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Biotinylated Anti-human HLA-ABC Antibody (W632, Human IgG1 Fc Silent Kappa) PA007766.h1Fs-b Syd Labs | Ushelf
Biotinylated Anti-human HLA-ABC Antibody (W632, Human IgG1 Fc Silent Kappa)

Biotinylated Anti-human HLA-ABC Antibody (W6/32, Human IgG1 Fc Silent Kappa)

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Biotinylated Anti-human HLA-ABC Antibody (W632, Human IgG1 Fc Silent Kappa) PA007766.h1Fs-b Syd Labs | Ushelf

Biotinylated Anti-human HLA-ABC Antibody (W6/32, Human IgG1 Fc Silent Kappa)

$70.00 – $140.00

In stock
Catalog No.: PA007766.h1Fs-b | Isotype: Human IgG1 Fc Silent Kappa | Flow Cytometry

$70.00 – $140.00

Recombinant anti-human HLA-ABC monoclonal antibody (Clone W6/32), engineered with a Human IgG1 (Fc-Silent) domain and conjugated to Biotin. Variable regions are derived from the classic mouse anti-human HLA-ABC clone (W6/32) and recombinantly expressed in mammalian cells to minimize non-specific Fc-receptor binding during flow cytometry analysis.

  • Endotoxin Level: < 1.0 EU/mg
  • Applications: Flow Cytometry (FC), Cell Surface Staining, Functional Assays
  • Alternative Formats: Unconjugated, alternative Fc isotypic engineered variants, and fluorophore-conjugated versions of clone W6/32 are also available.
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Order Offline:
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Catalog No. PA007766.h1Fs-b
Product Name Biotinylated Recombinant Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32), Human IgG1 Fc Silent Kappa
Supplier Name Syd Labs, Inc.
Brand Name Syd Labs
Clone W6/32.
Isotype Human IgG1 Fc Silent Kappa.
Source/Host The site-directed biotinylated anti-human HLA-ABC monoclonal antibody (clone: W6/32) was produced in mammalian cells.
Specificity/Sensitivity The site-directed biotinylated recombinant W6/32 antibody specifically binds to human HLA-ABC.
Applications Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate Biotin-conjugated.
Purity >95% by SDS-PAGE under reducing conditions.
Shipping The W6/32 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note

Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.

Order Offline Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007766.h1Fs-b: Biotinylated Recombinant Anti-human HLA-ABC Monoclonal Antibody (Clone: W6/32), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32)

1. Mass spectrometric profiling of HLA-B44 peptidomes provides evidence for tapasin-mediated tryptophan editing

Kaur, A., et al. J Immunol. 2023. PMID: 37737643

“This study uses mass spectrometry to explore tapasin-mediated tryptophan editing in HLA-B44 peptidomes. The W6/32 antibody (anti-human HLA-ABC) was purified from mouse ascites and crosslinked to PureProteome Protein A magnetic beads for immunoaffinity purification. Cells were stained with W6/32-FITC antibody for surface HLA class I analysis via quantitative flow cytometry, revealing a significant reduction in HLA-B44:05 molecules on tapasin knockout cells compared to wild-type cells. A slight, non-significant decrease in HLA-B44:05 surface stability was observed in tapasin knockout cells in the presence of brefeldin A.”

2. Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteins

Zhang, F., et al. Proc Natl Acad Sci U S A. 2022. PMID: 36136978

“This study investigates how sarbecovirus ORF7a proteins inhibit MHC-I antigen presentation. Human 293T, HuH7.5, U2OS, and A549/ACE2 cells were transduced with lentiviral vectors expressing SARS-CoV-2 ORF7a or Nef and stained with the pan-HLA-ABC antibody W6/32 for flow cytometry. MHC-I surface levels, measured by median fluorescence intensity, were reduced in ORF7a-expressing cells. The study compared wild-type and ORF7-deleted SARS-CoV-2 strains, confirming ORF7a’s role in suppressing MHC-I expression.”

