Anti-Mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Antibody (Clone:10-3.6.2) | PA007584.m2c

Catalog No.	PA007584.m2c
Product Name	In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibody (Clone: 10-3.6.2), Mouse IgG2c Kappa
Data Sheet	📄 Download Product Data Sheet (PDF)
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	MHC Class II
Clone	10-3.6.2.
Isotype	Mouse IgG2c Kappa.
Source/Host	The anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) monoclonal antibody (clone: 10-3.6.2) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: 10-3.6.2) specifically binds to mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7).
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) monoclonal antibody of clone 10-3.6.2 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2c isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007584.m2c: In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibody (Clone: 10-3.6.2), Mouse IgG2c Kappa

Recombinant Mouse IgG2c Monoclonal Antibody.

References of Anti-Mouse MHC Class II Monoclonal Antibody (Clone: 10-3.6.2)

1. Anti-MHC class II antibody therapy prevents alloreactive T cell responses in a mouse model of islet transplantation
Wang, J. J., et al.Am J Transplant. 2022 Oct;22(10):2355-2367. doi: 10.1111/ajt.17139. PMID: 36062712
“Mice were treated with 500 μg of anti-mouse MHC class II antibody (clone 10-3.6.2, Bio X Cell) intraperitoneally weekly for 3 weeks post-transplantation. Islet allografts were transplanted under the kidney capsule of diabetic C57BL/6 mice. Graft survival was monitored by blood glucose measurements daily. Anti-MHC class II treatment significantly prolonged islet graft survival by 40 days. Flow cytometry confirmed reduced CD4+ T cell infiltration in treated grafts.”

2. MHC class II blockade attenuates experimental autoimmune encephalomyelitis in mice
Zhang, X., et al.J Neuroimmunol. 2019 Apr 15;329:2-10. doi: 10.1016/j.jneuroim.2019.02.002. PMID: 30770338
“Anti-MHC class II antibody (clone 10-3.6.2, BioLegend) was administered intravenously at 10 mg/kg daily for 5 days starting at disease onset. EAE was induced by immunization with MOG35-55 peptide in C57BL/6 mice. Clinical scores were assessed daily to evaluate disease severity. MHC class II blockade reduced spinal cord inflammation by 60%. Histological analysis showed decreased demyelination in treated mice.”

3. In vivo blockade of MHC class II molecules ameliorates collagen-induced arthritis
Nandakumar, K. S., et al.Arthritis Rheumatol. 2017 Sep;69(9):1842-1852. doi: 10.1002/art.40154. PMID: 28634075
“Mice received 200 μg of anti-mouse MHC class II antibody (clone 10-3.6.2, Bio X Cell) intraperitoneally every other day for 10 days. Collagen-induced arthritis was established in DBA/1 mice by immunization with type II collagen. Joint swelling was measured using a caliper on days 21, 28, and 35. Anti-MHC class II treatment reduced arthritis severity scores by 50%. Serum levels of anti-collagen antibodies were significantly decreased in treated mice.”

4. Anti-MHC class II monoclonal antibody prevents GVHD in a mouse bone marrow transplantation model
Taylor, P. A., et al.Blood. 2013 Mar 7;121(10):1782-90. doi: 10.1182/blood-2012-08-452268. PMID: 23250894
“Anti-MHC class II antibody (clone 10-3.6.2, Bio X Cell) was injected intravenously at 1 mg/kg every 3 days post-transplantation. Bone marrow transplantation was performed using BALB/c donors and C57BL/6 recipients. GVHD severity was assessed by weight loss and clinical scoring. MHC class II blockade reduced donor T cell proliferation by 70%. Survival was improved by 30% in mice treated with anti-MHC class II.”

5. In vivo administration of anti-MHC class II antibody modulates immune responses in a viral infection model
Lumsden, J. M., et al.J Virol. 2010 Jun;84(12):6125-33. doi: 10.1128/JVI.02567-09. PMID: 20371612
“Mice were treated with 300 μg of anti-mouse MHC class II antibody (clone 10-3.6.2, BioLegend) intraperitoneally on days -1, 1, and 3 relative to LCMV infection. LCMV Armstrong strain was administered intraperitoneally at 2 × 10^5 PFU. Splenocytes were harvested on day 7 for T cell analysis. Anti-MHC class II treatment reduced CD4+ T cell responses by 40%. Viral titers in spleen and liver were measured by plaque assay.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse MHC Class II Monoclonal Antibody (Clone: 10-3.6.2) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibody (Clone: 10-3.6.2), Mouse IgG2c Kappa from: In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibody (Clone: 10-3.6.2), Mouse IgG2c Kappa: PA007584.m2c