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Mouse IgG1 Isotype Control Antibody | PA007126

Name: Mouse IgG1 Isotype Control (12B9)
Brand: Syd Labs
SKU: PA007126
Price: 150 - 800 USD
Availability: InStock
Rating: 5 (1 reviews)

$150.00 – $800.00

Recombinant mouse IgG1 kappa isotype control and mIgG1 D265A isotype control mutant for in vitro and in vivo studies. Low or no specific binding to mouse samples tested. More choices of recombinant mouse IgG1 isotype controls, including targets, mutants, tags, and conjagates.

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Syd Labs, Inc.

SKU: PA007126 Categories: Antibodies, Antibody Isotype Controls Tags: Mouse IgG1 Isotype Control, Mouse IgG1 Isotype Control Antibody

Details & Specifications
References

Catalog No.	PA007126
Product Name	Mouse IgG1 Isotype Control Antibody \| PA007126
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Recombinant Mouse IgG1 Isotype Control, Mouse IgG1 Negative Control Antibody, mIgG1 Isotype Control
Summary	The in vivo grade recombinant mouse IgG1 isotype control antibody (mIgG1 isotype control) was produced in CHO cells.
Isotype	mouse IgG1, kappa
Applications	an isotype-matched negative control for mouse IgG1 antibody used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry
Immunogen	N/A.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The recombinant mouse IgG1 isotype control and mutants are shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007126: Recombinant Mouse IgG1 Isotype Control Antibody(Clone: 12B9), In Vivo Grade

The Mouse IgG1 Isotype Control Antibody (12B9) is a must-have for researchers seeking to ensure experimental accuracy in immunological studies. Designed as a negative control, it distinguishes specific antibody binding from non-specific background signals in critical applications like Flow Cytometry, ELISA, Immunohistochemistry (IHC-P, IHC-F), and Western Blot. Available from trusted suppliers like us, this recombinant antibody matches Mouse IgG1 kappa primary antibodies and shows no reactivity to mouse, human, or rat proteins, making it ideal for validating experiments with human PBMCs or mouse splenocytes. With high purity (>95% by SDS-PAGE) and low endotoxin levels (<0.1 EU/µg), it delivers reliable results in both in vivo and in vitro research, encouraging researchers to trust its performance in sensitive assays.

Researchers value tools that streamline workflows and ensure reproducibility, and this antibody excels with its recombinant production in mammalian cells (CHO or HEK293), offering unmatched lot-to-lot consistency compared to hybridoma-derived alternatives. Available in versatile formats—purified, FITC, PE, APC conjugates, and Fc-silenced variants—it supports diverse needs, from flow cytometry isotype control for precise staining to IHC for tissue analysis. Supplied as a 0.2 µm filtered PBS solution (pH 7.2) or lyophilized with stabilizers, it is easy to use and stable, with storage at 2-8°C for short-term or -20°C for long-term, avoiding freeze-thaw cycles. Its ethical production, free from mouse ascites, aligns with modern research standards, making it a compelling choice for labs prioritizing quality and sustainability.

To drive purchasing decisions, the Mouse IgG1 Isotype Control (Clone: 12B9) is rigorously tested for minimal background staining across human and mouse tissues, backed by a 100% satisfaction guarantee and detailed technical data sheets from suppliers like us. Competitively priced (starting at >$36 for 1 mg) and available in flexible sizes (50 µg, 100 µg, 1 mg), it fits various research budgets. Fast shipping and responsive customer support ensure seamless integration into experiments, while its proven performance in applications like flow cytometry and IHC makes it a go-to for researchers seeking reliable results.

Please contact us to ask for a quote for the Fc silenced mouse IgG1 isotype control mutants, mIgG1 isotype control and mutants with Avi-, His-, and Flag-tags, and biotinylated mIgG1 isotype control and mutants. A variety of conjugates (such as dyes and fluorophores) with the mouse IgG1 isotype control and Fc-slient mutants are available.

Citations of Syd Labs Mouse IgG1 Isotype Control Antibody:

1、In vivo targeting of open prothrombin with the monoclonal antibody POmAb results in anticoagulation without excessive bleeding
Brake MA, et al. J Thromb Haemost. 2026 Feb 13:S1538-7836(26)00111-X. doi: 10.1016/j.jtha.2026.01.027. Epub ahead of print. PMID: 41692091.
“ …… The in vivo grade recombinant mouse IgG1 antibody from Syd Labs (PA007126) was used as a control in all the in vitro, ex vivo, and in vivo studies …… ”

2、Intravenous anti-Aβ immunotherapy acutely increases cerebral amyloid angiopathy and vascular damages in APOE4 mice
Rebeck GW, et al. bioRxiv [Preprint]. 2026 Feb 11:2026.02.09.704876. doi: 10.64898/2026.02.09.704876. PMID: 41727014; PMCID: PMC12918975.
“ 3D6 Immunotherapy treatment paradigm：Mice have been divided into 8 groups (N=7-8 each): APOE3 mice treated with IgG1 isotype control, APOE3 mice treated with 5mg/kg IV 3D6 …… Four-month-old mice received a single 5mg/kg IV injection of either 3D6 or IgG1 isotype control antibody (Syd Labs cat #PA007126) into the lateral tail vein …… The other hemisphere was snap frozen on dry ice and stored at −80°C for biochemical analyses. The fixed hemisphere was sliced into 30μm sections on microtome for immunofluorescence (IF) experiments.”

3、POmAb, an antibody targeting open prothrombin, results in anticoagulation without excessive bleeding in mice
Pozzi N, et al. bioRxiv [Preprint]. 2025 Jul 4:2025.06.29.662207. doi: 10.1101/2025.06.29.662207. Update in: J Thromb Haemost. 2026 Feb 13:S1538-7836(26)00111-X. doi: 10.1016/j.jtha.2026.01.027. PMID: 40631305; PMCID: PMC12236754.
“ POmAb was expressed in CHO cells and purified as described previously¹⁰. Purity was >95% (4–12% SDS-PAGE under non-reducing conditions) and 99% (SEC-HPLC). Endotoxin level was < 0.1 EU/mg. POmAb concentration was calculated using the following molecular weight and extinction coefficient (E_280nm^0.1%): 150,000 Da, 1.58. In vivo grade recombinant mouse IgG₁ isotype control antibody was from Syd Labs (PA007126). Antibodies were aliquoted and stored at -80°C until used. Fluorescently labeled POmAb was prepared by conjugating 1.3 mg of antibody in 0.5 mL of 50 mM sodium borate buffer (pH 8.5) with DyLight 650-NHS ester. Protein/dye ratio was 3.27 moles of dye per mole of protein. Anti-fibrin antibody was purified from a growing hybridoma clone 59D8. Anti-CD42b beta antibody for staining platelets was from Emfret Analytics. All other reagents were purchased from Invitrogen (Carlsbad, CA) and Sigma (St. Louis, MO) unless otherwise stated.”

For more references about Mouse IgG1 Isotype Control Antibody please contact our scientific support team with message@sydlabs.com.

Fc ELISA Kits and Reagents:
Human Fc ELISA Kit
Mouse Fc ELISA Kit
Human Fc ELISA Reagent Kit
Mouse Fc ELISA Reagent Kit

Mouse IgG1 Isotype Control (12B9) from: In Vivo Grade Recombinant Mouse IgG1 Isotype Control Antibody PA007126: Syd Labs

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Mouse IgG1 Isotype Control Antibody | PA007126

Mouse IgG1 Isotype Control Antibody | PA007126