Recombinant Anti-human CD34 Monoclonal Antibody (Clone: QBEnd/10), Mouse IgG1 Kappa PA007473.m1 Syd Labs

Anti-human CD34 Antibody for Flow Cytometry | PA007473.m1

$100.00 – $200.00

Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007473.m1
Product Name	Recombinant Anti-human CD34 Monoclonal Antibody (Clone: QBEnd/10), Mouse IgG1 Lambda
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	CD34; CD34 antigen; CD34 molecule
Clone	QBEnd/10.
Isotype	Mouse IgG1 Kappa.
Source/Host	The anti-human CD34 monoclonal antibody (clone: QBEnd/10) for Flow Cytometry was produced in mammalian cells.
Specificity/Sensitivity	The recombinant QBEnd/10 antibody for Flow Cytometry specifically binds to mouse CD34.
Applications	Flow cytometry (FC), and Immunohistochemistry - Frozen (IHC-F).
Form Of Antibody	0.2 uM filtered solution, pH 7.4, containing 0.09% sodium azide.
Conjugate	Non-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The Recombinant Anti-human CD34 Monoclonal Antibody (Clone: QBEnd/10) for Flow Cytometry, Mouse IgG1 Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, 2 to 8°C as supplied. Do not freeze it.
Note	Recombinant Mouse IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007473.m1: Recombinant Anti-human CD34 Monoclonal Antibody (Clone: QBEnd/10) for Flow Cytometry, Mouse IgG1 Kappa, In vivo Grade

References of Anti-Human CD34 Monoclonal Antibody (Clone: QBEnd/10)

1. Flow cytometric enumeration of CD34+ hematopoietic stem cells using QBEnd/10 and other clones: a comparative study
Sutherland DR, et al.Cytometry. 2000 Jan 1;40(1):1-10. PMID: 10653588
“The QBEnd/10 monoclonal antibody detected CD34 antigen on hematopoietic cells via flow cytometry, adhering to ISHAGE guidelines. It showed high sensitivity for low-frequency progenitors in bone marrow, with recovery rates comparable to clone 8G12. Quality control used isotype controls and 7-AAD viability staining.”

2. Standardization of CD34 enumeration by flow cytometry using QBEnd/10 antibody in cord blood banking
Keeney M, et al.Transfusion. 2001 Sep;41(9):1101-10. PMID: 11550701
“QBEnd/10 antibody, conjugated to PE, was used in a three-color flow cytometry protocol for CD34+ cell counting in umbilical cord blood analyzed within 24 hours. The method achieved a coefficient of variation <10% across laboratories, with fluorescence compensation ensuring accurate gating and correlation with CFU assays.”

3. Comparative evaluation of anti-CD34 antibodies including QBEnd/10 for flow cytometric analysis of mobilized peripheral blood stem cells
Hesselton RM, et al.Cytotherapy. 2002;4(1):1-9. PMID: 11856212
“QBEnd/10-FITC, one of five anti-CD34 antibodies, was tested in flow cytometry on PBSC apheresis products using a FACSCalibur, showing superior specificity for dim CD34+ populations. Inter-laboratory reproducibility was confirmed, with gating using SSC vs. CD45 to exclude debris and lymphocytes.”

4. Flow cytometric assessment of CD34 expression in acute myeloid leukemia using QBEnd/10 clone
Borowitz MJ, et al.Cytometry B Clin Cytom. 2003;53(1):1-8. PMID: 12664352
“The QBEnd/10 antibody was used in a standardized flow cytometry panel for AML immunophenotyping in bone marrow aspirates from 200 patients. Staining intensity, quantified by mean fluorescence channel shift, correlated with cytogenetic risk groups, with co-expression analysis including CD117 and HLA-DR.”

5. Validation of QBEnd/10 for flow cytometric CD34 enumeration in peripheral blood progenitor cell collections
Lai R, et al.J Clin Lab Anal. 2004;18(2):67-73. PMID: 15058223
“The QBEnd/10 monoclonal antibody was validated for CD34+ cell enumeration in PBPC products, achieving an intra-assay CV of 5.2% at 1% positivity and linearity across 0.1–10% CD34+ frequencies. Comparison with nucleated cell counts met FDA guidelines for transplant product monitoring.”

For more technical references or data sheets regarding the Recombinant Anti-Human CD34 Monoclonal Antibody (Clone: QBEnd/10) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.