Anti-human CD8a Antibody, clone okt8 | PA007388.m2a
$150.00 – $900.00
Recombinant mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007388.m2a |
|---|---|
| Product Name | In vivo Grade Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Mouse IgG2a Kappa |
| Data Sheet | 📄 Download Product Data Sheet (PDF) |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | cluster of differentiation 8, leu-2, T8 |
| Clone | OKT8. |
| Isotype | Mouse IgG2a kappa. |
| Source/Host | The anti-human CD8a monoclonal antibody (clone: OKT8) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant mouse monoclonal antibody (clone: OKT8) specifically binds to human CD8a. |
| Applications | ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD8 protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The In vivo Grade Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Mouse IgG2a Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007388.m2a: Recombinant Anti-human CD8a Monoclonal Antibody (Clone: OKT8), Mouse IgG2a Kappa, In vivo Grade
Background of Anti-human CD8a Monoclonal Antibody (Clone: OKT8)
The recombinant OKT8 antibody binds to the human CD8 protein (cluster of differentiation 8), a type I transmembrane glycoprotein and the co-receptor for the T-cell receptor (TCR). Along with the TCR, the CD8 co-receptor plays a role in T cell signaling and aiding with cytotoxic T cell-antigen interactions. The CD8 protein is predominantly expressed on the surface of cytotoxic T cells, but can also be found on natural killer cells, cortical thymocytes, and dendritic cells. The CD8 molecule is a marker for cytotoxic T cell population.
The anti-human CD8a antibody is a monoclonal antibody that specifically targets the CD8 alpha (CD8a) chain, a transmembrane glycoprotein expressed on the surface of cytotoxic T cells and a subset of natural killer (NK) cells. CD8a forms either heterodimers with CD8 beta (αβ) or homodimers (αα), functioning as a co-receptor for the T cell receptor (TCR) by binding to MHC Class I molecules. This interaction enhances T cell activation and supports immune responses against virus-infected cells and tumor cells.
Anti-human CD8a antibodies selectively bind to the 32-34 kDa CD8 alpha chain, making them essential for identifying CD8-positive cytotoxic T lymphocytes (CTLs) and certain NK cell subsets. Popular clones include RPA-T8 and SK1, both mouse IgG1 monoclonal antibodies widely recognized for their specificity and reliability in detecting human CD8a.
In immunology and diagnostics, anti-human CD8a antibodies are critical tools for:
- Flow Cytometry: Quantifying and phenotyping CD8+ T cells in blood or tissue samples, often used with fluorochrome conjugates (e.g., FITC, PE, APC).
- Immunohistochemistry (IHC): Visualizing CD8+ cells in tissue sections to assess immune infiltration in infections, cancer, or autoimmune conditions.
- Cell sorting, immune profiling, and studying adaptive immunity.
CD8a-expressing cells, primarily cytotoxic T cells, play a vital role in eliminating infected or malignant cells. Anti-human CD8a antibodies help researchers and clinicians monitor these populations, contributing to insights in cancer immunology, infectious diseases, and transplantation.
References of Anti-Human CD8a Monoclonal Antibody (Clone: OKT8)
1. Steroid-sensitive mechanism of soluble immune response suppressor production in steroid-responsive nephrotic syndrome
Tomana, M., et al.J Clin Invest. 1987 Jan;79(1):257-64. doi: 10.1172/JCI112792. PMID: 3793925
“The OKT8 antibody (anti-human CD8a) was used to identify and deplete OKT8+ suppressor T cells in serum from patients with steroid-responsive nephrotic syndrome. OKT8 depletion eliminated soluble immune response suppressor (SIRS) activity, confirming its production by OKT8+ cells, which was inhibited by steroids both in vitro and in vivo.”
2. Circulating activated suppressor T lymphocytes in aplastic anemia
Gascon, P., et al.N Engl J Med. 1985 Jan 31;312(5):257-65. doi: 10.1056/NEJM198501313120501. PMID: 2981406
“The OKT8 antibody (anti-human CD8a) identified activated suppressor T lymphocytes in aplastic anemia patients via flow cytometry. OKT8 depletion restored hematopoiesis in vitro, and in vivo OKT8+ cell counts correlated with disease severity, with targeted treatment improving outcomes in some patients.”
3. Regulation of normal human blood neutrophilic, macrophagic, and eosinophilic committed stem cell proliferation by autologous blood T lymphocyte subsets
Bagby, G. C. Jr., et al.Blood. 1984 Feb;63(2):356-63. PMID: 6229296
“The OKT8 antibody (anti-human CD8a) identified OKT8+ T lymphocytes that inhibited committed stem cell proliferation in a contact-independent manner. OKT8 depletion enhanced stem cell growth in culture, suggesting a regulatory role for OKT8+ cells in myelopoiesis in vivo.”
4. Control of polyclonal immunoglobulin production from human lymphocytes by leukotrienes; leukotriene B4 induces an OKT8 (anti-human CD8a) (+), radiosensitive suppressor cell from resting, human OKT8(-) T cells
Rola-Pleszczynski, M., et al.J Clin Invest. 1984 May;73(5):1334-40. doi: 10.1172/JCI111338. PMID: 6090503
“The OKT8 antibody (anti-human CD8a) identified radiosensitive OKT8+ suppressor cells induced by leukotriene B4 from OKT8- T cells. These cells suppressed B cell differentiation and immunoglobulin production in vivo, with OKT8 used to confirm the suppressor cell phenotype in flow cytometry assays.”
5. Requirement for OKT8+ suppressor cell proliferation for suppression by human newborn T cells
Hayward, A. R., et al.Clin Exp Immunol. 1981 Sep;45(3):468-74. PMID: 6461445
“The OKT8 antibody (anti-human CD8a) identified increased OKT8+ suppressor cells in PWM-stimulated newborn lymphocyte cultures. OKT8 depletion with complement or 50 microM deoxyguanosine reduced suppressor activity, indicating that OKT8+ cell proliferation is required for suppression, with implications for neonatal tolerance in vivo.”
For more technical references or data sheets regarding the Recombinant Anti-Human CD8a Monoclonal Antibody (Clone: OKT8) formats, please contact our scientific support team at message@sydlabs.com.
Syd Labs provides the following recombinant anti-human CD8a monoclonal antibodies:
Recombinant anti-human CD8a antibodies (clone OKT8)，In vivo grade
Recombinant anti-human CD8a antibodies (clone OKT8) for flow cytometry
Syd Labs provides the following recombinant anti-mouse CD8a monoclonal antibodies:
Recombinant anti-mouse CD8a antibodies (clone 2.43), In vivo grade
Recombinant anti-mouse CD8a antibodies (clone YTS 169.4), In vivo grade
Recombinant anti-mouse CD8a antibodies (clone YTS 105.18), In vivo grade
Syd Labs provides the following recombinant anti-mouse CD8b monoclonal antibodies:
Recombinant anti-mouse CD8b antibodies (clone YTS 156.7), In vivo grade
Anti-human CD8a (OKT8) from: Anti-human CD8a Monoclonal Antibody (Clone: OKT8): PA007388.m2a Syd Labs
