Human IgG1 Fc Protein | BP4091

$120.00 – $900.00

In vivo grade recombinant natural and engineered human IgG1 Fc proteins (hIgG1 Fc), and recombinant human IgG1 isotype control antibody and mutants are available. Biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags are also available.

Details & Specifications
References

Catalog No.	BP4091
Product Name	In Vivo Grade Recombinant Human IgG1 Fc Protein
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	Human Fc protein (IgG1), Human IgG1 Fc region, Recombinant hIgG1 Fc Protein
Summary	The In Vivo Grade Recombinant Human IgG1 Fc Protein was produced in mammalian cells and purified by protein A. The hIgG1 Fc protein includes the CH2 (111-223) and CH3 (224-330) domains of the heavy chain constant region as well as the hinge region (99-110) before the CH2 domain. The C103S version of the hIgG1 Fc protein is also available. The C103S substitution prevents improper cysteine bond formation in the absence of a light chain.
Form Of Antibody	0.2 μM filtered solution of 1x PBS.
Endotoxin	Less than 1 EU/mg of protein as determined by LAL method.
Purity	>95% by SDS-PAGE under reducing conditions
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

BP4091: Recombinant Human IgG1 Fc Protein, In Vivo Grade

Background

The recombinant human IgG1 Fc protein (hIgG1 Fc) includes the CH2 and CH3 domains of the heavy chain constant region. Most of the therapeutic antibody drugs and Fc fusion proteins carry the natural human IgG1 Fc region or engineered mutants. The recombinant human IgG1 Fc protein can be used as the isotype control of the hIgG1 antibodies and the hIgG1 Fc fusion proteins in various in vitro and in vivo experiments.

The in vivo grade recombinant human IgG1 Fc protein (hIgG1 Fc, Glu 99 – Lys 330 of the constant region of the IgG1 heavy chain, Accession # P01857-1) was produced in mammalian cells and purified by protein A. The hIgG1 Fc protein includes the CH2 (111-223) and CH3 (224-330) domains of the heavy chain constant region as well as the hinge region (99-110) before the CH2 domain. The C103S version of the hIgG1 Fc protein is also available. The C103S substitution prevents improper cysteine bond formation in the absence of a light chain.
Molecular weight: Predicted 26.1 kDa. Due to glycosylation, the recombinant human IgG1 Fc protein migrates to 32-34 kDa based on the Bis-Tris PAGE result.

Recombinant natural and engineered human IgG1 Fc proteins, and recombinant human IgG1 isotype control antibody and mutants are available. Biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags are also available. Please contact us to ask for a quote for the engineered human IgG1 Fc proteins, biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags.

References of Human IgG1 Fc Protein

1. A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice.
Powell, A. E., et al.ACS Cent Sci. 2021 Jan;7(1):183-199. PMID: 33527087
“For purified mAbs and ACE2-Fc, Direct-Blot HRP antihuman IgG1 Fc antibody was added at a 1:10,000 dilution in diluent buffer for 1 h at room temperature. …ACE2 binding was measured with Direct-Blot HRP antihuman IgG1 Fc at a 1:10,000 dilution, and blocking was quantified as the decrease in observed ACE2 binding as a function of serum dilution. …The development of a safe and effective SARS-CoV-2 vaccine is a public health priority. …Ferritin is an attractive nanoparticle platform for production of vaccines, and ferritin-based vaccines have been investigated in humans in two separate clinical trials. …Additionally, a single dose of SΔC-Fer elicited significantly higher neutralizing responses as compared to immunization with the spike receptor binding domain (RBD) monomer or spike ectodomain trimer alone.”

2. Development of a novel, fully human, anti-PCSK9 antibody with potent hypolipidemic activity by utilizing phage display-based strategy.
Xu, M., et al.EBioMedicine. 2021 Mar;65:103250. PMID: 33647772
“Thereafter, it was transformed to a full-length Fc-silenced anti-PCSK9 antibody FAP2M21 by fusing to a modified human IgG1 Fc fragment with L234A/L235A/N297G mutations and C-terminal lysine deletion, thus eliminating its immune effector functions and mitigating mAb heterogeneity. …Thereafter, considering the full-length IgG mAb being the most appropriate format for clinical applications because of its long half-life, high stability and bivalent binding property [64,65], we further constructed the full-length Fc-silenced anti-PCSK9 antibodies by fusing two anti-PCSK9 scFv variants (AP2M18 and AP2M21) to a modified human IgG1 Fc fragment with L234A/L235A/N297G mutations and C-terminal lysine deletion, thereby eliminating the immune effector functions and mitigating the potential mAb heterogeneity. …In summary, we generate a novel, fully human, anti-PCSK9 IgG1 mAb FAP2M21 with a high affinity to PCSK9 and an extremely slow dissociation rate by screening a fully human scFv phage display library, in vitro affinity maturation as well as fusing with a modified human IgG1 Fc fragment. …Proprotein convertase subtilase/kexin type 9 (PCSK9) regulates serum LDL cholesterol (LDL-C) levels by facilitating the degradation of the LDL receptor (LDLR) and is an attractive therapeutic target for hypercholesterolemia intervention. …A potent single-chain variable fragment (scFv) named AP2M21 was obtained by screening a fully human scFv phage display library with hPCSK9, and performing two in vitro affinity maturation processes including CDR-targeted tailored mutagenesis and cross-cloning.”

3. A Novel Splice Variant of Human TGF-β Type II Receptor Encodes a Soluble Protein and Its Fc-Tagged Version Prevents Liver Fibrosis in vivo.
Bertolio, M. S., et al.Front Cell Dev Biol. 2021 Sep;9:690397. PMID: 34568316
“TβRII-SE and human IgG1 Fc domain were fused in frame in a lentiviral vector (Lv) for further characterization. …Either coTβRII-SE/Fc or TβRII-EC was ligated to the human IgG1 Fc coding sequence and cloned into a self-inactivating (SIN) bicistronic lentiviral vectors making Lv.TβRII-SE/Fc and Lv.TβRII-Fc, respectively. …The human IgG1 Fc coding sequence was obtained by RT-PCR from peripheral blood leucocyte mRNA using specific oligonucleotides as primers (forward: 5′-AGATCTGACAAAACTCACACATGC-3′ and reverse: 5′-GATATCTTTACCCGGAGACAGG-3′), containing a BglII recognition site (forward primer) and an EcoRV site (reverse primer). …Transforming growth factor-β (TGF-β) is a multifunctional cytokine involved in critical processes, including immune regulation and wound healing together with cell proliferation, maturation, and differentiation. …Ligand binding to the receptor ectodomains induces conformational changes at the ligand–receptor interface, bringing their cytoplasmic domains closer.”

For more technical references or data sheets regarding the Human IgG1 Fc Protein formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.