Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Human IgG1 Fc Silent Kappa PA007474.h1Fs Syd Labs

Anti-human CD34 Antibody for Flow Cytometry | PA007474.h1Fs

$100.00 – $200.00

Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007474.h1Fs
Product Name	Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	CD34; CD34 antigen; CD34 molecule
Clone	My10.
Isotype	Human IgG1 kappa.
Source/Host	The anti-human CD34 monoclonal antibody (clone: My10) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant My10 antibody for Flow Cytometry specifically binds to mouse CD34.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10) for Flow Cytometry, Human IgG1 Fc Silent Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007474.h1Fs: Recombinant Anti-human CD34 Monoclonal Antibody (Clone: My10) for Flow Cytometry, Human IgG1 Fc Silent Kappa, In vivo Grade

References of Anti-Human CD34 Monoclonal Antibody (Clone: My10)

1. Generation and Characterization of Anti-CD34 Monoclonal Antibodies that React with Hematopoietic Stem Cells
Maleki LA, et al.Cell J. 2014 Spring;15(4):292-300. PMID: 24611141
“The My10 monoclonal antibody, recognizing CD34, was tested for specificity via ELISA and Western blotting, with clone 3D5 showing strong reactivity to CD34 peptides and native CD34 from human umbilical cord blood cells. Subisotyping confirmed its immunoglobulin class, and it enabled identification and enrichment of hematopoietic stem/progenitor cells.”

2. Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies
Civin CI, et al.Blood. 1987 Nov;70(5):1316-23. PMID: 3489673
“Two-color cell sorting with My10 and HLA-DR antibodies enriched hematopoietic progenitors in bone marrow, with the My10-positive, HLA-DR-positive fraction containing nearly all CFU-GM and BFU-E. Flow cytometry and progenitor assays confirmed high purity and enrichment of progenitor cells using My10.”

3. Humanization of an anti-CD34 monoclonal antibody by complementarity-determining region grafting based on computer-assisted molecular modelling
Queen C, et al.Cancer Res. 1992 Dec 15;52(24 Suppl):6761s-6767s. PMID: 1458463
“The My10 anti-CD34 antibody was humanized via CDR grafting, retaining binding affinity to CD34+ KG1 cells in flow cytometry. Comparative analysis showed similar reactivity between parental and humanized versions, with molecular modeling guiding framework region selection for humanization.”

4. Targeting retroviral vectors to CD34-expressing cells: binding to CD34 does not catalyze virus-cell fusion
Kasahara N, et al.Hum Gene Ther. 1999 Mar 20;10(5):937-46. PMID: 10094198
“The My10 scFv, expressed on retroviral vectors, targeted CD34+ KG1 cells, with flow cytometry confirming specific binding. While targeting improved transduction specificity, CD34 engagement did not enhance virus-cell fusion, as assessed in post-binding assays.”

5. My10, a new myeloid cell antigen recognized by a monoclonal antibody
Civin CI, et al.J Exp Med. 1983 Oct 1;158(4):1125-36. PMID: 6606698
“The My10 monoclonal antibody recognized a 115-kD myeloid antigen on blasts and progenitors, absent on mature granulocytes, as shown by flow cytometry. Sorting with My10 enriched CFU-GM, and immunoprecipitation confirmed the antigen’s molecular weight, establishing its utility in myeloid cell identification.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD34 Monoclonal Antibody (Clone: My10) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.