Recombinant Anti-human HLA-DR Monoclonal Antibody (Clone: L243), Human IgG1 Fc Silent Kappa PA007464.h1Fs Syd Labs

Anti-human HLA-DR Antibody for Flow Cytometry | PA007464.h1Fs

$45.00 – $90.00

Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007464.h1Fs
Product Name	Recombinant Anti-human HLA-DR Monoclonal Antibody (Clone: L243), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	DRA/DRB1, HLA class II histocompatibility antigen DR alpha/ DRB1-15 beta chain, HLA-DRA1/DRB1, MHC class II antigen DRA
Clone	L243.
Isotype	Human IgG1 kappa.
Source/Host	The anti-human HLA-DR monoclonal antibody (clone: L243) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant L243 antibody for Flow Cytometry specifically binds to mouse HLA-DR.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human HLA-DR Monoclonal Antibody (Clone: L243) for Flow Cytometry, Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007464.h1Fs: Recombinant Anti-human HLA-DR Monoclonal Antibody (Clone: L243) for Flow Cytometry, Human IgG1 Fc Silent Kappa, In vivo Grade

References of Anti-Human HLA-DR Monoclonal Antibody (Clone: L243)

1. Identifying major histocompatibility complex class II-DR molecules in bovine and swine peripheral blood monocyte-derived macrophages using mAb-L243
Celis-Giraldo, C., et al.Vaccine. 2024 May 31. PMID: 38631956
“The L243 monoclonal antibody (anti-human HLA-DR) was used to identify MHC-II-DR molecules in bovine and swine macrophages via flow cytometry, mass spectrometry, and immunopurification. The study demonstrated cross-reactivity of L243 with bovine and swine DR class II molecules, validating its use for immunopeptidomics to identify Staphylococcus aureus and Salmonella enterica peptides presented by MHC-II-DR.”

2. HLA class II antibodies induce necrotic cell death in human endothelial cells via a lysosomal membrane permeabilization-mediated pathway
Aljabri, A., et al.Cell Death Dis. 2019 Mar 8. PMID: 30850581
“The L243 antibody (anti-human HLA-DR) induced cell death in IFN-γ-stimulated human endothelial cells after 3 hours, as shown by annexin V/propidium iodide and LIVE/DEAD staining. Preincubation with soluble recombinant HLA-DR confirmed L243’s specific binding to HLA-DR, demonstrating its cytotoxic effect in the absence of complement, unlike its proliferative effect after 48 hours.”

3. Combating non-Hodgkin lymphoma by targeting both CD20 and HLA-DR through CD20–243 CrossMab
Zhao, L., et al.MAbs. 2014 May-Jun;6(3):740-8. PMID: 24670986
“The L243 antibody (anti-human HLA-DR) served as a positive control for HLA-DR binding in flow cytometry, showing comparable binding affinity to CD20-243 CrossMab on Raji cells. L243 competition confirmed specific HLA-DR epitope binding, with flow cytometry revealing enhanced internalization upon co-engagement of CD20 and HLA-DR.”

4. A naturally processed HLA-DR-bound peptide from the IL-9 receptor alpha of HTLV-1-transformed T cells serves as a T helper epitope
Kobayashi, H., et al.Cancer Immunol Immunother. 2012 Dec. PMID: 22638550
“The L243 antibody (anti-human HLA-DR) was used to immunoprecipitate HLA-DR molecules from HTLV-1-transformed T cells, with eluted peptides analyzed by mass spectrometry. Flow cytometry with L243 confirmed high HLA-DR surface expression, and L243 blocked T cell responses, verifying HLA-DR-restricted presentation of an IL-9 receptor alpha epitope.”

5. Characterization of a humanized IgG4 anti-HLA-DR monoclonal antibody that lacks effector cell functions but retains direct antilymphoma activity and increases the potency of rituximab
Stein, R., et al.Blood. 2006 Oct 15. PMID: 16778139
“The L243 antibody (anti-human HLA-DR), among other murine anti-HLA class II mAbs, demonstrated antibody-dependent cellular cytotoxicity (ADCC) in lymphoma cells. A humanized IgG4 version of L243 retained direct antilymphoma activity without effector functions, enhancing rituximab’s potency in targeting HLA-DR-expressing cells.”

For more technical references or data sheets regarding the Recombinant Anti-Human HLA-DR Monoclonal Antibody (Clone: L243) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.