Biotinylated Anti-mouse CD4 Antibody (GK1.5, Human IgG1 Fc Silent Kappa)

Catalog No.	PA007723.h1Fs-b
Product Name	Biotinylated Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: GK1.5), Human IgG1 Fc Silent Kappa
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Clone	GK1.5.
Isotype	Human IgG1 Fc Silent Kappa.
Source/Host	The site-directed biotinylated anti-mouse CD4 monoclonal antibody (clone: GK1.5) was produced in mammalian cells.
Specificity/Sensitivity	The site-directed biotinylated recombinant GK1.5 antibody specifically binds to mouse T cell receptor CD4.
Applications	Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Conjugate	Biotin-conjugated.
Purity	>95% by SDS-PAGE under reducing conditions and HPLC
Shipping	The GK1.5 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant rat IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007723.h1Fs-b: Biotinylated Recombinant Anti-mouse CD4 Monoclonal Antibody (Clone: GK1.5), Human IgG1 Fc Silent Kappa

Recombinant human IgG1 Monoclonal Antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.

References of Anti-Mouse CD4 Monoclonal Antibody (Clone: GK1.5)：

1. ImmunoPET Imaging of Murine CD4+ T Cells Using Anti-CD4 Cys-Diabody: Effects of Protein Dose on T Cell Function and Imaging

Freise, A. C., et al. Mol Imaging Biol. 2017 Aug;19(4):599-609. PMID: 27966069

“In vivo administration of GK1.5 cDb caused a transient decrease in CD4 expression in spleen, blood, lymph nodes, and thymus, while concentrations in excess of 25 nM significantly inhibited CD4+ T cell proliferation and interferon-γ production in vitro.”

2. Migrant memory B cells secrete luminal antibody in the vagina

Oh, J. E., et al. Nature. 2019 Jul;571(7763):122-126. PMID: 31189952

“To deplete CD4 T cells, mice were treated with 300 μg intravenously on day -4, -1 and 10 μg anti-mouse CD4 Ab (GK1.5) intravaginally for four consecutive days before virus challenge.”

3. Effect of a CD4-depleting antibody on the development of Cryptococcus neoformans-induced allergic bronchopulmonary mycosis in mice

Arora, S., et al. Infect Immun. 2006 Jul;74(7):4339-48. PMID: 16790808

“Mice were treated with 300 μg of anti-CD4 monoclonal antibody GK1.5 intraperitoneally on the day prior to C. neoformans inoculation and boosted with 100 μg of MAb at days 7 and 14.”

4. Protocol for the isolation of mouse senescence-associated CD4+ T cells using flow cytometry and functional assays

Fukushima, Y., et al. STAR Protoc. 2023 Sep 15;4(3):102472. PMID: 37515759

“Anti-CD4 (GK1.5), FITC (1:200) and Anti-CD4 (GK1.5), APC-Cy7 (1:200) are listed in the Key resources table under Antibodies for antibody staining panels to isolate CD153+ SA-T cells.”

5. T4+ T helper cell function in vivo: differential requirement for induction of antiviral cytotoxic T-cell and antibody responses

Ahmed, R., et al. J Virol. 1988 Jun;62(6):2102-2106. PMID: 2966865

“Two injections of GK1.5 (300 μg each on days -3 and -1) caused >90% depletion of L3T4+ cells in vivo.”

For more technical references or data sheets regarding the Recombinant Anti-Mouse CD4 Monoclonal Antibody (Clone: GK1.5) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies::
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS191)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS 177.9.6.1)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTA 3.1.2)

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies::
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)

Syd Labs provides the following recombinant anti-mouse CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5) for flow cytometry

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry