Anti-mouse CD16CD32 Monoclonal Antibody (Clone 2.4G2) PA007389.m2a Ushelf

Anti-mouse CD16/CD32 Antibody (Clone: 2.4G2) | PA007389.m2a

$150.00 – $900.00

The mouse CD16/CD32 Fc blocking antibody (2.4G2 Fc blocking antibody) is an anti-mouse Fc receptor antibody for mouse FcR blocking reagent. Various isotypes are available for certain applications. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007389.m2a
Product Name	In vivo Grade Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody, Mouse IgG2a Kappa (Clone: 2.4G2)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Mouse Fc Receptor Blocking Antibody, Mouse 2.4G2 Fc Block, Mouse CD16/CD32 Fc Block
Summary	The anti-mouse CD16/CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.
Clone	2.4G2
Isotype	Mouse IgG2a Kappa
Specificity/Sensitivity	The in vivo grade recombinant rat monoclonal antibody (clone: 2.4G2) specifically binds to mouse CD16 and CD32.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse CD16 and CD32 protein. Mouse Fc receptor blocking solution, mouse Fc receptor blocking reagent, and mouse Fc receptor blocking antibody.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The in vivo grade recombinant anti-mouse CD16/CD32 monoclonal antibody, Mouse IgG2a Kappa (Clone: 2.4G2) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	The mouse CD16/CD32 Fc blocking antibody (2.4G2 Fc blocking antibody) is an anti-mouse Fc receptor antibody for mouse FcR blocking reagent. Various isotypes are available for certain applications. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007389.m2a: Recombinant Anti-mouse CD16/CD32 Monoclonal Antibody(Clone: 2.4G2), Mouse IgG2a Kappa, In vivo Grade

The anti-mouse CD16/CD32 monoclonal antibody (clone: 2.4G2) was produced in mammalian cells.

The most popular mouse Fc blocking reagents include:
Anti-Mouse CD16/CD32 (Clone 2.4G2): For flow cytometry, immunohistochemistry (IHC), and immunoprecipitation (IP). It is highly specific for Fc?RII (CD32) and Fc?RIII (CD16), and reduces background staining; it may not block all Fc receptors. Clone X54-5/7.1 is the most widely used anti-mouse Fc?RI (CD64) clone for Fc blocking in flow cytometry and functional assays. It prevents non-specific Fc receptor binding on monocytes, macrophages, and dendritic cells, improving antibody specificity.
Normal mouse serum, purified mouse IgGs from mouse serum, or mixes of mouse isotype controls: For general Fc blocking in IHC, and immunofluorescence (IF). It is cheap and easy to use but it is less specific, and may even introduce unwanted immunoglobulins. Our recombinant mouse IgG antibody mixes are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
Purified rat IgG or isotype controls: For flow cytometry. It can serve as a control to block Fc receptors but it is non-specific for Fc receptors. Syd Labs supply various rat IgG isotype controls.
Bovine Serum Albumin (BSA) or FBS: For general blocking in Western Blot (WB) and IHC. They are readily available to reduce non-specific binding but less effective at Fc receptor blocking.
Commercial Fc blocking solutions: For flow cytometry, IHC, and functional assays. They are normally pre-optimized and highly effective but more expensive than DIY solutions.

Best choice of mouse Fc blocking reagents based on your application are:
For flow cytometry: Anti-mouse CD16/CD32 (Clone 2.4G2).
For Immunohistochemistry (IHC) and Immunofluorescence (IF): Normal mouse serum, purified mouse or rat IgGs or isotype controls or commercial Fc blocking kits. Our recombinant mouse IgG antibody mixes and recombinant rat IgG isotype controls are affordable; the ratio of various IgGs is optimized and adjustable without any unwanted immunoglobulins.
For Western Blot and ELISA: BSA or FBS.
For functional assays (e.g., blocking Fc-mediated effects): Commercial Fc blocking reagents.

References of Anti-Mouse CD16/CD32 Monoclonal Antibody (Clone: 2.4G2)

1. Signaling through the inhibitory Fc receptor Fc?RIIB induces CD8 T cell apoptosis to limit T cell immunity.
Yao, et al.eLife. 2020 Jun 30;9:e54540. doi: 10.7554/eLife.54540. PMID: 32602844; PMCID: PMC7326381
“We found that signaling through the inhibitory Fc receptor Fc?RIIB on T cells promotes apoptosis. … This pathway serves as a critical checkpoint to limit CD8+ T cell-mediated immunity. … Targeting this pathway with blocking antibodies like 2.4G2 can rescue T cells from apoptosis and enhance anti-tumor responses.”

2. Accelerated clearance and degradation of cell free HIV by neutralizing antibodies occurs via Fc?RIIb on liver sinusoidal endothelial cells by endocytosis.
Ganesan, et al.PLoS Pathog. 2021 Mar 11;17(3):e1009367. doi: 10.1371/journal.ppat.1009367. PMID: 33690651; PMCID: PMC7946731
“Neutralizing antibodies (nAbs) are key to preventing HIV infection, but their role in clearing cell-free virus from circulation is less clear. … Here, we show that cell-free HIV-1 opsonized with nAbs is rapidly cleared from the circulation via liver sinusoidal endothelial cells (LSECs). … This clearance and subsequent endocytosis are mediated by the inhibitory Fc receptor Fc?RIIb, which can be blocked by anti-Fc?RIIb antibodies.”

3. Plasmacytoid Dendritic Cells Take Up Opsonized Antigen Leading to CD4+ and CD8+ T Cell Activation In Vivo.
Bánki, et al.J Immunol. 2010 Jun 15;184(12):6607-15. doi: 10.4049/jimmunol.0903335. PMID: 20483731; PMCID: PMC2884144
“Plasmacytoid dendritic cells (pDCs) are well known for their capacity to produce massive amounts of type I IFN. … In this study, we demonstrate that pDCs can efficiently take up immune complexes (ICs) via Fc?Rs. … This uptake of opsonized antigen leads to robust MHC class I and class II antigen presentation, inducing both CD4+ and CD8+ T cell activation in vivo.”

4. LucFlow: A method to measure Luciferase reporter expression in single cells.
Martinez, et al.PLoS One. 2023 Oct 4;18(10):e0292410. doi: 10.1371/journal.pone.0292410. PMID: 37792610; PMCID: PMC10550117
“Luciferase reporters are widely used to study gene regulation and promoter activity in bulk cell populations. … Here, we introduce LucFlow, a novel flow cytometry-based method to measure luciferase expression at the single-cell level. … This protocol utilizes specific Fc-blocking antibodies to eliminate background signal, allowing highly sensitive and precise quantification of reporter activity in heterogeneous cell mixtures.”

5. Interrogation of Sphingosine-1-phosphate receptor 2 function in vivo reveals a prominent role in the recovery from IgE and IgG-mediated anaphylaxis with minimal effect on its onset.
Oskeritzian, et al.PLoS One. 2013 Mar 13;8(3):e58123. doi: 10.1371/journal.pone.0058123. PMID: 23516456; PMCID: PMC3602243
“Sphingosine-1-phosphate (S1P) is a bioactive lipid that regulates various physiological and pathological processes through five G protein-coupled receptors. … Using S1P2-deficient mice, we investigated the specific role of this receptor in IgE- and IgG-mediated systemic anaphylaxis. … Our results reveal that while S1P2 has minimal effect on the onset of anaphylaxis, it plays a prominent role in the recovery phase by regulating vascular permeability and cardiovascular function.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD16/CD32 Monoclonal Antibody (Clone: 2.4G2) formats, please contact our scientific support team at message@sydlabs.com.