Anti-human HLA-ABC Antibody for Flow Cytometry | PA007472.m2a

Catalog No.	PA007472.m2a
Product Name	Recombinant Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32), Mouse IgG2a Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA), HLA class I histocompatibility antigen
Clone	W6/32.
Isotype	Mouse IgG2a Kappa.
Source/Host	The anti-human HLA-ABC monoclonal antibody (clone: W6/32) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant W6/32 antibody for Flow Cytometry specifically binds to mouse HLA-ABC.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32) for Flow Cytometry, Mouse IgG2a Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Mouse IgG2a isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007472.m2a: Recombinant Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32) for Flow Cytometry, Mouse IgG2a Kappa, In vivo Grade

References of Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32)

1. Mass spectrometric profiling of HLA-B44 peptidomes provides evidence for tapasin-mediated tryptophan editing
Kaur A, et al.J Immunol. 2023. PMID: 37737643
“This study uses mass spectrometry to explore tapasin-mediated tryptophan editing in HLA-B44 peptidomes. The W6/32 antibody (anti-human HLA-ABC) was purified from mouse ascites and crosslinked to PureProteome Protein A magnetic beads for immunoaffinity purification. Cells were stained with W6/32-FITC antibody for surface HLA class I analysis via quantitative flow cytometry, revealing a significant reduction in HLA-B44:05 molecules on tapasin knockout cells compared to wild-type cells. A slight, non-significant decrease in HLA-B44:05 surface stability was observed in tapasin knockout cells in the presence of brefeldin A.”

2. Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins
Zhang F, et al.Proc Natl Acad Sci U S A. 2022. PMID: 36136978
“This study investigates how sarbecovirus ORF7a proteins inhibit MHC-I antigen presentation. Human 293T, HuH7.5, U2OS, and A549/ACE2 cells were transduced with lentiviral vectors expressing SARS-CoV-2 ORF7a or Nef and stained with the pan-HLA-ABC antibody W6/32 for flow cytometry. MHC-I surface levels, measured by median fluorescence intensity, were reduced in ORF7a-expressing cells. The study compared wild-type and ORF7-deleted SARS-CoV-2 strains, confirming ORF7a’s role in suppressing MHC-I expression.”

3. Assessments of HLA-I Specificities of Anti-HLA-I Monoclonal Antibodies Using Solid Phase Bead Arrays
Zaitouna AJ, et al.Bio Protoc. 2020. PMID: 33659325
“This study evaluates anti-HLA-I monoclonal antibody specificities using solid phase bead arrays. The W6/32 antibody, a pan anti-HLA class I antibody, was used to normalize HLA-I protein levels on Labscreen and FlowPRA Single Antigen Beads. Biotinylated W6/32 was tested at various dilutions (undiluted to 1:100) alongside anti-Bw4 and anti-Bw6 antibodies. Representative data showed W6/32 binding to HLA-I beads, with isotype control IgG2a used for comparison.”

4. Strategies for the measurements of expression levels and half-lives of HLA class I allotypes
Raghavan M, et al.Hum Immunol. 2019. PMID: 30735755
“This study describes methods for measuring HLA class I expression and half-lives using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry histograms showed W6/32 signals for monocytes, NK cells, B cells, CD8+ T cells, and CD4+ T cells, with geometric mean values quantifying HLA class I expression. The W6/32 antibody was chosen for its broad recognition of most HLA class I allotypes, facilitating consistent assessment across healthy donors.”

5. Higher human lymphocyte antigen class I expression in early-stage cancer cells leads to high sensitivity for cytotoxic T lymphocytes
Akazawa Y, et al.Cancer Sci. 2019. PMID: 30973665
“This study examines HLA class I expression in early-stage hepatocellular carcinoma (HCC) using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry analysis of tissue from five HCC patients compared HLA class I expression between cancer cells and non-tumorous hepatocytes. The mean fluorescence intensity ratio of W6/32 staining indicated higher HLA class I expression in cancer cells, enhancing sensitivity to cytotoxic T lymphocytes. Heterogeneous expression was observed in non-tumorous hepatocytes in one case.”

For more technical references or data sheets regarding the Recombinant Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant anti-human HLA-ABC monoclonal antibodies for flow cytometry:

Recombinant Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32), Mouse IgG2a Kappa
Recombinant Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32), Human IgG1 Fc Silent Kappa

Syd Labs provides the following in vivo grade recombinant anti-human HLA-ABC monoclonal antibodies:

Recombinant Anti-human HLA-ABC monoclonal antibody (Clone: W6/32)

Anti-human HLA-ABC ( W6/32 ) for Flow Cytometry from : Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32), Mouse IgG2a Kappa: PA007472.m2a Syd Labs