Description

PA007418.m2a: Non-conjugated Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8) for Flow Cytometry, Mouse IgG2a Kappa, In vivo Grade

The recombinant OKT8 antibody binds to the human CD8 protein (cluster of differentiation 8), a type I transmembrane glycoprotein and the co-receptor for the T-cell receptor (TCR). Along with the TCR, the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell-antigen interactions. The CD8 protein is predominantly expressed on the surface of cytotoxic T cells, but can also be found on natural killer cells, cortical thymocytes, and dendritic cells. The CD8 molecule is a marker for cytotoxic T cell population.

References of Anti-Human CD8a Monoclonal Antibody (Clone: OKT8)

1. T-cell receptor gene transfer exclusively to human CD8+ cells in a mouse model
Sadelain, M., et al.Blood. 2012 Nov 29;120(22):4334-41. PMID: 23068516
“The OKT8 antibody (anti-human CD8a) was used in flow cytometry to confirm specific transduction of CD8+ T cells in a mouse model. OKT8 staining showed high transduction efficiency in the CD8+ population compared to CD4+ cells, with OKT8-positive cells gated for sorting, enhancing tumor killing in transduced cells.”

2. Phenotypic and functional characterization of human cytotoxic T cells lacking the OKT8 antigen
Landay, A., et al.J Immunol. 1983 Aug;131(2):1016-9. PMID: 6603416
“The OKT8 antibody (anti-human CD8a) was used in flow cytometry to identify cytotoxic T cells lacking the OKT8 antigen. OKT8-negative CTL clones exhibited normal cytolytic activity, indicating that the absence of OKT8 did not impair function, with OKT8 serving as a marker for conventional CTL.”

3. A human NK and K cell subset shares with cytotoxic T cells expression of the antigen recognized by antibody OKT8
Perussia, B., et al.J Immunol. 1983 Jul;131(1):223-31. PMID: 6408172
“The OKT8 antibody (anti-human CD8a) identified a subset of NK and K cells expressing the OKT8 antigen, detected in 20-30% of NK cells via flow cytometry. OKT8-positive NK cells showed cytotoxic activity similar to T cells, and OKT8 was used to isolate this subset for further analysis.”

4. Flow cytometric studies of the binding of monoclonal antibodies (OKT3, OKT4 and OKT8) to human T cells
Boyer, K. M., et al.J Immunol Methods. 1988 Dec 9;115(1):55-62. PMID: 3058805
“The OKT8 antibody (anti-human CD8a) was analyzed for binding to CD8+ T cells via flow cytometry at various temperatures. OKT8 showed endothermic binding characteristics, with increased fluorescence intensity at higher temperatures, and its affinity was calculated from flow cytometry data for cytometric detection.”

5. Interactions of lectins and monoclonal antibodies with human T-cell subsets defined by OKT4 and OKT8 antibodies
Reinherz, E. L., et al.J Immunol. 1983 Jan;130(1):67-72. PMID: 6601134
“The OKT8 antibody (anti-human CD8a) was used in flow cytometry to define cytotoxic T cells, with lectin treatment enhancing OKT8 staining. OKT8-defined subsets showed differential lectin responses, and binding interactions were quantified by mean fluorescence intensity in flow cytometry assays.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD8a Monoclonal Antibody (Clone: OKT8) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies for flow cytometry:

Non-conjugated Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Mouse IgG2a Kappa
Non-conjugated Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Human IgG1 Fc Silent Kappa

Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:

In vivo grade recombinant anti-human CD8a antibodies (clone OKT8)
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry

Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:

In vivo grade recombinant anti-mouse CD8a antibodies (clone 2.43)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 169.4)
In vivo grade recombinant anti-mouse CD8a antibodies (clone YTS 105.18)

Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:

In vivo grade recombinant anti-mouse CD8b antibodies (clone YTS 156.7)

Anti-human CD8a (OKT8) for Flow Cytometry from: Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Mouse IgG2a Kappa: PA007418.m2a Syd Labs