Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone 17A2), Rat IgG2b Kappa PA007254.r2b Ushelf

Anti-mouse CD3 Antibody for Flow Cytometry | PA007254.r2b

$40.00 – $80.00

Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007254.r2b
Product Name	Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2), Rat IgG2b Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 3, CD3D, CD3E, CD3G
Summary	The recombinant anti-mouse CD3 monoclonal antibody (clone: 17A2) was produced in mammalian cells.
Clone	17A2.
Isotype	Rat IgG2b kappa.
Source/Host	The recombinant anti-mouse CD3 monoclonal antibody (clone: 17A2) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant 17A2 antibody for Flow Cytometry specifically binds to the mouse T cell receptor CD3.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2) for Flow Cytometry, Rat IgG2b Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant rat IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007254.r2b: Recombinant Anti-mouse CD3 Monoclonal Antibody (Clone: 17A2) for Flow Cytometry, Rat IgG2b Kappa

The 17A2 antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes.

References of Anti-Mouse CD3 Monoclonal Antibody (Clone: 17A2)

1. Inhibition of T cell-mediated inflammation in uveitis by a novel anti-CD3 antibody
Sugita S, Shimizu J, Makabe K, Keino H, et al.Arthritis Res Ther. 2017 Jul 25;19(1):176. PMID: 28743289
“In brief, CFSE-labeled C57BL/6JJcl splenocytes (2 × 10^6 cells/well) and irradiated (20 Gy) BALB/c splenocytes (2 × 10^5 cells/well) were cocultured in 24-well plates with 1 μg/ml of Dow2, a control anti-mouse CD3 ε Ab (clone 17A2), or an isotype control (rat IgG)…Mouse pan-T cells isolated from C57BL/6JJcl donors were incubated with 1 μg/ml of Dow2 or a control anti-mouse CD3 Ab (clone 17A2 or clone 145-2C11) at 4 °C for 30 min…Incubation with Dow2 alone or rat IgG did not stimulate T cells to produce detectable IFN-γ (Fig. 2b, left panel) or IL-17 (Fig. 2c, left panel). In contrast, T cells incubated with 17A2 (anti-mouse CD3 antibody) produced increased levels of cytokines which were significantly reduced in T cells incubated with both Dow2 and 17A2 (anti-mouse CD3 mAb)…In contrast, IRBP-stimulated production of IFN-γ was significantly enhanced in ocular cells incubated with 17A2 antibody (P < 0.005)…The expression of CD4 and CD8 on splenocytes from normal control mice and Dow2- or rat IgG-treated EAU mice was assessed by flow cytometry.”

2. In vivo Imaging Technologies to Monitor the Immune System
McCarthy CE, White JM, Viola NT, Gibson HM.Front Immunol. 2020 Jun 2;11:1067. PMID: 32582173
“Upon ex vivo analysis, one study reported a relative depletion of CD4 T cells and an expansion of CD8 T cells after delivery of an 89Zr-anti-CD3 tracer (clone 17A2) in mice…Conjugation of the contrast material to an antibody allows for specific targeting of a cell population or protein of interest…Protein platforms including antibodies, cytokines, and receptor ligands are also popular choices as molecular imaging agents for positron emission tomography (PET), single-photon emission computerized tomography (SPECT), scintigraphy, and optical imaging…Promising new adoptive cell therapies (ACT) for cancer, including ex vivo expanded tumor-infiltrating lymphocytes (TILs) and chimeric antigen receptor T cells (CAR-T cells), would benefit from imaging technologies that track cell fate in vivo…CD8 + cytotoxic T lymphocytes (CTLs) specific to the melanoma antigen Melan-A were 111In-labeled, infused in patients, and evaluated using serial whole body and static gamma camera imaging.”

3. Quantitative CD3 PET Imaging Predicts Tumor Growth Response to Anti-CTLA-4 Therapy
Larimer BM, Wehrenberg-Klee E, Caraballo A, Mahmood U.J Nucl Med. 2016 Oct;57(10):1607-1611. PMID: 27230929
“The monoclonal antimouse CD3 antibody clone 17A2 was purchased from…Conjugation and radiolabeling of the CD3 antibody resulted in a pure, highly specific probe for CD3-expressing cells…For Western blotting, the tumors were lysed in 1% sodium dodecyl sulfate and subjected to sodium dodecyl sulfate–polyacrylamide gel electrophoresis…Blots were transferred to nitrocellulose, blocked with 5% nonfat dry milk, and probed with the appropriate antibodies…Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded 10-μm slices of tumor to confirm the cellular location of CD3 expression.

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD3 Monoclonal Antibody (Clone: 17A2) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.