3. Assessments of HLA-I Specificities of Anti-HLA-I Monoclonal Antibodies Using Solid Phase Bead Arrays

Zaitouna, A. J., et al. Bio Protoc. 2020. PMID: 33659325

“This study evaluates anti-HLA-I monoclonal antibody specificities using solid phase bead arrays. The W6/32 antibody, a pan anti-HLA class I antibody, was used to normalize HLA-I protein levels on Labscreen and FlowPRA Single Antigen Beads. Biotinylated W6/32 was tested at various dilutions (undiluted to 1:100) alongside anti-Bw4 and anti-Bw6 antibodies. Representative data showed W6/32 binding to HLA-I beads, with isotype control IgG2a used for comparison.”

4. Strategies for the measurements of expression levels and half-lives of HLA class I allotypes

Raghavan, M., et al. Hum Immunol. 2019. PMID: 30735755

“This study describes methods for measuring HLA class I expression and half-lives using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry histograms showed W6/32 signals for monocytes, NK cells, B cells, CD8+ T cells, and CD4+ T cells, with geometric mean values quantifying HLA class I expression. The W6/32 antibody was chosen for its broad recognition of most HLA class I allotypes, facilitating consistent assessment across healthy donors.”

5. Higher human lymphocyte antigen class I expression in early-stage cancer cells leads to high sensitivity for cytotoxic T lymphocytes

Akazawa, Y., et al. Cancer Sci. 2019. PMID: 30973665

“This study examines HLA class I expression in early-stage hepatocellular carcinoma (HCC) using the W6/32 antibody (anti-human HLA-ABC). Flow cytometry analysis of tissue from five HCC patients compared HLA class I expression between cancer cells and non-tumorous hepatocytes. The mean fluorescence intensity ratio of W6/32 staining indicated higher HLA class I expression in cancer cells, enhancing sensitivity to cytotoxic T lymphocytes. Heterogeneous expression was observed in non-tumorous hepatocytes in one case.”

For more technical references or data sheets regarding the Recombinant Anti-Human HLA-ABC Monoclonal Antibody (Clone: W6/32) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following recombinant MHC/HLA monoclonal antibodies:
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2) Monoclonal Antibodies (Clone M1/42.3.9.8.HLK)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Db) Monoclonal Antibodies (Clone 28-14-8S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone Y-3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kb) Monoclonal Antibodies (Clone AF6-88.5.3)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd, H-2Dd) Monoclonal Antibodies (Clone 34-1-2S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kd) Monoclonal Antibodies (Clone SF1-1.1.10)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 15-3-1S)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk, H-2Dk) Monoclonal Antibodies (Clone 16-1-2N)
In Vivo Grade Recombinant Anti-mouse MHC Class I (H-2Kk) Monoclonal Antibodies (Clone AF3-12.1.3)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A) Monoclonal Antibodies (Clone Y-3P)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-A/I-E) Monoclonal Antibodies (Clone M5/114.15.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ak, I-Ar, I-Af, I-As, I-Ag7) Monoclonal Antibodies (Clone 10-3.6.2)
In Vivo Grade Recombinant Anti-mouse MHC Class II (I-Ek/RT1-D) Monoclonal Antibodies (Clone 14-4-4S)
In Vivo Grade Recombinant Anti-mouse MHC Class II (Beta chain) Monoclonal Antibodies (Clone KL277)
In Vivo Grade Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32)
In Vivo Grade Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243)
In Vivo Grade Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3)
In Vivo Grade Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10)
In Vivo Grade Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10)
In Vivo Grade Recombinant Anti-human MHC Class II HLA-DP/DQ/DR Monoclonal Antibodies (Clone IVA12)
Recombinant Anti-human HLA-ABC Monoclonal Antibodies (Clone W6/32) for flow cytometry
Recombinant Anti-human HLA-DR Monoclonal Antibodies (Clone L243) for flow cytometry
Recombinant Anti-human HLA-DR/DP/DQ Monoclonal Antibodies (Clone F3.3) for flow cytometry
Recombinant Anti-human HLA Class I Heavy Chain Monoclonal Antibodies (Clone HC10) for flow cytometry
Recombinant Anti-human HLA class II DR/DQ Monoclonal Antibodies (Clone 9.3F10) for flow cytometry

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