Catalog No.	PA007384.m2b
Product Name	Anti-human CD4 Antibody, Clone OKT4 | PA007384.m2b
Supplier Name	Syd Labs, Inc.
Brand Name	Syd Labs
Synonyms	cluster of differentiation 4, leu-3, T4
Clone	OKT4.
Isotype	Mouse IgG2b kappa.
Source/Host	The anti-human CD4 monoclonal antibody (clone: OKT4) was produced in mammalian cells.
Specificity/Sensitivity	The in vivo grade recombinant mouse monoclonal antibody (clone: OKT4) specifically binds to human CD4.
Applications	ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the human CD4 protein.
Form Of Antibody	0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.
Endotoxin	< 1 EU per 1 mg of the protein by the LAL method.
Purity	>95% by SDS-PAGE under reducing conditions.
Shipping	The In vivo Grade Recombinant Anti-human CD4 Monoclonal Antibody (Clone: OKT4), Mouse IgG2b Kappa is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied.
Note	Recombinant mouse IgG2b isotype controls and Recombinant human IgG1 isotype controls are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
Order Offline	Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card.

Description

PA007384.m2b: Recombinant Anti-human CD4 Monoclonal Antibody(Clone: OKT4), Mouse IgG2b Kappa, In vivo Grade

Background of Anti-human CD4 Monoclonal Antibody(Clone: OKT4)

The recombinant OKT4 antibody binds to the human CD4 protein (cluster of differentiation 4), a glycoprotein and the co-receptor for the T-cell receptor (TCR). The CD4 protein is expressed on the surface of immune cells such as helper T cells, monocytes, macrophages, and dendritic cells.

References of Anti-human CD4 Monoclonal Antibody:

1、Human Fcγ-receptor IIb modulates pathogen-specific versus self-reactive antibody responses in lyme arthritis.
Danzer, H., et al. eLife. 2020 Jul 2;9:e55319. doi: 10.7554/eLife.55319. PMID: 32613944
“To deplete T cells, 100 µg of the human CD4-specific antibody OKT4 (BioXcell) was injected intraperitoneally 3, 2 and 1 days before B. …Sera of humanized mice were obtained by collecting blood from the retro-orbital plexus. …Serum from control and B. burgdorferi infected humans was obtained by collecting venous blood in BD Vacutainer tubes at the Medical Department 3 of the University Hospital Erlangen. …For detection of B. burgdorferi-specific antibodies, ELISA plates were coated with B. burgdorferi ultrasonic lysate at 5 µg/ml (0.5 µg/well) over night at 4°C. …Antibodies against a predefined series of immunodominant proteins of B. burgdorferi sensu lato were measured using a commercial western blot approach in which purified recombinant B. burgdorferi antigens are coated on a membrane which is incubated with the sera of humanized mice (diluted 1:20), followed by detection of bound antibodies by human IgM-HRP coupled secondary antibodies.”

2、Response to Programmed Cell Death-1 Blockade in a Murine Melanoma Syngeneic Model Requires Costimulation, CD4, and CD8 T Cells.
“Homet Moreno, B., et al. Cancer Immunol Res. 2016 Oct;4(10):845-857. doi: 10.1158/2326-6066.CIR-16-0060. PMID: 27589875
To elucidate the role of CD8 and CD4 T cells in anti–PD-1 activity, both cell subtypes were depleted in C57BL/6 mice bearing MC38 or YUMM2.1 tumors. …Three and ten days after starting treatment with anti–PD-1 or isotype control, tumors and spleens were harvested and stained for CD3, CD4, and CD8 (Supplementary Fig. S2C and S2D). …The evidence that both CD4 and CD8 cells are required for response to PD-1 blockade in the MC38 and YUMM2. …We confirmed the essential roles of both the CD8 and CD4 T cells in anti–PD-1 effect in both MC38 and YUMM2.1 tumor models. …It is unknown if the location of PD-1/PD-L1 interaction and its consecutive blockade is tumor-dependent in a short-term implanted tumor model.”

3、T cell receptor dwell times control the kinase activity of Zap70.
Klammt, C., et al. Nat Immunol. 2015 Sep;16(9):961-9. doi: 10.1038/ni.3231. PMID: 26237552
“Kinase recruitment to membrane receptors is essential for signal transduction. …We investigated how conformational changes control T cell receptor (TCR) association and activity of the kinase Zap70. …Structural analysis showed that TCR binding or phosphorylation of Zap70 triggers a transition from a closed, autoinhibited conformation to an open conformation. …The closed conformation minimized TCR dwell times and thereby prevented activation by membrane-associated kinases. …Our study suggests that the dynamics of cytosolic enzyme recruitment to the plasma membrane regulate the activity and function of receptors lacking intrinsic catalytic activity.”

4、Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice.
Horwitz, J. A., et al. J Immunol. 2013 Aug 15;191(4):1753-64. doi: 10.4049/jimmunol.1201518. PMID: 23833235
“Humanized mice have emerged as a promising model to study human immunity in vivo. …It has recently been demonstrated that the expression of HLA molecules improves human immunity to lymphotropic virus infections in humanized mice. …However, little is known about the extent of functional human immune responses in nonlymphoid tissues, such as in the liver, and the role of HLA expression in this context. …Using a firefly luciferase–expressing adenovirus and in vivo bioluminescence imaging, we demonstrate a human T cell–dependent partial clearance of adenovirus-infected cells from the liver of HLA-transgenic humanized mice. …The adenovirus reporter system used in our study may serve as simple in vivo method to evaluate future strategies for improving human intrahepatic immune responses in humanized mice.”

5、Antibody-mediated stripping of CD4 from lymphocyte cell surface in patients with rheumatoid arthritis.
Hepburn, T. W., et al. Rheumatology (Oxford). 2003 Jan;42(1):54-61. doi: 10.1093/rheumatology/keg030. PMID: 12509613
“Clenoliximab, an immunoglobulin G4 derivative of keliximab, has substantially reduced potential to deplete CD4 cells. …In initial studies of clenoliximab, we investigated the hypothesis that the decrease in cell surface CD4 is the result of antibody‐mediated stripping from the cell surface. …Blood was collected for up to 16 weeks and pharmacokinetic and pharmacodynamic assessments were conducted using immunoassay and flow cytometry. …Maximal down‐modulation persisted for an increasing period as dose increased, while soluble CD4–clenoliximab complexes accumulated. …The amount of CD4 in soluble complex was as much as 20 times the amount of cell‐associated CD4.”

For more references about Anti-human CD4 Monoclonal Antibody please contact our scientific support team with message@sydlabs.com.

Syd Labs provides the following in vivo grade recombinant anti-human CD4 monoclonal antibodies:
Clenoliximab biosimilar, research grade, anti-human CD4 monoclonal antibody
Ibalizumab biosimilar, research grade, anti-human CD4 monoclonal antibody
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4)
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A)
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2)
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a)

Syd Labs provides the following in vivo grade recombinant anti-mouse CD4 monoclonal antibodies:
Recombinant Anti-mouse CD4 monoclonal antibody (Clone: GK1.5)

Syd Labs provides the following recombinant anti-human CD4 monoclonal antibodies for flow cytometry:
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: OKT4A) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: 13B8.2) for flow cytometry
Recombinant Anti-human CD4 monoclonal antibody (Clone: SK3 / Anti-LEU 3a) for flow cytometry

Anti-human CD4 (OKT4) from: Anti-human CD4 Monoclonal Antibody (Clone: OKT4): PA007384.m2b Syd Labs

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