Anti-human CD20 Antibody for Flow Cytometry | PA007465.m2b

Catalog No.	PA007465.m2b
Product Name	Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD antigen CD20, CD20
Clone	2H7.
Isotype	Mouse IgG2b Kappa.
Source/Host	The anti-human CD20 monoclonal antibody (clone: 2H7) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant 2H7 antibody for Flow Cytometry specifically binds to mouse CD20.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7) for Flow Cytometry, Mouse IgG2b Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Mouse IgG2b isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007465.m2b: Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7)for Flow Cytometry, Mouse IgG2b Kappa, In vivo Grade

References of Anti-Human CD20 Monoclonal Antibody (Clone: 2H7)

1. IgG-Based Bispecific Anti-CD95 Antibodies for the Treatment of B Cell-Derived Malignancies and Autoimmune Diseases.
Hörner, S., et al.Cancers (Basel). 2022 Aug 12;14(16):3941. doi: 10.3390/cancers14163941. PMID: 36010934
“The study utilized hybridoma-derived antibodies 2H7, 4G7, and Apo-1 for quantification. The bispecific antibodies (bsAbs) combined the CD95 agonist Apo-1 with either the anti-CD20 antibody clone 2H7 or the anti-CD19 clone 4G7 in F(ab)2 and single-chain formats. The CD95xCD20 bsAb demonstrated remarkable potency with IC50 values as low as 25 pM, despite the relatively low affinity of the anti-CD20 mAb clone 2H7 as a single chain. Expression of CD20, CD19, and CD95 on various cell lines was quantified using flow cytometry with QIFIKIT calibration beads, and binding of bsAbs to Daudi and Jurkat cells was assessed via flow cytometry.”

2. Phenotypically distinct anti-insulin B cells repopulate pancreatic islets after anti-CD20 treatment in NOD mice.
Boldison, J., et al.Diabetologia. 2019 Sep;62(9):1669-1678. doi: 10.1007/s00125-019-04974-y. PMID: 31037349
“In this study, female VH125.hCD20/NOD mice (6–8 weeks old) were treated with either anti-hCD20 antibody (clone 2H7) or control IgG2b antibody (clone MPC-11). Over a 12-week observation period, 48% of control-treated mice developed diabetes compared to 15% of 2H7-treated mice. The study involved injecting groups of 6- to 8-week-old VH125.hCD20/NOD mice with 2H7 anti-CD20 or IgG isotype control, followed by analysis of spleen and pancreatic lymph node (PLN) tissue for anti-insulin B cells post-treatment.”

For more technical references or data sheets regarding the Anti-Human CD20 Monoclonal Antibody (Clone: 2H7) formats, please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following recombinant anti-human CD20 monoclonal antibodies for flow cytometry:

Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Mouse IgG2b Kappa
Recombinant Anti-human CD20 Monoclonal Antibody (Clone: 2H7), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-human CD20 antibodies:

Recombinant Anti-human CD20 monoclonal antibody (Clone: 2H7)
Recombinant Anti-human CD20 monoclonal antibody (Clone: B9E9)

Anti-human CD20 (2H7) for Flow Cytometry from: Anti-human CD20 Monoclonal Antibody (Clone: 2H7), PA007465.m2b Syd Labs