Description

PA007422.h1Fs: Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: FMC63) for Flow Cytometry, Human IgG1 Fc Silent Kappa, In vivo Grade

The FMC63 antibody binds to the human CD19 protein, a transmembrane protein expressed in all B lineage cells including human plasma cells. CD19 plays two major roles in human B cells: it acts as an adaptor protein to recruit cytoplasmic signaling proteins to the membrane, and works within the CD19/CD21 complex to decrease the threshold for B cell receptor signaling pathways. CD19 is a biomarker for B lymphocyte development, lymphoma diagnosis and can be utilized as a target for leukemia immunotherapies.

References of Anti-Mouse CD19 Monoclonal Antibody (Clone: 1D3)

1. Selective B cell depletion upon intravenous infusion of replication-incompetent anti-CD19 CAR lentivirus.
Muller N, et al.Mol Ther Methods Clin Dev. 2022 Jul 8;26:131-141. PMID: 35755944
“Cells were stained with FITC-conjugated anti-CD19 mAb (clone 1D3) and analyzed by flow cytometry to monitor B cell depletion. The depletion efficiency was >95% in peripheral blood as determined by flow cytometry using 1D3 antibody. Peripheral blood was collected and stained with anti-CD19 (1D3) and anti-CD3 antibodies. Flow cytometry was performed on a FACSCalibur using CellQuest software. B cell depletion was confirmed by the absence of CD19+ cells in flow analysis.”

2. Therapeutic efficacy of anti-CD19 CAR-T cells in a mouse model of systemic lupus erythematosus.
Ma J, et al.Cell Mol Immunol. 2021 Aug;18(8):1896-1903. PMID: 32472023
“For flow cytometry, cells were stained with anti-CD19 (clone 1D3) antibody to identify B cells. The percentage of CD19+ B cells was significantly reduced in treated mice. Spleen cells were isolated and stained with 1D3 antibody for CD19 expression. Data were acquired on a BD FACSCanto II flow cytometer. Treatment led to long-term B cell depletion as measured by flow.”

3. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells.
Kochenderfer JF, et al.Blood. 2010 Nov 11;116(19):3875-86. PMID: 20631379
“B cells were identified by staining with PE-conjugated anti-CD19 (clone 1D3) antibody. Flow cytometry analysis showed eradication of CD19+ cells in lymphoma. The antibody 1D3 was used to gate B cell population in bone marrow. Peripheral blood B cells were monitored weekly using 1D3. CAR-T cells specifically targeted CD19+ cells as confirmed by flow.”

4. Self-renewal of B-1 lymphocytes is dependent on CD19.
Krop I, et al.Eur J Immunol. 1996 Jan;26(1):238-42. PMID: 8566073
“Cells were stained with anti-CD19 mAb (1D3) for flow sorting. Peritoneal cells were analyzed for CD19 expression using 1D3. The antibody 1D3 was used in all flow cytometry experiments for B cell identification. Adoptive transfer experiments used sorted CD19+ cells with 1D3. B-1 cell self-renewal was assessed by limiting dilution analysis after sorting with 1D3.”

5. The signaling activity of murine CD19 is regulated during cell development.
Krop I, et al.J Immunol. 1996 Jul 1;157(1):48-56. PMID: 8683154
“Murine B cells were stained with biotinylated 1D3 anti-CD19 mAb followed by streptavidin-PE. Flow cytometry was used to assess CD19 expression levels during development. The 1D3 clone was specific for murine CD19. Developmental stages were gated based on 1D3 staining intensity. Signaling was correlated with CD19 surface density measured by 1D3.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD19 Monoclonal Antibody (Clone: 1D3) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:

Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: FMC63), Mouse IgG2a Kappa
Non-conjugated Recombinant Anti-human CD19 Monoclonal Antibody (Clone: FMC63), Human IgG1 Fc Silent Kappa

Syd Labs provides the following recombinant anti-mouse CD19 monoclonal antibodies for flow cytometry:

Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3) for flow cytometry

Syd Labs provides the following recombinant anti-human CD19 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63) for flow cytometry
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43) for flow cytometry

Syd Labs provides the following in vivo grade recombinant anti-mouse CD19 monoclonal antibodies:

Recombinant Anti-mouse CD19 monoclonal antibody (Clone: 1D3)

Syd Labs provides the following in vivo grade recombinant anti-human CD19 monoclonal antibodies:

Recombinant Anti-human CD19 monoclonal antibody (Clone: SJ25C1)
Recombinant Anti-human CD19 monoclonal antibody (Clone: B43)
Recombinant Anti-human CD19 monoclonal antibody (Clone: FMC63)

Anti-human CD19 (FMC63) for Flow Cytometry from: Anti-human CD19 Monoclonal Antibody (Clone: FMC63), Human IgG1 Fc Silent Kappa: PA007422.h1Fs Syd Labs