Biotinylated Anti-human Siglec-2/CD22 Antibody (NCI m971, Human IgG1 Fc Silent Kappa)
$110.00 – $220.00
Biotinylated recombinant anti-human Siglec-2 (CD22) monoclonal antibody (Clone NCI m971), engineered with a Human IgG1 Fc-silent domain to minimize non-specific background binding to Fc receptors during cell analysis. Features site-directed biotinylation via a C-terminal Avi tag on the heavy chain, specifically targeting the membrane-proximal d7 domain of human CD22 with high binding specificity.
- Conjugation & Purity: Biotinylated (Site-directed via C-terminal Avi-tag), > 95% by SDS-PAGE
- Applications: Flow Cytometry (FC) via Streptavidin Conjugates, Cell Isolation, Functional Assays
- Alternative Formats: Unconjugated human IgG1 Fc-silent and other recombinant versions of clone NCI m971 are also available at Syd Labs and Ushelf.
- Details & Specifications
- References
| Catalog No. | PA007300.h1Fs-b |
|---|---|
| Product Name | Biotinylated Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971), Human IgG1 Fc Silent Kappa |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Clone | NCI m971. |
| Isotype | Human IgG1 Fc Silent Kappa. |
| Source/Host | The site-directed biotinylated anti-human Siglec-2/CD22 monoclonal antibody (clone: NCI m971) was produced in mammalian cells. |
| Specificity/Sensitivity | The site-directed biotinylated recombinant NCI m971 antibody specifically binds to the human Siglec-2/CD22 protein. |
| Applications | Flow cytometry (FC) with various dye conjugation through streptavidin; conjugation to streptavidin agarose beads or streptavidin magnetic beads. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Conjugate | Biotin-conjugated. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The NCI m971 antibody is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant mouse IgG2a isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5019 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007300.h1Fs-b: Biotinylated Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971), Human IgG1 Fc Silent Kappa
Recombinant human IgG1 monoclonal antibody with site-directed biotinylation through the Avi tag at the C-terminus of the antibody heavy chain.
The recombinant NCI m971 antibody binds to the human Siglec-2/CD22 protein, a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer’s patches in mice. In mice, it has been shown that CD22 blockade restores homeostatic microglial phagocytosis in aging brains.
References of Anti-CD22 Monoclonal Antibody (Clone: NCI m971):
1. Structural details of monoclonal antibody m971 recognition of the membrane-proximal domain of CD22
Ereño-Orbea, J., et al. J Biol Chem. 2021 Oct;296:100966. PMID: 34273351
“The NCI m971 antibody’s Fab fragment bound the CD22 d7 domain with low nanomolar affinity (25 nM by BLI, comparable to 75 nM by flow cytometry). Cocrystal structures showed a preconfigured paratope (RMSD 0.33 Å), with HCDRs 1–3 and LCDRs 1–2 interacting with CD22’s C β-strand and loops, unaffected by N-linked glycosylation.”
For more technical references or data sheets regarding the Anti-CD22 Monoclonal Antibody (Clone: NCI m971) formats, please contact our scientific support team at message@sydlabs.com.
Syd Labs provides the following recombinant anti-human Siglec-2/CD22 monoclonal antibodies for flow cytometry:
Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
Biotin Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
FITC Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
APC Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
Pacific Blue Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PerCP Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE/Cy5 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PE/Cy7 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 488 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 549 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 594 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 647 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
iFluor 700 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
APC/Cy7 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
PerCP/Cy5.5 Recombinant Anti-human Siglec-2/CD22 Monoclonal Antibody (Clone: NCI m971)
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