Biotinylated Anti-human CD4 Antibody (OKT4, Human IgG1 Fc Silent Kappa)
$40.00 – $80.00
Biotinylated recombinant anti-human CD4 monoclonal antibody (Clone OKT4), engineered with a Human IgG1 Fc-silent domain to minimize non-specific background binding to Fc receptors during cell analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, with variable regions derived from anti-human CD4 clone (OKT4) and expressed in mammalian cells.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Cell Isolation, Functional Assays
- Alternative Formats: Unconjugated mouse IgG2a and human IgG1 Fc-silent versions of clone OKT4 are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007754.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | OKT4 |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human CD4 monoclonal antibody (clone: OKT4) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant OKT4 antibody specifically binds to human CD4. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions. |
| Shipping | The OKT4 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007754.h1Fs-b: Biotinylated Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
The recombinant OKT4 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.
References of Anti-Human CD4 Monoclonal Antibody (Clone: OKT4):
1. Single amino acid substitution in the V3 domain of CD4 is responsible for OKT4 epitope deficiency
Kawamura, N., et al. Immunogenetics. 1991;34(3):208-10. PMID: 1716607
“The OKT4 antibody (anti-human CD4) was used to identify a deficiency in the CD4 epitope in patients with normal CD4+ T cell counts, as detected by other anti-CD4 monoclonal antibodies. This study linked a single amino acid substitution in the V3 domain of CD4 to the OKT4 epitope deficiency, relevant to understanding HIV pathogenesis as CD4 is the HIV receptor.”
Boylston, A. W., et al. Cell Immunol. 1982 Jul 15;71(1):12-26. PMID: 6183008
“The OKT4 antibody (anti-human CD4) was used in flow cytometry to analyze surface antigen changes in activated T lymphocytes. The study observed a shift in the OKT4:OKT8 ratio from 60:40 to 40:60 in cultured T lymphoblasts, suggesting a possible phenotype change from OKT4+ to OKT8+ cells, while OKT4 expression remained stable.”
Ferson, M., et al. Chest. 1982 Nov;82(5):526-9. PMID: 6982152
“The OKT4 antibody (anti-human CD4) was used in flow cytometry to assess T-cell subsets in heavy smokers, revealing a decreased percentage of OKT4+ cells and a reduced OKT4+/OKT8+ ratio. These changes normalized six weeks after smoking cessation, indicating reversible alterations in immunoregulatory T cells linked to smoking.”
Lyding, J., et al. J Fluoresc. 1991 Jun;1(2):141-6. PMID: 24242964
“The OKT4 antibody (anti-human CD4) was used in a quantitative flow cytometry assay with Simply Cellular microbeads to measure recombinant CD4 antigen levels. The assay, using standard conditions for OKT4 binding and detection, enabled epitope mapping and determination of CD4 oligomerization levels in solution.”
5. Asbestos exposure correlates with alterations in circulating T cell subsets
de Shazo, R. D., et al. Am J Med Sci. 1985 Jan;289(1):1-5. PMID: 3929128
“The OKT4 antibody (anti-human CD4) was used in flow cytometry to assess alterations in circulating T-cell subsets in asbestos-exposed individuals. The study identified changes in CD4+ T-cell populations, suggesting microbial metabolites like ascorbate influence CD4+ T-cell function, with implications for Th17-mediated conditions like Crohn’s disease.”
For more technical references or data sheets regarding the Recombinant Anti-Human CD4 Monoclonal Antibody (Clone: OKT4) formats, please contact our scientific support team at message@sydlabs.com or explore options via Syd Labs.
Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS191)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTS 177.9.6.1)
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: YTA 3.1.2)
Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Syd Labs provides the following recombinant anti-mouse CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5) for flow cytometry
Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-Leu 3a) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
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