Anti-Mouse CD209b (SIGN-R1) Antibody (Clone:22D1) | PA007590.m2a

Description

PA007590.m2a: In Vivo Grade Recombinant Anti-mouse CD209b (SIGN-R1) Monoclonal Antibody (Clone: 22D1), Mouse IgG2a Kappa

Recombinant Mouse IgG2a Monoclonal Antibody.

References of Anti-Mouse CD209b (SIGN-R1) Monoclonal Antibody (Clone: 22D1)

1. PD-1 promotes immune exhaustion by inducing antiviral T cell motility paralysis.
Zinselmeyer, B. H., et al.J Exp Med. 2013 Apr 8;210(4):757-74. doi: 10.1084/jem.20121416. PMID: 23530125
“After the Fc block, cells were stained with cocktails of the following conjugated antibodies: CD279 biotin (RMP1-30), CD274 biotin (10F.962), F4/80 PerCP (BMB), CD45.2 APC (104), CD11c APC Cy7 (N418), CD45.1 Alexa Fluor 700 (A20), CD45.2 Alexa Fluor 700 (104; BioLegend), CD4 FITC (RM4-5), Thy1.1 PerCP (OX-7), CD8 PE (53–6.7), Thy1.2 PE (53–2.1; BD), CD11b PE Cy7 (M1/70), CD209b Alexa Fluor 647 (22D1; eBioscience), CD169 FITC (3D6.112; Serotec), CD8 Pacific Blue (5H10; Invitrogen), and gp38 PE (8.1.1; BioLegend). …(A and B) The geometric mean fluorescent intensity (GMFI) of PD-L1 expression (mean ± SD) was quantified on splenic marginal zone macrophages (CD45+ Thy1.2− F4/80− CD209b+; MZM), myeloid dendritic cells (CD45+ Thy1.2− F4/80− CD169− CD209b− CD11c+ CD11b+; mDCs), red pulp macrophages (CD45+ Thy1.2− CD169− CD209b− CD11c− CD11b+ F4/80+; red pulp Mϕ), metallophilic macrophages (CD45+ Thy1.2− F4/80− CD169+; MMM), CD8α DCs (CD45+ Thy1.2− F4/80− CD169− CD209b− CD11b− CD11c+ CD8α+), CD8 T cells (CD45+ Thy1.2+ CD8α+), and CD4 T cells (CD45+ Thy1.2+ CD4+) at days 4 and 7 after Arm or CL13 infection. …Spleens were homogenized and subsequently treated with TRIzol before performing a column-based RNA purification using the PureLink RNA mini kit (Invitrogen). …The reactions were performed in triplicate, and RNA samples without reverse transcription were used as a negative control for each gene candidate. …After blocking with 5% casein, ICAM-1-12-His labeled with Alexa Fluor 405 (AF405; Invitrogen; 200 molecules/µm2) was reacted with lipid bilayers in combination with H-2DbGP33-41-6-His (NIH tetramer core facility, Emory University; 185 molecules/um2).”

2. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen.
Kang, Y. S., et al.Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):215-20. doi: 10.1073/pnas.0307124101. PMID: 14694198
“To establish a role for SIGN-R1 in this uptake, we selectively down-regulated expression of the lectin by pretreatment of the mice with SIGN-R1 antibodies, including a newly generated hamster monoclonal called 22D1. …A soluble SIGN-R1 antigen of fusion between the extracellular portion of SIGN-R1 and mouse IgG Fc was produced, affinity purified from transfected mammalian cells, and used as antigen to generate a new hamster monoclonal antibody, 22D1, in the Hybridoma Core Facility at Mt. Sinai School of Medicine. …After immunization of hamsters with SIGN-R1 fusion protein, we retrieved a stable monoclonal, named 22D1, that selectively bound to SIGN-R1 but not to cells transfected with other lectins (data not shown). …The 22D1 antibody also immunoblotted SIGN-R1 (see below). However, the hamster antibody recognized a site distinct from the peptide recognized by rabbit PAb-C13 antibody. …Similar results were obtained by FACS analysis, i.e., staining with 22D1 did not block staining with PAb-C13, and staining with PAb-C13 did not block reactivity with 22D1.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD209b (SIGN-R1) Monoclonal Antibody (Clone: 22D1) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse CD209b (SIGN-R1) Monoclonal Antibody (Clone: 22D1), Mouse IgG2a Kappa from: In Vivo Grade Recombinant Anti-mouse CD209b (SIGN-R1) Monoclonal Antibody (Clone: 22D1), Mouse IgG2a Kappa: PA007590.m2a