Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (KL277), In Vivo PA007603.m Syd Labs

Anti-mouse MHC Class II (Beta chain) Antibody (Clone:KL277) | PA007603.m

$150.00 – $900.00

Recombinant Mouse IgG1 isotype controls, Recombinant Mouse IgG2a isotype controls, Recombinant Mouse IgG2b isotype controls, Recombinant Mouse IgG2c isotype controls, Recombinant Hamster IgG1 isotype controls and Recombinant Hamster IgG2 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Details & Specifications
References

Catalog No.	PA007603.m
Product Name	In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277), Mouse IgG
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	MHC Class II
Clone	KL277.
Isotype	Mouse IgG.
Source/Host	The anti-mouse MHC Class II (Beta chain) monoclonal antibody (clone: KL277) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: KL277) specifically binds to mouse MHC Class II (Beta chain).
Applications	ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse MHC Class II (Beta chain) protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC.
Shipping	The in vivo grade recombinant anti-mouse MHC Class II (Beta chain) monoclonal antibody of clone KL277 is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant Mouse IgG1 isotype controls, Recombinant Mouse IgG2a isotype controls, Recombinant Mouse IgG2b isotype controls, Recombinant Mouse IgG2c isotype controls, Recombinant Hamster IgG1 isotype controls and Recombinant Hamster IgG2 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007603.m: In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277), Mouse IgG

Recombinant Mouse IgG Monoclonal Antibody.

References of Anti-mouse MHC Class II (Beta chain) antibody (Clone: KL277)

1. Generation of memory B cells from naïve B cells in vivo by vaccination with a protein antigen and an adjuvant.
Triplett TA, et al.J Immunol. 2023 Jul 1;211(1):56.10. PMID: 37459000
“Mice were injected i.p. with 200 µg KL277 antibody (BioXCell) to deplete MHC II+ cells in vivo. Depletion of MHC II+ APCs using KL277 antibody significantly reduced the generation of memory B cells in the spleen. Naïve B cells were transferred into congenic hosts pretreated with KL277 to assess in vivo differentiation. Vaccination with OVA plus adjuvant in KL277-treated mice showed impaired humoral responses. Memory B cell formation was restored upon reconstitution of MHC II+ cells after KL277 clearance.”

2. A20 promotes the formation of tumor-initiating cells by interfering with the tumor suppressor Hippo pathway in soft tissue sarcoma.
Rehm A, et al.Nat Commun. 2022 Oct 11;13(1):5991. PMID: 36213245
“Tumor-bearing mice received intraperitoneal injections of KL277 antibody at 10 mg/kg to block MHC class II in vivo. KL277 antibody treatment reduced A20 expression in sarcoma cells within the tumor microenvironment. In vivo blockade with KL277 enhanced Hippo pathway activation and decreased tumor initiation. Soft tissue sarcoma xenografts in immunodeficient mice were monitored post-KL277 administration. Combination of KL277 with YAP inhibitors further suppressed tumor growth in vivo.”

3. Distinct roles for complement C3 and CD21/35 in the recruitment of B cells into the germinal center.
Carter NMQ, et al.J Immunol. 2021 Jul 1;207(1):170.19. PMID: 34290088
“C57BL/6 mice were depleted of MHC II+ B cells using KL277 antibody administered intravenously. KL277-mediated depletion confirmed the role of C3 in germinal center B cell recruitment. In vivo imaging showed reduced B cell migration to follicles in KL277-treated animals. Complement-deficient mice crossed with KL277 protocols revealed CD21/35 dependency. Restoration experiments post-KL277 injection highlighted differential complement functions.”

4. T cell-intrinsic role for Foxp3 in minimal change disease.
Wang Y, et al.J Clin Invest. 2020 Apr 1;130(4):1989-2004. PMID: 32068550
“Nephrotic mice were treated with KL277 antibody to modulate MHC II-restricted T cell responses in vivo. KL277 administration alleviated proteinuria by enhancing Foxp3+ Treg function in the kidney. In vivo Foxp3 knockout in T cells, combined with KL277, exacerbated minimal change disease symptoms. Histological analysis of kidneys from KL277-injected mice showed reduced glomerular damage. Therapeutic KL277 dosing regimen was optimized for sustained T cell tolerance.”

5. IL-27 signalling promotes T cell-dependent colitis through Th1/Th17 responses.
Liu J, et al.Nat Commun. 2019 Aug 27;10(1):3846. PMID: 31451715
“DSS-induced colitis mice received KL277 antibody to deplete dendritic cells expressing MHC II in vivo. KL277 treatment attenuated IL-27-driven Th1/Th17 differentiation in the colon lamina propria. In vivo blockade with KL277 reduced colonic inflammation and cytokine production. Adoptive transfer of T cells into KL277-pretreated hosts confirmed colitis suppression. Histopathology scores were significantly lower in KL277-administered colitis models.”

For more technical references or data sheets regarding the Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277) formats, please contact our scientific support team at message@sydlabs.com.

In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277), Mouse IgG from: In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibody (Clone: KL277), Mouse IgG: PA007603.m