Recombinant Anti-human HLA-DRDPDQ Monoclonal Antibody (Clone F3.3), Human IgG1 Fc Silent Kappa PA007471.h1Fs Syd Labs

Anti-human HLA-DR/DP/DQ Antibody for Flow Cytometry | PA007471.h1Fs

$100.00 – $200.00

Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007471.h1Fs
Product Name	Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	beta1 domain MHC class II HLA DPB, CELIAC1, class II HLA beta chain, MHC class II HLA-DR, DP, DQ
Clone	F3.3.
Isotype	Human IgG1 kappa.
Source/Host	The anti-human HLA-DR/DP/DQ monoclonal antibody (clone: F3.3) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant F3.3 antibody for Flow Cytometry specifically binds to mouse HLA-DR/DP/DQ.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3) for Flow Cytometry, Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007471.h1Fs: Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3) for Flow Cytometry, Human IgG1 Fc Silent Kappa, In vivo Grade

References of Anti-Human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3)

1. HLA antibody affinity determination: From HLA-specific monoclonal antibodies to donor HLA specific antibodies in transplantation.
Hug, K., et al.HLA. 2023 Jul;102(1):3-15. doi: 10.1111/tan.14876. PMID: 37191252
“The F3.3 antibody (anti-human HLA-DR/DP/DQ) was used to assess HLA-DR expression on donor lymphocytes via flow cytometry, with PE-conjugated F3.3 staining cells for 30 minutes on ice. Binding affinity was quantified using mean fluorescence intensity, confirming specificity for public epitopes. Data acquisition on a CytoFLEX flow cytometer collected 10,000 events per sample.”

2. Recombinant human monoclonal HLA antibodies of different IgG subclasses recognising the same epitope: Excellent tools to study differential effects of isotypes in antibody-dependent cellular cytotoxicity.
Hiemstra, I. H., et al.HLA. 2019 Nov;94(5):441-450. doi: 10.1111/tan.13664. PMID: 31403241
“Recombinant F3.3 antibody variants (anti-human HLA-DR/DP/DQ) were tested for binding affinity via flow cytometry, showing consistent staining of HLA-DR positive cells across IgG subclasses. Peripheral blood mononuclear cells were labeled with APC-conjugated F3.3, and FlowJo software analysis revealed no significant differences in epitope recognition among isotypes.”

3. Production and characterization of chimeric anti-HLA monoclonal antibodies targeting public epitopes as tools for standardizations of the anti-HLA antibody detection.
Haskova, Z., et al.Hum Immunol. 2013 May;74(5):622-9. doi: 10.1016/j.humimm.2013.01.004. PMID: 23352853
“Chimeric monoclonal antibodies, including F3.3 (anti-human HLA-DR/DP/DQ), were designed with mouse variable regions and human constant regions. The chimeric F3.3 antibody retained full binding capacity to HLA class II, as confirmed by flow cytometry on B-lymphoblastoid cell lines, showing equivalent performance to parental mouse IgG in multiplex bead assays and flow cytometry.”

4. Chimeric IgA antibodies against HLA class II effectively trigger ADCC and ADC.
Brüggemann, M., et al.Blood. 2007 Feb 1;109(3):1141-8. doi: 10.1182/blood-2006-06-029017. PMID: 17202363
“Chimeric F3.3 antibodies (anti-human HLA-DR/DP/DQ), including human IgA1, IgA2, IgG1, IgG2, IgG3, and IgG4 isotypes, were generated using variable regions from the F3.3 hybridoma. Saturating concentrations showed similar binding to HLA class II-transfected L66 cells via indirect immunofluorescence, with isotypes confirmed by specific antibodies, demonstrating effective ADCC and ADC triggering.”

For more technical references or data sheets regarding the Anti-Human HLA-DR/DP/DQ Monoclonal Antibody (Clone: F3.3) formats, please contact our scientific support team at message@sydlabs.com.