Biotinylated Anti-human CD4 Antibody (13B8.2, Human IgG1 Fc Silent Kappa)
$150.00 – $300.00
Biotinylated recombinant anti-human CD4 monoclonal antibody (Clone 13B8.2), engineered with a Human IgG1 Fc-silent domain to minimize non-specific background binding to Fc receptors during cell analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, with variable regions derived from anti-human CD4 clone (13B8.2) and expressed in mammalian cells.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Cell Isolation, Functional Assays
- Alternative Formats: Unconjugated mouse IgG2a and human IgG1 Fc-silent versions of clone 13B8.2 are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007758.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | 13B8.2 |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD4 monoclonal antibody (clone: 13B8.2) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant 13B8.2 antibody specifically binds to human CD4. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The 13B8.2 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG1 isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007758.h1Fs-b: Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: 13B8.2), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
The recombinant 13B8.2 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.
References of Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2):
Troadec, S., et al. J Immunother. 2007 Feb-Mar;30(2):190-202. PMID: 17273799
“The chimeric recombinant anti-CD4 antibody 13B8.2, expressed in baculovirus, was tested for antitumoral activity against T-cell lymphomas using flow cytometry. The 13B8.2 antibody induced apoptosis in CD4+ Jurkat cells, as confirmed by Annexin V staining, and significantly reduced CD4+ T cells in treated lymphoma cell lines. Cell viability was assessed after 48 hours using MTT assays, and the efficacy of 13B8.2 was compared with other anti-CD4 antibodies in vitro.”
Guillerm, C., et al. Virology. 1998 Sep 15;248(2):254-63. PMID: 9743188
“The anti-CD4 monoclonal antibody 13B8.2, targeting the CDR3-like region of CD4, was used to assess HIV-1 envelope glycoprotein-induced apoptosis via flow cytometry. Preincubation with 13B8.2 significantly reduced apoptosis in HIV-1-infected T cells and blocked CD4 crosslinking by gp120. The study also demonstrated that 13B8.2 inhibits syncytium formation in vitro, with cell surface CD4 expression quantified using fluorescently labeled antibodies.”
3. The Cytoplasmic Tail of CD4 Is Required for Inhibition of HIV-1 Env-Mediated Cell Fusion
Albright, C. A., et al. J Virol. 1997 Jun;71(6):4686-91. PMID: 9171443
“The 13B8.2 antibody (anti-human CD4 antibody) was used to detect CD4 expression on transfected HeLa cells by flow cytometry to study HIV-1 envelope-mediated cell fusion. Mutations in the CD4 cytoplasmic tail abolished the inhibitory effect on cell fusion, while flow cytometry confirmed that CD4 surface expression remained unchanged. HIV-1 envelope glycoprotein binding was assessed through co-culture experiments.”
Benkirane, M., et al. J Virol. 1995 Nov;69(11):6904-10. PMID: 7568058
“The anti-CD4 antibody 13B8.2, targeting the immunoglobulin CDR3-like region, was used to inhibit HIV-1 replication in Jurkat cells, with CD4 expression monitored by flow cytometry. The 13B8.2 antibody effectively blocked HIV-1 provirus transcription without significantly altering CD4 surface levels. Inhibition of HIV-1 entry was confirmed using syncytium formation assays.”
Benkirane, M., et al. EMBO J. 1993 Dec;12(12):4909-21. PMID: 8102412
“The 13B8.2 antibody (anti-human CD4 mAb), targeting the immunoglobulin CDR3-like region of CD4, was used to inhibit provirus transcription in HIV-1-infected Jurkat cells, with CD4 expression quantified by flow cytometry. The study showed that 13B8.2 does not affect HIV-1 entry but targets post-entry events, with stable CD4 expression confirmed during treatment.”
For more technical references or data sheets regarding the Recombinant Anti-Human CD4 Monoclonal Antibody (Clone: 13B8.2) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS191)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS 177.9.6.1)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTA 3.1.2)
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Syd Labs provides the following recombinant anti-mouse CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5) for flow cytometry
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
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