Anti-mouse PLVAP/PV-1 Monoclonal Antibody (Clone: MECA-32) | PA007476.r2a
$150.00 – $900.00
Recombinant rat IgG2a isotype control antibody are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator.
- Details & Specifications
- References
| Catalog No. | PA007476.r2a |
|---|---|
| Product Name | In vivo Grade Recombinant Anti-mouse PLVAP/PV-1 Monoclonal Antibody, Rat IgG2a Kappa (Clone: MECA-32) |
| Data Sheet | 📄 Download Product Data Sheet (PDF) |
| Supplier Name | Syd Labs, Inc. |
| Brand Name | Syd Labs |
| Synonyms | PV1; Plasmalemma vesicle-associated protein; Plasmalemma vesicle protein 1 |
| Clone | MECA-32. |
| Isotype | Rat IgG2a Kappa. |
| Source/Host | The anti-mouse PLVAP/PV-1 monoclonal antibody (clone: MECA-32) was produced in mammalian cells. |
| Specificity/Sensitivity | The in vivo grade recombinant rat monoclonal antibody (clone: MECA-32) specifically binds to the mouse PLVAP/PV-1 protein. |
| Applications | ELISA, flow cytometry, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by the mouse PLVAP/PV-1 protein. |
| Form Of Antibody | 0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives. |
| Endotoxin | < 1 EU per 1 mg of the protein by the LAL method. |
| Purity | >95% by SDS-PAGE under reducing conditions and HPLC. |
| Shipping | The In vivo Grade Recombinant Anti-mouse PLVAP/PV-1 Monoclonal Antibody, Rat IgG2a Kappa (Clone: MECA-32) is shipped with ice pack. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1 month from date of receipt, 2 to 8°C as supplied. |
| Note | Recombinant rat IgG2a isotype control antibody are available. Condition of sample preparation and optimal sample dilution should be determined experimentally by the investigator. |
| Order Offline | Phone: 1-617-401-8149 Fax: 1-617-606-5022 Email: message@sydlabs.com Or leave a message with a formal purchase order (PO) Or credit card. |
Description
PA007476.r2a: Recombinant Anti-mouse PLVAP/PV-1 Monoclonal Antibody(Clone: MECA-32), Rat IgG2a Kappa, In vivo Grade
Recombinant Rat IgG2a Kappa Monoclonal Antibody.
References of Anti-mouse PLVAP/PV-1 Monoclonal Antibody (Clone: MECA-32)
1. Paclitaxel therapy promotes breast cancer metastasis in a TLR4-dependent manner.
Volk-Draper, L., et al.Cancer Res. 2014 Oct 1;74(19):5421-34. PMID: 25274031
“Primary antibodies were rat anti-mouse CD11b, Ly6C, Ly6G and MECA-32 (BioXCell, NH); rabbit anti-mouse Lyve-1 and Prox1 (Angiobio); mouse anti-human HLA (ATCC); and PE-rabbit anti-mouse Ly6C (BD Bioscience). …Blood vascular density (BVD) and lymphatic vessel density (LVD) were determined from four images per slide captured at ×200 magnification in tumor sections stained with either anti-MECA-32 or anti-Lyve-1 antibodies (3 mice/group). …Vascular structures were enumerated using ImageJ. BVD is presented as the mean number of vessels per field ± SEM, and LVD is the mean number of Lyve-1+ structures normalized per mm2 of the section area ± SEM. …Tumor sections were double-stained with anti-human HLA and anti-mouse MECA-32 or Lyve-1 antibodies as described above. Blood and lymphatic vessels with open lumens were identified and enumerated in each section. …BVD was determined in tumors collected on the 21st day posttreatment using antibody against MECA-32 (E).”
2. Caveolae, fenestrae and transendothelial channels retain PV1 on the surface of endothelial cells.
Tkachenko, E., et al.PLoS One. 2012;7(3):e32655. PMID: 22403691
“The hybridoma secreting the rat anti-mouse PV1 IgG2a mAb MECA-32 [6], [59] were from the Developmental Studies Hybridoma Bank, University of Iowa. …Rat anti-mouse PV1 IgG2a mAb, clone MECA-32 mAb was produced in serum free media by BioXCell, Lebanon, NH. …Antibodies used were either the rat anti-mouse PV1 MECA-32 mAb, the chicken anti-mouse PV1C pAb described here, rabbit anti caveolin 1 pAb, mouse anti-Cavin-1/PTRF, goat anti CD31 pAb, goat anti-VE Cadherin pAb and rabbit anti-Cavin 2/SDPR (Abcam). …RNA integrity and quality were determined using Bioanalyzer (Agilent) and NanoDrop (Thermo-Fisher). …Fluorescent rat IgG2a was used as isotype control for MECA-32 antibody.”
For more technical references or data sheets regarding the Anti-mouse PLVAP/PV-1 Monoclonal Antibody (Clone: MECA-32) formats, please contact our scientific support team at message@sydlabs.com.
Anti-mouse PLVAP/PV-1 Antibody (MECA-32) from: In vivo Grade Recombinant Anti-mouse PLVAP/PV-1 Monoclonal Antibody, Rat IgG2a Kappa (Clone: MECA-32): PA007476.r2a Syd Labs
