibody(YTS 169.4) from: In Vivo Grade Recombinant Anti-Mouse CD8a Rat IgG2b Kappa Monoclonal Antibody (Clone YTS 169.4): PA007166.r2b Syd Labs