Mouse IgG2b Fc Protein | BP4121

$500.00

Details & Specifications
References

Catalog No.	BP4121
Product Name	In Vivo Grade Recombinant Mouse IgG2b Fc Protein
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Mouse Fc protein (IgG2b), Mouse IgG2b Fc region, Recombinant mIgG2b Fc Protein
Summary	The In Vivo Grade Recombinant Mouse IgG2b Fc Protein was produced in mammalian cells and purified by protein A. The mIgG2b Fc protein includes the CH2 (126-225) and CH3 (234-330) domains of the heavy chain constant region as well as the hinge region (98-125) before the CH2 domain.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

BP4121: Recombinant Mouse IgG2b Fc Protein, In Vivo Grade

Background

In vivo grade recombinant natural and engineered mouse IgG2b Fc proteins (mIgG2b Fc), and recombinant mouse IgG2b isotype control antibody and mutants are available. Biotinylated mIgG2b Fc, and mIgG2b Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered mouse IgG2b Fc proteins, biotinylated mIgG2b Fc, and mIgG2b Fc with Avi-, His-, and Flag-tags. The recombinant mouse IgG2b Fc protein and mutants can be used as the isotype control of the mIgG2b antibodies and the mIgG2b Fc fusion proteins in various in vitro and in vivo experiments.

The in vivo grade recombinant mouse IgG2b Fc protein (mIgG2b Fc, Glu 97 – Lys 335 of the constant region of the IgG2b heavy chain, Accession # P01867-2) was produced in mammalian cells and purified by protein A. The mIgG2b Fc protein includes the CH2 (126-225) and CH3 (234-330) domains of the heavy chain constant region as well as the hinge region (98-125) before the CH2 domain.

References of Mouse IgG2b Fc Protein

1. Specificity of mouse and human Fcgamma receptors and their polymorphic variants for IgG subclasses of different species.
Wang, S., et al.Eur J Immunol. 2022 Jun;52(6):873-884. PMID: 35224703
“Mouse IgG subclasses interact with mouse Fcγ receptors (mFcγRs), with IgG1 binding to mFcγRIIB and mFcγRIII, IgG2a and IgG2c binding to all mFcγRs, IgG2b binding to mFcγRIIB, mFcγRIII, and mFcγRIV, and IgG3 binding to mFcγRI. Unexpectedly, mIgG3 complexes showed detectable binding to mFcγRIII in immune complex form and weak binding to mFcγRIV as Fab2C. Mouse IgG1 and IgG2b complexes bound predominantly to hFcγRIIAR131 and hFcγRIIAH131, weakly to hFcγRI and hFcγRIIB, while IgG2a and IgG2c bound to all hFcγRs except hFcγRIIIB variants, and IgG3 bound to hFcγRI and weakly to hFcγRIIIAV158.”

2. Cross-reactivity of mouse IgG subclasses to human Fc gamma receptors: Antibody deglycosylation only eliminates IgG2b binding.
Overdijk, M. B., et al.Mol Immunol. 2020 Nov;127:88-96. PMID: 32889346
“Mouse IgG subclasses cross-react with human Fc gamma receptors (hFcγRs), showing substantial functional conservation despite interspecies differences. Binding of human IgG to mouse FcγRs has been used to study effector functions in mice, but mouse IgG binding to human FcγRs can cause misinterpretation in applications like immunostaining. This study documents that deglycosylation eliminates binding only for mouse IgG2b, highlighting its unique interaction profile with hFcγRs.”

3. Relative Quantitation of Subclass-Specific Murine IgG Fc N-Glycoforms by Multiple Reaction Monitoring.
Li, Y., et al.ACS Omega. 2020 Jan 28;5(3):1415-1425. PMID: 32001040
“N-Linked glycosylation of the Fc domain of mouse IgG is a significant modulator of antibody functions and is subclass-specific. This study used multiple reaction monitoring to quantify subclass-specific peptides and Fc N-glycopeptides for IgG1*, IgG2b/2b*, IgG2a/2c, and IgG3 in mixed mouse serum, prepared by adding mouse IgG standard to C57BL/6 mouse serum. All peptides except IgG2aA were detected, aiding the study of how Fc glycosylation affects IgG subclass effector functions in various physiological or pathological states.”

4. FcγRIV: A Novel FcR with Distinct IgG Subclass Specificity.
Tao, M. H., et al.Immunity. 2005 Aug;23(2):139-48. PMID: 16107918
“Mouse IgG2a and IgG2b exhibit the greatest protective and pathogenic properties among mouse IgG subclasses, partly due to their binding to FcγRIV, a novel γ chain-dependent activating Fc receptor. FcγRIV, expressed on myeloid lineage cells, binds IgG2a and IgG2b with intermediate affinity but shows no binding to IgG1 or IgG3. Blocking FcγRIV binding to pathogenic anti-platelet antibodies protected mice from antibody-induced thrombocytopenia.”

5. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose.
Ey, P. L., et al.Mol Immunol. 1978 Jun;15(6):563-7. PMID: 98765
“A simple and rapid method for isolating pure mouse IgG1, IgG2a, and IgG2b immunoglobulins in nearly 100% yield is described. Mouse serum was fractionated on protein A-Sepharose 4B, with IgM, IgA, and IgE recovered in the effluent at pH 8.0. IgG subclasses were retained at pH 8.0 and sequentially eluted at pH 6.0-7.0 (IgG1), pH 4.5-5.0 (IgG2a), and pH 3.5-4.0 (IgG2b) using buffers of decreasing pH, provided the column’s binding capacity was not exceeded.”

For more technical references or data sheets regarding the Mouse IgG2b Fc Protein formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.