Rat IgG2b Fc Protein | BP4127

Name: Rat IgG2b Fc Protein | Ushelf
Brand: Syd Labs
SKU: BP4127
Price: 500 USD
Availability: InStock
Rating: 5 (1 reviews)

$500.00

Details & Specifications
References

Catalog No.	BP4127
Product Name	In Vivo Grade Recombinant Rat IgG2b Fc Protein
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Rat Fc protein (IgG2b), Rat IgG2b Fc region, Recombinant rtIgG2b Fc Protein
Summary	The In Vivo Grade Recombinant Rat IgG2b Fc Protein was produced in mammalian cells and purified by protein G. The rtIgG2b Fc protein includes the CH2 and CH3 domains of the heavy chain constant region as well as the hinge region before the CH2 domain.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

BP4127: Recombinant Rat IgG2b Fc Protein, In Vivo Grade

Background

In vivo grade recombinant natural and engineered mouse IgG2c Fc proteins (mIgG2c Fc), and recombinant mouse IgG2c isotype control antibody and mutants are available. Biotinylated mIgG2c Fc, and mIgG2c Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered mouse IgG2c Fc proteins, biotinylated mIgG2c Fc, and mIgG1 Fc with Avi-, His-, and Flag-tags. The recombinant mouse IgG2c Fc protein and mutants can be used as the isotype control of the mIgG2c antibodies and the mIgG2c Fc fusion proteins in various in vitro and in vivo experiments.

The in vivo grade recombinant mouse IgG2c Fc protein (mIgG2c Fc, Glu 98 – Lys 335 of the constant region of the IgG2c heavy chain, Accession # F6TQW2) was produced in mammalian cells and purified by protein G. The mIgG2c Fc protein includes the CH2 (126-225) and CH3 (234-330) domains of the heavy chain constant region as well as the hinge region (98-125) before the CH2 domain.

References of Rat IgG2b Fc Protein

1. Specificity of mouse and human Fcgamma receptors and their polymorphic variants for IgG subclasses of different species.
Wang, Y., et al.Eur J Immunol. 2022 May;52(5):779-792. PMID: 35188671
“Mice produce four IgG subclasses (IgG1, IgG2a/c, IgG2b, IgG3) and express four classical IgG receptors. Rat IgG2b complexes bind to all mouse FcγRs, rat IgG1 complexes bind exclusively to mFcγRIIB and mFcγRIII, and rat IgG2a complexes show no binding to mFcγRs except for a weak interaction with mFcγRIII. The study provides a comprehensive analysis of IgG subclass interactions across 12 species with human and mouse FcγRs, building on prior studies that documented intra-species binding.”

2. Rat IgG mediated circulatory cell depletion in mice requires mononuclear phagocyte system and is facilitated by complement.
Sun, Y., et al.J Leukoc Biol. 2020 Sep;108(3):997-1008. PMID: 31975410
“Rat monoclonal antibodies, including anti-C5aR (20/70, IgG2b), anti-VLA4 (PS/2, IgG2b), anti-CD11b (M1/70, IgG2b), and anti-CD11a (M17/4, IgG2a), were tested for cell depletion effects in mice. The study demonstrates that Ab-mediated cell depletion, reliant on the Fc portion, is an efficient tool for studying cell function under normal and disease conditions. The mononuclear phagocyte system and complement facilitate depletion, with Rat IgG2b antibodies showing significant activity.”

3. A general requirement for FcγRIIB co-engagement of agonistic anti-TNFR antibodies.
Zhang, L., et al.J Immunol. 2012 Sep 15;189(6):3004-3011. PMID: 22851718
“Mouse IgG1 and rat IgG2a Fcs preferentially bind to mouse FcγRIIB, while mouse IgG2a and rat IgG2b Fcs bind to activating FcγRs. A non-depleting rat IgG2a Fc anti-mouse CD27 antibody was more effective than a depleting rat IgG2b Fc clone in antitumor responses against CD27+ and CD27- EL4 syngeneic tumors. The study supports a general requirement for FcγRIIB co-engagement, rather than activating FcγRs, for agonistic anti-TNFR antibodies, highlighting the role of Fc interactions in therapeutic efficacy.”

4. Efficient Generation of Bispecific Murine Antibodies for Pre-Clinical Investigations in Syngeneic Rodent Models.
Haakonsen, B. S., et al.Sci Rep. 2017 Jun 2;7(1):2845. PMID: 28572631
“Bispecific antibodies (bsAbs) were generated by fusing mouse IgG2a and rat IgG2b hybridomas, exploiting species-restricted heavy-light chain pairing and differential Protein A elution to increase yield (mean recovery 75.2%). The study evaluated mutations in rat IgG1, IgG2a, IgG2b, and IgG2c backbones, confirming their applicability. This approach simplifies purification and enhances the production of bsAbs for pre-clinical studies in syngeneic rodent models.”

For more technical references or data sheets regarding the In Vivo Grade Recombinant Rat IgG2b Fc Protein formats, please contact our scientific support team at message@sydlabs.com.