Anti-Human HLA Class I Heavy Chain Antibody for Flow Cytometry | PA007483.h1Fs

Catalog No.	PA007483.h1Fs
Product Name	Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: HC10)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA), HLA class I histocompatibility antigen
Clone	HC10.
Isotype	Human IgG1 kappa.
Source/Host	The anti-human HLA Class I Heavy Chain monoclonal antibody (clone: HC10) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant HC10 antibody for Flow Cytometry specifically binds to human HLA Class I Heavy Chain.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody for Flow Cytometry, Human IgG1 Fc Silent Kappa (Clone: HC10) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant human IgG1 isotype controls is available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007483.h1Fs: Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody (Clone: HC10) for Flow Cytometry, Human IgG1 Fc Silent Kappa, In vivo Grade

References of Anti-Human HLA Class I Heavy Chain Monoclonal Antibody (Clone: HC10)

1. Endo-lysosomal assembly variations among human leukocyte antigen class I allotypes
Olson, E., et al.Elife. 2023;12:e79144. PMID: 36722462
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) was used in peptide receptivity assays to measure specific expression and localization of HLA-B allotypes in donors selected from a genotyped cohort. Anti-Bw6 and anti-Bw4 antibodies, including HC10, facilitated analysis of homozygous or heterozygous HLA-B allotypes, ensuring minimal cross-reactivity with HLA-A or HLA-C allotypes.”

2. Non-conventional forms of HLA-B27 are expressed in spondyloarthritis joints and gut tissue
Rysnik, O., et al.J Autoimmun. 2016;70:12-21. PMID: 27036372
“The HC10 antibody (anti-human HLA Class I Heavy Chain) and HD6 were used to detect non-conventional HLA-B27 (NC-B27) expression in human SpA joints, gut, and B27 TG 1 rat tissues via immunohistochemistry, flow cytometry, and confocal microscopy. High NC-B27 levels were observed in infiltrating and resident cells of inflamed joints and spleens of B27 TG 1 rats, increasing with age and disease progression.”

3. HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats
Marroquin Belaunzaran, O., et al.PLoS One. 2015;10(6):e0130811. PMID: 26125554
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain, IgG2a) served as a positive control, binding β2m-free heavy chains of HLA-B and -C, including B272 and B27-β2m-free forms. Flow cytometry with HC10, HD6, and other antibodies (0.02 mg/mL) on LBL721.220 cells and leukocytes from transgenic rats confirmed cell-surface B272 expression, with secondary detection using PE-conjugated goat anti-mouse IgG.”

4. The Leukocyte Immunoglobulin-Like Receptor Family Member LILRB5 Binds to HLA-Class I Heavy Chains
Jones, D. C., et al.PLoS One. 2015;10(6):e0129063. PMID: 26098415
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) blocked B27 dimer tetramer binding to LILRB5-transfected 293T cells, as confirmed by flow cytometry with W6/32, HC10, and ME1 antibodies. HC10 and anti-LILRB5 antisera inhibited B27 dimer binding, while multiparameter flow cytometry assessed LILRB5 surface expression on lymphocyte and monocyte populations.”

5. Psoriatic patients with arthropathy show significant expression of free HLA class I heavy chains on circulating monocytes: a potential role in the pathogenesis of psoriatic arthropathy
Lan, C-C E, et al.Br J Dermatol. 2004;151(1):24-31. PMID: 15270869
“The HC10 monoclonal antibody (anti-human HLA Class I Heavy Chain) detected free HLA class I heavy chains on circulating monocytes via flow cytometry in patients with psoriatic arthropathy. Peripheral blood mononuclear cells, isolated by Ficoll-Hypaque centrifugation, were stained with HC10 followed by FITC-conjugated goat anti-mouse IgG and analyzed using a FACScan flow cytometer with CellQuest software.”

For more references about anti-human HLA Class I Heavy Chain antibody (HC10), please contact our scientific support team at message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human HLA monoclonal antibodies:

In vivo grade recombinant anti-human HLA-ABC monoclonal antibodies (clone W6/32)
In vivo grade recombinant anti-human HLA-DR monoclonal antibodies (clone L243)
In vivo grade recombinant anti-human HLA-DR/DP/DQ monoclonal antibodies (clone F3.3)
In vivo grade recombinant anti-human HLA class II DR/DQ monoclonal antibodies (clone 9.3F10)
In vivo grade recombinant anti-human HLA Class I Heavy Chain monoclonal antibodies (clone HC10)

Syd Labs provides the following recombinant anti-human HLA monoclonal antibodies for flow cytometry:

Recombinant anti-human HLA-ABC monoclonal antibodies (clone W6/32) for flow cytometry
Recombinant anti-human HLA-DR monoclonal antibodies (clone L243) for flow cytometry
Recombinant anti-human HLA-DR/DP/DQ monoclonal antibodies (clone F3.3) for flow cytometry
Recombinant anti-human HLA class II DR/DQ monoclonal antibodies (clone 9.3F10) for flow cytometry
Recombinant anti-human HLA Class I Heavy Chain monoclonal antibodies (clone HC10) for flow cytometry

Anti-Human HLA Class I Heavy Chain ( HC10) for Flow Cytometry from: Recombinant Anti-Human HLA Class I Heavy Chain Monoclonal Antibody, Human IgG1 Fc Silent Kappa (Clone: HC10): PA007483.h1Fs Syd Labs